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Q4UKP1 (DDL_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:RF_1035
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length321 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 321321D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000277918

Regions

Domain121 – 315195ATP-grasp
Nucleotide binding147 – 19953ATP By similarity

Sites

Metal binding2681Magnesium or manganese 1 By similarity
Metal binding2821Magnesium or manganese 1 By similarity
Metal binding2821Magnesium or manganese 2 By similarity
Metal binding2841Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4UKP1 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: 6F91460A67161BE8

FASTA32135,914
        10         20         30         40         50         60 
MNKYQTHWVE HSEVKILSTT GKKHIALVAG GMSAEREVSL VSSEGVSKAL IELGYKVTFI 

        70         80         90        100        110        120 
DMGADIAVKL QEIKPDIVFN CLHGTYGEDG CLPGLLNIMR IPYTHSGVLS SALAFDKIHS 

       130        140        150        160        170        180 
RSWFLTNNIN MAESIIVNKS DNIKSDPVKR PYVIKPLTQG SSIGVEVIFE EDDFNFADYN 

       190        200        210        220        230        240 
FPYGYQVIIE QYIKGRELQV AVLNGKALGA LEIKLLKNRF YDYETKYTEG FAEHLCPAPL 

       250        260        270        280        290        300 
PTNLYEKLLV ESEKIYKTMN CKGPARAEFI LEEQTNKLYA LEINTHPGMT PLSIVPEIAA 

       310        320 
YAGINFTNLI EEIIKTASFE S 

« Hide

References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000053 Genomic DNA. Translation: AAY61886.1.
RefSeqYP_247051.1. NC_007109.1.

3D structure databases

ProteinModelPortalQ4UKP1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING315456.RF_1035.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY61886; AAY61886; RF_1035.
GeneID3400145.
KEGGrfe:RF_1035.
PATRIC17892848. VBIRicFel64634_1239.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAKYTEGFA.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycRFEL315456:GKEK-1067-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_RICFE
AccessionPrimary (citable) accession number: Q4UKP1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: July 5, 2005
Last modified: February 19, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways