Q4UKM1 (GYRA_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	RF_1055

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	906 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 906	906	DNA gyrase subunit A HAMAP MF_01897		PRO_0000273108
Sites
	Active site	123	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UKM1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 20FE68CE7B0F8FB0	FASTA	906	101,121
	10 20 30 40 50 60 MTDQNFSNLV PVNIEDEMKV SYLDYAMSVI VSRAIPDVRD GLKPVHRRII YSMYEAGNHA 70 80 90 100 110 120 SKPYRKSARI VGDVMGKYHP HGDSAIYDSL VRMAQDFSLR LPLVDGQGNF GSMDGDAAAA 130 140 150 160 170 180 MRYTESRMAK VSHKLVEDID KETVSFNPNY DGSEEEPSVL PAMFPNLLVN GSGGIAVGMA 190 200 210 220 230 240 TNIPPHNLGE VIDACCLYID NNDIEILDLL EVVKGPDFPT GSMILGISGI RSAYLTGRGS 250 260 270 280 290 300 IIMRGRAEIE NIGNSRQAII ITEIPYMVNK ARLVEKIAEM VKEKRIEGIS DLRDESNKNG 310 320 330 340 350 360 VRIFIELKKD VVVEVVLNQI YACTQLQTSF GVIMLALKDG LPKVMNLKEV IAAFVSFREV 370 380 390 400 410 420 VITNRTIYLL NKARDRAHIL LGLTIAVSNI DEIIRIIKAS NNPNAAKQEL MARSWDALNI 430 440 450 460 470 480 LPLVKLVDDK AMLNEQGKCS FTEVQAKAIL EMRLQRLTAM EKNKLEEDLK NLATEITEYL 490 500 510 520 530 540 NILGSRTRLL EILKEELIKV KEEFATPRLT SIEFGEFDQD IEDLIQREEM VVTVTLGGYI 550 560 570 580 590 600 KRVPLSSYRA QKRGGKGRSG LSMRDEDITT QVFVGSTHTP MLFFSNIGQV YSLKLYKLPL 610 620 630 640 650 660 SNPQGKGRPM VNILPLKENE HITNIMPLPE NQDEWDNLNI MFATAKGNIR RSDLLDFKKI 670 680 690 700 710 720 QSNGKIAIRL DEDDKLIDVK PCKEDEHILL ATKAGKALRF PVESLRVIKS RTSDGVRGMK 730 740 750 760 770 780 LAKEDSVISM TVLKGISSTK EDRDAYLTVP WEKRLEIAKG EEFNLEELGV TLTADSILEM 790 800 810 820 830 840 ANSEEFILTV TENGFGKRSS AYGYRITDRG GSGIINMDIN DKTGLVVGVM PVKMDDELML 850 860 870 880 890 900 ITNSGKLIRC KLESVRITGR NTSGVILFKL DDGEKVVSVS LIAETSESEE DSELEEDLEQ AEEVYT

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY61906.1.
RefSeq	YP_247071.1. NC_007109.1.
3D structure databases
HSSP	HSSP built from PDB template 1SUU based on UniProtKB O51396.
ProteinModelPortal	Q4UKM1.
SMR	Q4UKM1. Positions 31-512.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3400165.
GenomeReviews	Gene locus RF_1055 in contig CP000053_GR.
KEGG	rfe:RF_1055.
NMPDR	fig|315456.3.peg.1055.
PATRIC	17892896. VBIRicFel64634_1263.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG635183.
OMA	EEYSAQG.
ProtClustDB	PRK05560.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_1055-MONOMER.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_RICFE

Accession

Primary (citable) accession number: Q4UKM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	July 5, 2005
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program