Q4UKW1 (CCME_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c-type biogenesis protein CcmE

Alternative name(s):
Cytochrome c maturation protein E
Heme chaperone CcmE

Gene names

Name:	ccmE
Synonyms:	cycJ
Ordered Locus Names:	RF_0961

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	128 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by CcmC and transfers the heme to apo-cytochromes in a process facilitated by CcmF and CcmH By similarity. HAMAP-Rule MF_01959
Subcellular location	Cell inner membrane; Single-pass type II membrane protein; Periplasmic side Potential HAMAP-Rule MF_01959.
Sequence similarities	Belongs to the CcmE/CycJ family.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cytochrome complex assembly Inferred from electronic annotation. Source: UniProtKB-KW protein-heme linkage Inferred from electronic annotation. Source: HAMAP
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 128	128	Cytochrome c-type biogenesis protein CcmE HAMAP-Rule MF_01959		PRO_0000238856
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 29	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	30 – 128	99	Periplasmic Potential
Sites
	Metal binding	124	1	Iron (heme axial ligand) By similarity
	Binding site	120	1	Heme (covalent; via pros nitrogen) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UKW1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 9F96E756A87B6677	FASTA	128	14,351
	10 20 30 40 50 60 MQKRVRNRLI TIIICFCSAA LGISIVLYNL EKNIVFFLPP SKINEIEQGK ELRVGGLVKI 70 80 90 100 110 120 DSINKIAADK ISFVITDNIR DLEILYQGVL PALFREGQGI IAIGQLSDGK FIARQLLAKH DENYRPPS

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY61812.1.
RefSeq	YP_246977.1. NC_007109.1.
3D structure databases
ProteinModelPortal	Q4UKW1.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_0961.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY61812; AAY61812; RF_0961.
GeneID	3400969.
KEGG	rfe:RF_0961.
PATRIC	17892669. VBIRicFel64634_1152.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2332.
HOGENOM	HOG000009661.
KO	K02197.
OMA	ICFCSAC.
ProtClustDB	PRK13254.
Enzyme and pathway databases
BioCyc	RFEL315456:GKEK-990-MONOMER.
Family and domain databases
Gene3D	2.40.50.140. 1 hit.
HAMAP	MF_01959. CcmE.
InterPro	IPR004329. CcmE. IPR012340. NA-bd_OB-fold. [Graphical view]
Pfam	PF03100. CcmE. 1 hit. [Graphical view]
SUPFAM	SSF82093. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CCME_RICFE

Accession

Primary (citable) accession number: Q4UKW1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	July 5, 2005
Last modified:	May 29, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program