Q4UL32 (FAD3_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative fatty acid oxidation complex trifunctional enzyme

Including the following 2 domains:

1. 3-hydroxyacyl-CoA dehydrogenase
   EC=1.1.1.35
2. Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase
   EC=4.2.1.17
   EC=5.3.3.8

Gene names

Ordered Locus Names:

RF_0890

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	720 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH. (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O. (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.
Sequence similarities	In the N-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family. In the C-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Ligand	NAD
Molecular function	Isomerase Lyase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	3-hydroxyacyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: EC coenzyme binding Inferred from electronic annotation. Source: InterPro dodecenoyl-CoA delta-isomerase activity Inferred from electronic annotation. Source: EC enoyl-CoA hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 720	720	Putative fatty acid oxidation complex trifunctional enzyme		PRO_0000285697
Regions
	Region	1 – 384	384	3-hydroxyacyl-CoA dehydrogenase
	Region	453 – 720	268	Enoyl-CoA hydratase/isomerase

Sequences

	Sequence		Length	Mass (Da)
	Q4UL32 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: AB2E8CBC1C1F1426	FASTA	720	81,043
	10 20 30 40 50 60 MQNEIKKVCV IGSGVMGSGI AALIANSSHK VVLLDIIAKD SDDPNKIVKT AKENLHKQKP 70 80 90 100 110 120 PPLSFPDKVN FITIGNLEHD LELIRDCDLV IEVIVEKLEI KHQLYNKIIP YLKEDAIIAS 130 140 150 160 170 180 NTSTLPLKRL KENLPDNIKS RFVITHFFNP PRYMELLELI IDSMVKFEVI ERVSGFLTKT 190 200 210 220 230 240 LGKTIVKCND TPGFIANRVG CFLLELVARK AISQKLDFVA LDKIFTICLG LPSTGIFGLY 250 260 270 280 290 300 DLIGHDVMKL ISSSLLAALP ANDAYHKIYV NTPVLDKMIE KKLIGRKGEG GFYRLSVSNG 310 320 330 340 350 360 KKIKEVININ DLSYSSVQKV DISFNNLDEL LVSDSVYGKI FTEIITEFYV YLASLVPSVT 370 380 390 400 410 420 DNIYDIDAAM KLGYSWKYGP FELLTIAAKD GWNSVIKNAD LMHIPLPQYL GNKEYQKIDK 430 440 450 460 470 480 QKFNSHKDIL QESQIVLEND SAKLINYREN FIFVITTKMN CLNHNVFYLL QEAASKAEKD 490 500 510 520 530 540 GKNLYIYPQG NNFSAGADLK LLLSYIEDGN FHDLENLLKL GQQTMLHLKY SGVHIISCAK 550 560 570 580 590 600 GVALGGGCEL LLYSSYIVAN QELNAGLVEL GVGLIPGWGG VTEMFVRSKG DKTKLIRNVR 610 620 630 640 650 660 NIIEQNKTSS ADYFKSDYDV ESMHVNMNKH YILDEALKLN LSKKIVPIPH KITLPKINLA 670 680 690 700 710 720 TEIDTSKYND LQNKVLSKFQ NIIDKHPDTN EEELLGYERE IFLELAKDPK TIEKLKAIVK

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY61741.1.
RefSeq	YP_246906.1. NC_007109.1.
3D structure databases
ProteinModelPortal	Q4UL32.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_0890.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY61741; AAY61741; RF_0890.
GeneID	3401556.
KEGG	rfe:RF_0890.
PATRIC	17892493. VBIRicFel64634_1068.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1250.
HOGENOM	HOG000264370.
KO	K01782.
OMA	GQGFYKK.
ProtClustDB	CLSK870987.
Enzyme and pathway databases
BioCyc	RFEL315456:GKEK-915-MONOMER.
Family and domain databases
Gene3D	1.10.1040.10. 1 hit. 3.40.50.720. 1 hit.
InterPro	IPR006176. 3-OHacyl-CoA_DH_NAD-bd. IPR006108. 3HC_DH_C. IPR008927. 6-PGluconate_DH_C-like. IPR001753. Crotonase_core_superfam. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF00725. 3HCDH. 1 hit. PF02737. 3HCDH_N. 1 hit. PF00378. ECH. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 2 hits.
PROSITE	PS00067. 3HCDH. False negative. PS00166. ENOYL_COA_HYDRATASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FAD3_RICFE

Accession

Primary (citable) accession number: Q4UL32

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	July 5, 2005
Last modified:	May 29, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program