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Q4UKU3 (SYT_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Threonine--tRNA ligase

EC=6.1.1.3
Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS
Gene names
Name:thrS
Ordered Locus Names:RF_0979
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length635 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP-Rule MF_00184

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00184

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00184

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00184.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processthreonyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

threonine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 635635Threonine--tRNA ligase HAMAP-Rule MF_00184
PRO_0000101037

Regions

Region242 – 533292Catalytic HAMAP-Rule MF_00184

Sites

Metal binding3331Zinc; catalytic By similarity
Metal binding3841Zinc; catalytic By similarity
Metal binding5101Zinc; catalytic By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4UKU3 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: 3B11869BA3758CB3

FASTA63572,589
        10         20         30         40         50         60 
MINISFPDGS VKQFESNITA FEVANAISMS LAKAAMVAEI NGELKDLSTI IENDCKLRIL 

        70         80         90        100        110        120 
TAKDSECLEI IRHDAAHIIA EAAKELFPDI QVTIGPAIEN GFFYDFAKDK PFTPDDVAMI 

       130        140        150        160        170        180 
EARMHEIVKR NEQITRELWD RDKAVEFFKS IGEHYKAEII ASIPAGEPIT LYRQGSFIDL 

       190        200        210        220        230        240 
CRGPHAPSTG FVKHFKLMKV AGAYWRGDSR NEMLQRIYGT AWATKEQLDN YLFMLEEAEK 

       250        260        270        280        290        300 
RDHRKLGREL DLFHFQEEAQ GMVFWHDKGW SIYNTIEQYI RKKIRKNGYT EVKTPVLVDK 

       310        320        330        340        350        360 
SLWEASGHWE KFREDMFALE TDDKTLALKP MNCPCHVQIF KQGIKSYRDL PLRMSEFGLC 

       370        380        390        400        410        420 
HRNEASGALH GLMRVRSLVQ DDAHIFCAEE QITDETVSFC KLLTEVYKDF GFTDIKVKFS 

       430        440        450        460        470        480 
DRPEIRAGSD EVWDKAENAL KEAVEKAGFT YTLNPGEGAF YGPKLEFVLT DAIGRQWQCG 

       490        500        510        520        530        540 
TLQMDFVLPE RLDASYIAAS GEKKRPVMLH RAILGSLERF IGILIEEYAG RFPIWLAPIQ 

       550        560        570        580        590        600 
VAIATITSDL NDYALEVQKA LIDNGVRTDI NISPDKINYK IREFSNQKIP MIAVIGKQEK 

       610        620        630 
ENKHVTIRRL GTTDQEVLSI EQLIALIKKE NEKYL 

« Hide

References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000053 Genomic DNA. Translation: AAY61830.1.
RefSeqYP_246995.1. NC_007109.1.

3D structure databases

ProteinModelPortalQ4UKU3.
SMRQ4UKU3. Positions 241-631.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING315456.RF_0979.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY61830; AAY61830; RF_0979.
GeneID3400987.
KEGGrfe:RF_0979.
PATRIC17892707. VBIRicFel64634_1171.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0441.
HOGENOMHOG000003880.
KOK01868.
OMACVGHIKV.
OrthoDBEOG61KBFJ.
ProtClustDBPRK00413.

Enzyme and pathway databases

BioCycRFEL315456:GKEK-1008-MONOMER.

Family and domain databases

Gene3D3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPMF_00184. Thr_tRNA_synth.
InterProIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR012675. Beta-grasp_dom.
IPR004095. TGS.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
PfamPF03129. HGTP_anticodon. 1 hit.
PF02824. TGS. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSPR01047. TRNASYNTHTHR.
SMARTSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsTIGR00418. thrS. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYT_RICFE
AccessionPrimary (citable) accession number: Q4UKU3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: July 5, 2005
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries