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Q4UKT4 (PARE_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA topoisomerase 4 subunit B
EC=5.99.1.3
Alternative name(s):
Topoisomerase IV subunit B
Gene names
Name:parE
Ordered Locus Names:RF_0988
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group ›

Protein attributes

Sequence length662 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule By similarity. HAMAP-Rule MF_00938

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_00938

Cofactor

Magnesium. Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ and Ca2+ By similarity.

Subunit structure

Heterotetramer composed of ParC and ParE By similarity.

Sequence similarities

Belongs to the type II topoisomerase family. ParE type 1 subfamily.

Contains 1 Toprim domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 662662DNA topoisomerase 4 subunit B HAMAP-Rule MF_00938
PRO_0000273118

Regions

Domain439 – 553115Toprim
Nucleotide binding129 – 1357ATP By similarity

Sites

Metal binding4451Magnesium 1; catalytic By similarity
Metal binding5181Magnesium 1; catalytic By similarity
Metal binding5181Magnesium 2 By similarity
Metal binding5201Magnesium 2 By similarity
Binding site201ATP By similarity
Binding site601ATP By similarity
Binding site871ATP By similarity
Binding site3591ATP By similarity
Site4701Interaction with DNA By similarity
Site4731Interaction with DNA By similarity
Site5251Interaction with DNA By similarity
Site6411Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4UKT4 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: 7D80C19453C7F0D7

FASTA66273,954
        10         20         30         40         50         60 
MSDLFSFNKE KKNKLVDNNY SAKDIEVLEG LEPVRKRPGM YIGGTDSNAM HHLVSEVLDN 

        70         80         90        100        110        120 
AMDEAVAGFA SIITIKMHQD HSITIFDNGR GIPIDNHPKF PDKSALEVIL TTLHSGGKFS 

       130        140        150        160        170        180 
NNVYHTAGGL HGVGISVVNA LSKHLEIKVY KQGKLYSQSY SKGEKLTDLI CEEASKRLRG 

       190        200        210        220        230        240 
TSINFTPDPE IFSEKLHFNP KKIYELARSK AYLYRGVTIE WECEVEVPSD IPKKALINFP 

       250        260        270        280        290        300 
NGLKDYLSSK ITLDNLIIPE IFSGNIESTP DEIKLEWAIC WQNNDNSAFI QSYCNTVPTP 

       310        320        330        340        350        360 
QGGTHEQGLK SAILRGLKAY GEMIGNKKAA NLTIEDILET ASVVLSIFIA EPSFQGQTKE 

       370        380        390        400        410        420 
KLVSNGVSKP VENIIKDHFD HFLSSDKALA TNLLEHVIAI AEFRISKKNE KNISRKNATQ 

       430        440        450        460        470        480 
KLRLPGKLAD CTRTTPGGTE LFIVEGDSAG GSAKQARNRE TQAVLPLWGK VLNVASSTLE 

       490        500        510        520        530        540 
KIVNNQAIQD LEIALACGSL KNYKEENLRY EKIIIMTDAD VDGAHIASLL MTFFFLRMPK 

       550        560        570        580        590        600 
LVEEGHLYLA KPPLYRLTQS NKTYYAGDEE EKAKLTDKLS KASKAKIEVG RFKGLGEMMP 

       610        620        630        640        650        660 
AQLKETTMHP EKRSLLKVTL EDFQNVDKIV DDLMGKKPEK RFQFIYEQAL VKMDKIINEL 


DI

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References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000053 Genomic DNA. Translation: AAY61839.1.
RefSeqYP_247004.1. NC_007109.1.

3D structure databases

HSSPHSSP built from PDB template 1S14 based on UniProtKB P20083.
ProteinModelPortalQ4UKT4.
ModBaseSearch...

Protein-protein interaction databases

STRING315456.RF_0988.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY61839; AAY61839; RF_0988.
GeneID3400996.
KEGGrfe:RF_0988.
PATRIC17892727. VBIRicFel64634_1181.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0187.
HOGENOMHOG000075154.
KOK02622.
OMAQARDKEF.
ProtClustDBPRK05559.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPMF_00938. ParE_type1.
InterProIPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR005737. TopoIV_B_Gneg.
IPR006171. Toprim_domain.
[Graphical view]
PfamPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00418. TPI2FAMILY.
SMARTSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMSSF55874. ATP_bd_ATPase. 1 hit.
SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMsTIGR01055. parE_Gneg. 1 hit.
PROSITEPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePARE_RICFE
AccessionPrimary (citable) accession number: Q4UKT4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: July 5, 2005
Last modified: May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents