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Q4ULJ9

- TRUB_RICFE

UniProt

Q4ULJ9 - TRUB_RICFE

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Protein
tRNA pseudouridine synthase B
Gene
truB, RF_0723
Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs By similarity.UniRule annotation

Catalytic activityi

tRNA uridine(55) = tRNA pseudouridine(55).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei40 – 401Nucleophile By similarity

GO - Molecular functioni

  1. RNA binding Source: InterPro
  2. pseudouridine synthase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. tRNA pseudouridine synthesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-741-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase B (EC:5.4.99.25)
Alternative name(s):
tRNA pseudouridine(55) synthase
Short name:
Psi55 synthase
tRNA pseudouridylate synthase
tRNA-uridine isomerase
Gene namesi
Name:truB
Ordered Locus Names:RF_0723
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000008548: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 339339tRNA pseudouridine synthase BUniRule annotation
PRO_0000229379Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi315456.RF_0723.

Structurei

3D structure databases

ProteinModelPortaliQ4ULJ9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini262 – 30746RPE1 insert
Add
BLAST

Sequence similaritiesi

Contains 1 RPE1 insert domain.

Phylogenomic databases

eggNOGiCOG0130.
HOGENOMiHOG000231225.
KOiK03177.
OMAiEEAYTVC.
OrthoDBiEOG6358D3.

Family and domain databases

HAMAPiMF_01080. TruB_bact.
InterProiIPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR005728. Rickett_RPE.
IPR014780. tRNA_psdUridine_synth_TruB.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiPF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR01045. RPE1. 1 hit.
TIGR00431. TruB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4ULJ9-1 [UniParc]FASTAAdd to Basket

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MNSYWLNIYK PRGISSAKLV SMVKKILGKE VKVGHAGTLD VEAEGILPLA    50
VGEATKLIQL LIDAKKTYIF TVKFGTKTDS GDYAGTVIAT KDYIPSQEEA 100
YNVCSKFIGN VTQIPPAFSA LKVNGVRAYK LAREGKEVEL KPRNITIYNL 150
KCLNFDEKNA TASYYTECSK GTYIRTLAED LALSLQSLGF VIELRRTQVG 200
IFKEENAIRI KSPDEITKNF LEEKSIKIEA ILDDILVLDA TDSQAQQIKY 250
GQKCLFNYEE DFRRLSKFAY REEFEENTER STAAYTLVRE DANTGLTYKL 300
PLEVELSVSL LWVRYKGNLL AIGSLNKSCF NSLRVFNLL 339
Length:339
Mass (Da):38,192
Last modified:February 20, 2007 - v2
Checksum:iDCFEFC3AB19590E6
GO

Sequence cautioni

The sequence AAY61574.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000053 Genomic DNA. Translation: AAY61574.1. Different initiation.
RefSeqiYP_246739.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY61574; AAY61574; RF_0723.
GeneIDi3401097.
KEGGirfe:RF_0723.
PATRICi17892086. VBIRicFel64634_0871.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000053 Genomic DNA. Translation: AAY61574.1 . Different initiation.
RefSeqi YP_246739.1. NC_007109.1.

3D structure databases

ProteinModelPortali Q4ULJ9.
ModBasei Search...

Protein-protein interaction databases

STRINGi 315456.RF_0723.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY61574 ; AAY61574 ; RF_0723 .
GeneIDi 3401097.
KEGGi rfe:RF_0723.
PATRICi 17892086. VBIRicFel64634_0871.

Phylogenomic databases

eggNOGi COG0130.
HOGENOMi HOG000231225.
KOi K03177.
OMAi EEAYTVC.
OrthoDBi EOG6358D3.

Enzyme and pathway databases

BioCyci RFEL315456:GKEK-741-MONOMER.

Family and domain databases

HAMAPi MF_01080. TruB_bact.
InterProi IPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR005728. Rickett_RPE.
IPR014780. tRNA_psdUridine_synth_TruB.
[Graphical view ]
PANTHERi PTHR13767. PTHR13767. 1 hit.
Pfami PF01509. TruB_N. 1 hit.
[Graphical view ]
SUPFAMi SSF55120. SSF55120. 1 hit.
TIGRFAMsi TIGR01045. RPE1. 1 hit.
TIGR00431. TruB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.

Entry informationi

Entry nameiTRUB_RICFE
AccessioniPrimary (citable) accession number: Q4ULJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: February 20, 2007
Last modified: May 14, 2014
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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