Reviewed, UniProtKB/Swiss-Prot Q4ULP1 (TRXB_RICFE)

Names and origin

Protein names

Recommended name:
    Thioredoxin reductase
      Short name=TRXR
    EC=1.8.1.9

Gene names

Name:	trxB
Ordered Locus Names:	RF_0681

Organism

Rickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

42862 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Thioredoxin + NADP+ = thioredoxin disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW removal of superoxide radicals Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro thioredoxin-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Thioredoxin reductase		PRO_0000274791
Regions
	Nucleotide binding	34 – 41	8	FAD By similarity
	Nucleotide binding	281 – 290	10	FAD By similarity
Amino acid modifications
	Disulfide bond	135 ↔ 138		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4ULP1-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: AAC4CEB2E55F3785	FASTA	310	33,382
	10 20 30 40 50 60 MKITTKVLII GSGPAGLSAA IYTARAALKP ILINGMQPGG QLTITTDVEN YPGFAETVQG 70 80 90 100 110 120 PWLMEQMSMQ AKNVGTEIIS DYVEKVDLSK RPFKVFTGAG NEYEAESIII CTGAEAKWLS 130 140 150 160 170 180 IASEQEFRGF GVSACATCDG FFFKNQEIVV VGGGNSAVEE ALYLTNHASK VTITHRRDSF 190 200 210 220 230 240 RAEKILQDRL FKNPKISVIW DHVVDEIVGS DKPKSVTGVK IQNVHTKEIS LVNCSGVFIA 250 260 270 280 290 300 IGHAPNTGLF TGQIAMDDDN YIITKSGTTK TSVEGVFAAG DVQDKIYRQA VTAAGSGCMA 310 ALEVEKFLNK

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000053 Genomic DNA. Translation: AAY61532.1.
RefSeq	YP_246697.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3401306.
GenomeReviews	Gene locus RF_0681 in contig CP000053_GR.
KEGG	rfe:RF_0681.
NMPDR	fig|315456.3.peg.592.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4ULP1.
OMA	Q4ULP1. HYIANSI.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_0681-MON.
BRENDA	1.8.1.9. 297363.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR008255. Pyr_nucl-diS_OxRdtase_2_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR000103. Pyridine_nuc-diS_OxRdtase_2. IPR005982. Thioredox_Rdtase. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00469. PNDRDTASEII.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01292. TRX_reduct. 1 hit.
PROSITE	PS00573. PYRIDINE_REDOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRXB_RICFE

Accession

Primary (citable) accession number: Q4ULP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	July 5, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)