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Q4UKW0 (IPYR_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inorganic pyrophosphatase

EC=3.6.1.1
Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name=PPase
Gene names
Name:ppa
Ordered Locus Names:RF_0962
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length173 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Diphosphate + H2O = 2 phosphate. HAMAP-Rule MF_00209

Cofactor

Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00209.

Sequence similarities

Belongs to the PPase family.

Sequence caution

The sequence AAY61813.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMagnesium
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processphosphate-containing compound metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioninorganic diphosphatase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 173173Inorganic pyrophosphatase HAMAP-Rule MF_00209
PRO_0000278036

Sites

Metal binding651Magnesium 1 By similarity
Metal binding701Magnesium 1 By similarity
Metal binding701Magnesium 2 By similarity
Metal binding1021Magnesium 1 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4UKW0 [UniParc].

Last modified February 20, 2007. Version 2.
Checksum: 50C73DDA61C42B4B

FASTA17319,551
        10         20         30         40         50         60 
MFIDKIKAKA NNDEINVIIE IPMNIGPIKY EFDKESGAVF VDRFMQTTMS YPCNYGFIPH 

        70         80         90        100        110        120 
TLSNDGDPVD VLVVAHHPVV PGSVIKCRAV GVLMMEDESG LDEKIIAVPT SKLDITFDHI 

       130        140        150        160        170 
KELDDLCEML KKRIVHFFEH YKDLEKGKWV KVTGWENKAK ANALINEGID RAS 

« Hide

References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000053 Genomic DNA. Translation: AAY61813.1. Different initiation.
RefSeqYP_246978.2. NC_007109.1.

3D structure databases

ProteinModelPortalQ4UKW0.
SMRQ4UKW0. Positions 2-171.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING315456.RF_0962.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY61813; AAY61813; RF_0962.
GeneID3400970.
KEGGrfe:RF_0962.
PATRIC17892671. VBIRicFel64634_1153.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0221.
HOGENOMHOG000236473.
KOK01507.
OMAGILHMTD.
OrthoDBEOG6NKR4X.
ProtClustDBPRK01250.

Enzyme and pathway databases

BioCycRFEL315456:GKEK-991-MONOMER.

Family and domain databases

Gene3D3.90.80.10. 1 hit.
HAMAPMF_00209. Inorganic_PPase.
InterProIPR008162. Pyrophosphatase.
[Graphical view]
PANTHERPTHR10286. PTHR10286. 1 hit.
PfamPF00719. Pyrophosphatase. 1 hit.
[Graphical view]
SUPFAMSSF50324. SSF50324. 1 hit.
PROSITEPS00387. PPASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIPYR_RICFE
AccessionPrimary (citable) accession number: Q4UKW0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 20, 2007
Last modified: February 19, 2014
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names