Q4UKW0 (IPYR_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inorganic pyrophosphatase
EC=3.6.1.1

Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name=PPase

Gene names

Name:	ppa
Ordered Locus Names:	RF_0962

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	173 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP-Rule MF_00209
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PPase family.
Sequence caution	The sequence AAY61813.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	phosphate-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 173	173	Inorganic pyrophosphatase HAMAP-Rule MF_00209		PRO_0000278036
Sites
	Metal binding	65	1	Magnesium 1 By similarity
	Metal binding	70	1	Magnesium 1 By similarity
	Metal binding	70	1	Magnesium 2 By similarity
	Metal binding	102	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UKW0 [UniParc]. Last modified February 20, 2007. Version 2. Checksum: 50C73DDA61C42B4B	FASTA	173	19,551
	10 20 30 40 50 60 MFIDKIKAKA NNDEINVIIE IPMNIGPIKY EFDKESGAVF VDRFMQTTMS YPCNYGFIPH 70 80 90 100 110 120 TLSNDGDPVD VLVVAHHPVV PGSVIKCRAV GVLMMEDESG LDEKIIAVPT SKLDITFDHI 130 140 150 160 170 KELDDLCEML KKRIVHFFEH YKDLEKGKWV KVTGWENKAK ANALINEGID RAS

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY61813.1. Different initiation.
RefSeq	YP_246978.2. NC_007109.1.
3D structure databases
HSSP	HSSP built from PDB template 1FAJ based on UniProtKB P0A7A9.
ProteinModelPortal	Q4UKW0.
SMR	Q4UKW0. Positions 2-171.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_0962.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY61813; AAY61813; RF_0962.
GeneID	3400970.
KEGG	rfe:RF_0962.
PATRIC	17892671. VBIRicFel64634_1153.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0221.
HOGENOM	HOG000236473.
KO	K01507.
OMA	WNRELAV.
ProtClustDB	PRK01250.
Family and domain databases
Gene3D	3.90.80.10. 1 hit.
HAMAP	MF_00209. Inorganic_PPase.
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
PANTHER	PTHR10286. PTHR10286. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
SUPFAM	SSF50324. Pyrophosphatase. 1 hit.
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_RICFE

Accession

Primary (citable) accession number: Q4UKW0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	February 20, 2007
Last modified:	May 1, 2013
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program