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Q4ULM3 (FOLD_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional protein FolD

Including the following 2 domains:

  1. Methylenetetrahydrofolate dehydrogenase
    EC=1.5.1.5
  2. Methenyltetrahydrofolate cyclohydrolase
    EC=3.5.4.9
Gene names
Name:folD
Ordered Locus Names:RF_0699
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate By similarity. HAMAP-Rule MF_01576

Catalytic activity

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH. HAMAP-Rule MF_01576

5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate. HAMAP-Rule MF_01576

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01576

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01576

Sequence similarities

Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

Sequence caution

The sequence AAY61550.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 291291Bifunctional protein FolD HAMAP-Rule MF_01576
PRO_0000268480

Regions

Nucleotide binding166 – 1683NADP By similarity

Sites

Binding site1911NADP By similarity
Binding site2321NADP; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4ULM3 [UniParc].

Last modified December 12, 2006. Version 2.
Checksum: 7B977E8835CF31DF

FASTA29131,230
        10         20         30         40         50         60 
MNNIIDGKAL ANEILADLKL EIQELKSQTN TSPKLAIVLV GDNPASIIYV KNKIKNAHKI 

        70         80         90        100        110        120 
GIDTLLVNLS TTIHTDDLIS KINELNLDNE ISGIIVQLPL PSSIDKNKIL SAVSPSKDID 

       130        140        150        160        170        180 
GFHPLNVGYL HSGISQGFIP CTALGCLAVI KKYEPNLTGK NVVIIGRSNI VGKPLSALLL 

       190        200        210        220        230        240 
KEDCSVTICH SKSQNLSSIT SKADIVVAAI GSPLKFTTEY FDPESIVIDV GINRISGNKI 

       250        260        270        280        290 
IGDVDFENVK SKVKYITPVP GGIGPMTIAF LLKNTVKAFK DSYSTVCHSC L 

« Hide

References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000053 Genomic DNA. Translation: AAY61550.1. Different initiation.
RefSeqYP_246715.1. NC_007109.1.

3D structure databases

ProteinModelPortalQ4ULM3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING315456.RF_0699.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY61550; AAY61550; RF_0699.
GeneID3400765.
KEGGrfe:RF_0699.
PATRIC17892024. VBIRicFel64634_0840.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0190.
HOGENOMHOG000218242.
KOK01491.
OMACTPYGIL.
OrthoDBEOG6K6VBB.
ProtClustDBPRK14171.

Enzyme and pathway databases

BioCycRFEL315456:GKEK-717-MONOMER.
UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_01576. THF_DHG_CYH.
InterProIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSPR00085. THFDHDRGNASE.
PROSITEPS00766. THF_DHG_CYH_1. 1 hit.
PS00767. THF_DHG_CYH_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFOLD_RICFE
AccessionPrimary (citable) accession number: Q4ULM3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 12, 2006
Last modified: February 19, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways