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Q4UKT4

- PARE_RICFE

UniProt

Q4UKT4 - PARE_RICFE

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Protein

DNA topoisomerase 4 subunit B

Gene

parE

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ and Ca2+.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei20 – 201ATPUniRule annotation
Binding sitei60 – 601ATPUniRule annotation
Binding sitei87 – 871ATPUniRule annotation
Binding sitei359 – 3591ATPUniRule annotation
Metal bindingi445 – 4451Magnesium 1; catalyticUniRule annotation
Sitei470 – 4701Interaction with DNAUniRule annotation
Sitei473 – 4731Interaction with DNAUniRule annotation
Metal bindingi518 – 5181Magnesium 1; catalyticUniRule annotation
Metal bindingi518 – 5181Magnesium 2UniRule annotation
Metal bindingi520 – 5201Magnesium 2UniRule annotation
Sitei525 – 5251Interaction with DNAUniRule annotation
Sitei641 – 6411Interaction with DNAUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi129 – 1357ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: UniProtKB-HAMAP
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. chromosome segregation Source: UniProtKB-HAMAP
  2. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-1017-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 4 subunit BUniRule annotation (EC:5.99.1.3UniRule annotation)
Alternative name(s):
Topoisomerase IV subunit BUniRule annotation
Gene namesi
Name:parEUniRule annotation
Ordered Locus Names:RF_0988
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000008548: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 662662DNA topoisomerase 4 subunit BPRO_0000273118Add
BLAST

Interactioni

Subunit structurei

Heterotetramer composed of ParC and ParE.UniRule annotation

Protein-protein interaction databases

STRINGi315456.RF_0988.

Structurei

3D structure databases

ProteinModelPortaliQ4UKT4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini439 – 553115ToprimUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the type II topoisomerase family. ParE type 1 subfamily.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0187.
HOGENOMiHOG000075154.
KOiK02622.
OMAiICEEASK.
OrthoDBiEOG6P334W.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPiMF_00938. ParE_type1.
InterProiIPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR005737. TopoIV_B_Gneg.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01055. parE_Gneg. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4UKT4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDLFSFNKE KKNKLVDNNY SAKDIEVLEG LEPVRKRPGM YIGGTDSNAM
60 70 80 90 100
HHLVSEVLDN AMDEAVAGFA SIITIKMHQD HSITIFDNGR GIPIDNHPKF
110 120 130 140 150
PDKSALEVIL TTLHSGGKFS NNVYHTAGGL HGVGISVVNA LSKHLEIKVY
160 170 180 190 200
KQGKLYSQSY SKGEKLTDLI CEEASKRLRG TSINFTPDPE IFSEKLHFNP
210 220 230 240 250
KKIYELARSK AYLYRGVTIE WECEVEVPSD IPKKALINFP NGLKDYLSSK
260 270 280 290 300
ITLDNLIIPE IFSGNIESTP DEIKLEWAIC WQNNDNSAFI QSYCNTVPTP
310 320 330 340 350
QGGTHEQGLK SAILRGLKAY GEMIGNKKAA NLTIEDILET ASVVLSIFIA
360 370 380 390 400
EPSFQGQTKE KLVSNGVSKP VENIIKDHFD HFLSSDKALA TNLLEHVIAI
410 420 430 440 450
AEFRISKKNE KNISRKNATQ KLRLPGKLAD CTRTTPGGTE LFIVEGDSAG
460 470 480 490 500
GSAKQARNRE TQAVLPLWGK VLNVASSTLE KIVNNQAIQD LEIALACGSL
510 520 530 540 550
KNYKEENLRY EKIIIMTDAD VDGAHIASLL MTFFFLRMPK LVEEGHLYLA
560 570 580 590 600
KPPLYRLTQS NKTYYAGDEE EKAKLTDKLS KASKAKIEVG RFKGLGEMMP
610 620 630 640 650
AQLKETTMHP EKRSLLKVTL EDFQNVDKIV DDLMGKKPEK RFQFIYEQAL
660
VKMDKIINEL DI
Length:662
Mass (Da):73,954
Last modified:July 5, 2005 - v1
Checksum:i7D80C19453C7F0D7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000053 Genomic DNA. Translation: AAY61839.1.
RefSeqiYP_247004.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY61839; AAY61839; RF_0988.
GeneIDi3400996.
KEGGirfe:RF_0988.
PATRICi17892727. VBIRicFel64634_1181.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000053 Genomic DNA. Translation: AAY61839.1 .
RefSeqi YP_247004.1. NC_007109.1.

3D structure databases

ProteinModelPortali Q4UKT4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 315456.RF_0988.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY61839 ; AAY61839 ; RF_0988 .
GeneIDi 3400996.
KEGGi rfe:RF_0988.
PATRICi 17892727. VBIRicFel64634_1181.

Phylogenomic databases

eggNOGi COG0187.
HOGENOMi HOG000075154.
KOi K02622.
OMAi ICEEASK.
OrthoDBi EOG6P334W.

Enzyme and pathway databases

BioCyci RFEL315456:GKEK-1017-MONOMER.

Family and domain databases

Gene3Di 3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPi MF_00938. ParE_type1.
InterProi IPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR005737. TopoIV_B_Gneg.
IPR006171. Toprim_domain.
[Graphical view ]
Pfami PF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00418. TPI2FAMILY.
SMARTi SM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view ]
SUPFAMi SSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsi TIGR01055. parE_Gneg. 1 hit.
PROSITEi PS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.

Entry informationi

Entry nameiPARE_RICFE
AccessioniPrimary (citable) accession number: Q4UKT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: July 5, 2005
Last modified: October 29, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3