ID   LON_RICFE               Reviewed;         778 AA.
AC   Q4ULN0;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   16-JUN-2009, entry version 33.
DE   RecName: Full=ATP-dependent protease La;
DE            EC=3.4.21.53;
GN   Name=lon; OrderedLocusNames=RF_0692;
OS   Rickettsia felis (Rickettsia azadi).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX   NCBI_TaxID=42862;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC VR-1525 / URRWXCal2;
RX   PubMed=15984913; DOI=10.1371/journal.pbio.0030248;
RA   Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E.,
RA   Parinello H., Claverie J.-M., Raoult D.;
RT   "The genome sequence of Rickettsia felis identifies the first putative
RT   conjugative plasmid in an obligate intracellular parasite.";
RL   PLoS Biol. 3:1-12(2005).
CC   -!- FUNCTION: Degrades short-lived regulatory and abnormal proteins in
CC       presence of ATP. Hydrolyzes two ATPs for each peptide bond cleaved
CC       in the protein substrate (By similarity).
CC   -!- CATALYTIC ACTIVITY: Hydrolysis of proteins in presence of ATP.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the peptidase S16 family.
CC   -!- SIMILARITY: Contains 1 Lon domain.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; CP000053; AAY61543.1; -; Genomic_DNA.
DR   RefSeq; YP_246708.1; -.
DR   MEROPS; S16.001; -.
DR   GeneID; 3400757; -.
DR   GenomeReviews; CP000053_GR; RF_0692.
DR   KEGG; rfe:RF_0692; -.
DR   NMPDR; fig|315456.3.peg.692; -.
DR   HOGENOM; Q4ULN0; -.
DR   OMA; Q4ULN0; VMKESIQ.
DR   BioCyc; RFEL315456:RF_0692-MON; -.
DR   BRENDA; 3.4.21.53; 297363.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004176; F:ATP-dependent peptidase activity; IEA:InterPro.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR   InterPro; IPR003593; ATPase_AAA+_core.
DR   InterPro; IPR003959; ATPase_AAA_core.
DR   InterPro; IPR008269; Pept_S16_C.
DR   InterPro; IPR004815; Pept_S16_lon.
DR   InterPro; IPR003111; Pept_S16_N.
DR   InterPro; IPR008268; Peptidase_S16_AS.
DR   InterPro; IPR001984; Peptidase_S16_C.
DR   Pfam; PF00004; AAA; 1.
DR   Pfam; PF02190; LON; 1.
DR   Pfam; PF05362; Lon_C; 1.
DR   PRINTS; PR00830; ENDOLAPTASE.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00464; LON; 1.
DR   TIGRFAMs; TIGR00763; lon; 1.
DR   PROSITE; PS01046; LON_SER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Cytoplasm; Hydrolase;
KW   Nucleotide-binding; Protease; Serine protease.
FT   CHAIN         1    778       ATP-dependent protease La.
FT                                /FTId=PRO_0000280893.
FT   DOMAIN        5    205       Lon.
FT   NP_BIND     356    363       ATP (Potential).
FT   ACT_SITE    679    679       By similarity.
FT   ACT_SITE    722    722       By similarity.
SQ   SEQUENCE   778 AA;  87027 MW;  E0855BF70147205A CRC64;
     MNKKSLPLMA LRDMVVFPGV IAPIFVGRQK SLQALSHTTV SEEDNSKYIL VTLQKKFDQE
     NPSKHELNNT AILAKIIQIV KLPNNTAKIL IEAVARVKLS NIKGNDAFEA NYEIIPDEEI
     FDVNNMRSLV DNAVQLFSKY AINDKKVNAE IIETINKEIS NSTNFINIIN ILASHLITSL
     EAKQHLLEET SPFKRITTVI STLTSNIVNS ETEQALQQRV RKQIEKTQRD YYLHEQMKAI
     QKELDEDKSE LADIEKKIKS LKLSKEAKEK AESELKKLRS MNQMSAESGV TRNYLETLLS
     LPWGKYDNSK IDINQAEKIL NRDHFGLEKV KERIIEYLAV LQRSSKIRGP ILCLIGPPGV
     GKTSLVKSIA EGMGRKYTKF ALGGVRDEAE IRGHRKTYLG STPGKILGQL KKVKTSNPVM
     LLDEIDKMSS DFRGDPASAL LEVLDPEQNS HFVDHYLEVE YDLSNVIFIA TANSHDLPRA
     LSDRMEKIYI SGYVEEEKLQ IAKNYLVPKQ FKMHKIKKDE ITISETAILD LIRYYTKESG
     VRALEREIGA LTRKALKQIL ADKTVKHIAI DSSNLEEFLE AKKYNFGLAE KEDQIGSTTG
     LAYTEVGGEL LTIEALAFPG KGEIKTTGKL GDVMKESAMA AYSCFRSRAT NFGLKYDNYK
     DFDIHIHVPA GAIPKDGPSA GCALFTTIVS LMTKIPVHRT VAMTGEITLR GNVLPIGGLK
     EKLLAASRGG IKTVLIPEEN VKDLKDIPPN IKESLEIISV SNIDQVLKHA LVETPINK
//