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Q4ULJ9 (TRUB_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA pseudouridine synthase B

EC=5.4.99.25
Alternative name(s):
tRNA pseudouridine(55) synthase
Short name=Psi55 synthase
tRNA pseudouridylate synthase
tRNA-uridine isomerase
Gene names
Name:truB
Ordered Locus Names:RF_0723
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length339 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs By similarity. HAMAP-Rule MF_01080

Catalytic activity

tRNA uridine55 = tRNA pseudouridine55. HAMAP-Rule MF_01080

Sequence similarities

Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.

Contains 1 RPE1 insert domain.

Sequence caution

The sequence AAY61574.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA pseudouridine synthesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

pseudouridine synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 339339tRNA pseudouridine synthase B HAMAP-Rule MF_01080
PRO_0000229379

Regions

Domain262 – 30746RPE1 insert

Sites

Active site401Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4ULJ9 [UniParc].

Last modified February 20, 2007. Version 2.
Checksum: DCFEFC3AB19590E6

FASTA33938,192
        10         20         30         40         50         60 
MNSYWLNIYK PRGISSAKLV SMVKKILGKE VKVGHAGTLD VEAEGILPLA VGEATKLIQL 

        70         80         90        100        110        120 
LIDAKKTYIF TVKFGTKTDS GDYAGTVIAT KDYIPSQEEA YNVCSKFIGN VTQIPPAFSA 

       130        140        150        160        170        180 
LKVNGVRAYK LAREGKEVEL KPRNITIYNL KCLNFDEKNA TASYYTECSK GTYIRTLAED 

       190        200        210        220        230        240 
LALSLQSLGF VIELRRTQVG IFKEENAIRI KSPDEITKNF LEEKSIKIEA ILDDILVLDA 

       250        260        270        280        290        300 
TDSQAQQIKY GQKCLFNYEE DFRRLSKFAY REEFEENTER STAAYTLVRE DANTGLTYKL 

       310        320        330 
PLEVELSVSL LWVRYKGNLL AIGSLNKSCF NSLRVFNLL 

« Hide

References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000053 Genomic DNA. Translation: AAY61574.1. Different initiation.
RefSeqYP_246739.1. NC_007109.1.

3D structure databases

ProteinModelPortalQ4ULJ9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING315456.RF_0723.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY61574; AAY61574; RF_0723.
GeneID3401097.
KEGGrfe:RF_0723.
PATRIC17892086. VBIRicFel64634_0871.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0130.
HOGENOMHOG000231225.
KOK03177.
OMAEEAYTVC.
OrthoDBEOG6358D3.

Enzyme and pathway databases

BioCycRFEL315456:GKEK-741-MONOMER.

Family and domain databases

HAMAPMF_01080. TruB_bact.
InterProIPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR005728. Rickett_RPE.
IPR014780. tRNA_psdUridine_synth_TruB.
[Graphical view]
PANTHERPTHR13767. PTHR13767. 1 hit.
PfamPF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMSSF55120. SSF55120. 1 hit.
TIGRFAMsTIGR01045. RPE1. 1 hit.
TIGR00431. TruB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRUB_RICFE
AccessionPrimary (citable) accession number: Q4ULJ9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: February 20, 2007
Last modified: May 14, 2014
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names