Q4UKR6 (UCRI_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquinol-cytochrome c reductase iron-sulfur subunit
EC=1.10.2.2

Alternative name(s):
Rieske iron-sulfur protein
Short name=RISP

Gene names

Name:	petA
Ordered Locus Names:	RF_1010

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
Catalytic activity	QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H+.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The main subunits of complex b-c1 are: cytochrome b, cytochrome c1 and the Rieske protein.
Subcellular location	Cell membrane; Single-pass membrane protein Potential.
Miscellaneous	The Rieske protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 177	177	Ubiquinol-cytochrome c reductase iron-sulfur subunit		PRO_0000286643
Regions
	Transmembrane	18 – 38	21	Helical; Potential
	Domain	88 – 175	88	Rieske
Sites
	Metal binding	120	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	122	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	139	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	142	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	125 ↔ 141		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UKR6 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 8F500C77E8FD743D	FASTA	177	19,228
	10 20 30 40 50 60 MSDTEDNKNK QTTRRDFMVL TASSVAAVGA VCTLWPLVDS LNPSADVLAL SSIEVDLSNI 70 80 90 100 110 120 AVGQTVTVKW QGKPVFITNR TPDKIAEARA VKMSELIDPE TDEARVKAGH DNWLVTIGIC 130 140 150 160 170 THLGCVPLAN QGEYDGWFCP CHGSQYDSSG RVRRGPAPLN LAVPPYTFIS DKKIRIG

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY61861.1.
RefSeq	YP_247026.1. NC_007109.1.
3D structure databases
ProteinModelPortal	Q4UKR6.
SMR	Q4UKR6. Positions 118-163.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_1010.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY61861; AAY61861; RF_1010.
GeneID	3401020.
KEGG	rfe:RF_1010.
PATRIC	17892785. VBIRicFel64634_1208.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0723.
HOGENOM	HOG000255193.
KO	K00411.
OMA	WRSIKPE.
ProtClustDB	CLSK870834.
Enzyme and pathway databases
BioCyc	RFEL315456:GKEK-1042-MONOMER.
Family and domain databases
Gene3D	2.102.10.10. 1 hit.
InterPro	IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR005805. Rieske_Fe-S_prot_C. IPR006311. TAT_signal. IPR019546. TAT_signal_bac_arc. IPR019470. Ubiq_cytC_Rdtase_Fe-S_su_TAT. IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su. [Graphical view]
PANTHER	PTHR10134. PTHR10134. 1 hit.
Pfam	PF00355. Rieske. 1 hit. PF10399. UCR_Fe-S_N. 1 hit. [Graphical view]
PRINTS	PR00162. RIESKE.
SUPFAM	SSF50022. Rieske_dom. 1 hit.
TIGRFAMs	TIGR01416. Rieske_proteo. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRI_RICFE

Accession

Primary (citable) accession number: Q4UKR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	July 5, 2005
Last modified:	May 29, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program