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Protein

Threonine--tRNA ligase

Gene

thrS

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi333 – 3331Zinc; catalyticUniRule annotation
Metal bindingi384 – 3841Zinc; catalyticUniRule annotation
Metal bindingi510 – 5101Zinc; catalyticUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-1008-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligaseUniRule annotation (EC:6.1.1.3UniRule annotation)
Alternative name(s):
Threonyl-tRNA synthetaseUniRule annotation
Short name:
ThrRSUniRule annotation
Gene namesi
Name:thrSUniRule annotation
Ordered Locus Names:RF_0979
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000008548 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 635635Threonine--tRNA ligasePRO_0000101037Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi315456.RF_0979.

Structurei

3D structure databases

ProteinModelPortaliQ4UKU3.
SMRiQ4UKU3. Positions 241-631.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni242 – 533292CatalyticAdd
BLAST

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0441.
HOGENOMiHOG000003880.
KOiK01868.
OMAiKSGHWDK.
OrthoDBiEOG61KBFJ.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR012675. Beta-grasp_dom.
IPR004095. TGS.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF02824. TGS. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4UKU3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINISFPDGS VKQFESNITA FEVANAISMS LAKAAMVAEI NGELKDLSTI
60 70 80 90 100
IENDCKLRIL TAKDSECLEI IRHDAAHIIA EAAKELFPDI QVTIGPAIEN
110 120 130 140 150
GFFYDFAKDK PFTPDDVAMI EARMHEIVKR NEQITRELWD RDKAVEFFKS
160 170 180 190 200
IGEHYKAEII ASIPAGEPIT LYRQGSFIDL CRGPHAPSTG FVKHFKLMKV
210 220 230 240 250
AGAYWRGDSR NEMLQRIYGT AWATKEQLDN YLFMLEEAEK RDHRKLGREL
260 270 280 290 300
DLFHFQEEAQ GMVFWHDKGW SIYNTIEQYI RKKIRKNGYT EVKTPVLVDK
310 320 330 340 350
SLWEASGHWE KFREDMFALE TDDKTLALKP MNCPCHVQIF KQGIKSYRDL
360 370 380 390 400
PLRMSEFGLC HRNEASGALH GLMRVRSLVQ DDAHIFCAEE QITDETVSFC
410 420 430 440 450
KLLTEVYKDF GFTDIKVKFS DRPEIRAGSD EVWDKAENAL KEAVEKAGFT
460 470 480 490 500
YTLNPGEGAF YGPKLEFVLT DAIGRQWQCG TLQMDFVLPE RLDASYIAAS
510 520 530 540 550
GEKKRPVMLH RAILGSLERF IGILIEEYAG RFPIWLAPIQ VAIATITSDL
560 570 580 590 600
NDYALEVQKA LIDNGVRTDI NISPDKINYK IREFSNQKIP MIAVIGKQEK
610 620 630
ENKHVTIRRL GTTDQEVLSI EQLIALIKKE NEKYL
Length:635
Mass (Da):72,589
Last modified:July 5, 2005 - v1
Checksum:i3B11869BA3758CB3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61830.1.
RefSeqiWP_011271297.1. NC_007109.1.
YP_246995.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY61830; AAY61830; RF_0979.
KEGGirfe:RF_0979.
PATRICi17892707. VBIRicFel64634_1171.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61830.1.
RefSeqiWP_011271297.1. NC_007109.1.
YP_246995.1. NC_007109.1.

3D structure databases

ProteinModelPortaliQ4UKU3.
SMRiQ4UKU3. Positions 241-631.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi315456.RF_0979.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY61830; AAY61830; RF_0979.
KEGGirfe:RF_0979.
PATRICi17892707. VBIRicFel64634_1171.

Phylogenomic databases

eggNOGiCOG0441.
HOGENOMiHOG000003880.
KOiK01868.
OMAiKSGHWDK.
OrthoDBiEOG61KBFJ.

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-1008-MONOMER.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR012675. Beta-grasp_dom.
IPR004095. TGS.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF02824. TGS. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.

Entry informationi

Entry nameiSYT_RICFE
AccessioniPrimary (citable) accession number: Q4UKU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: July 5, 2005
Last modified: April 1, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.