Reviewed, UniProtKB/Swiss-Prot Q4UKI8 (ODO1_RICFE)

Names and origin

Protein names

Recommended name:
    2-oxoglutarate dehydrogenase E1 component
    EC=1.2.4.2
Alternative name(s):
    Alpha-ketoglutarate dehydrogenase

Gene names

Name:	sucA
Ordered Locus Names:	RF_1092

Organism

Rickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

42862 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	977 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity.
Catalytic activity	2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2.
Cofactor	Thiamine pyrophosphate By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the alpha-ketoglutarate dehydrogenase family. Contains 1 RPE1 insert domain.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Thiamine pyrophosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxoglutarate dehydrogenase (succinyl-transferring) activity Inferred from electronic annotation. Source: EC thiamin pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 977	977	2-oxoglutarate dehydrogenase E1 component		PRO_0000288750
Regions
	Domain	77 – 125	49	RPE1 insert

Sequences

	Sequence		Length	Mass (Da)
	Q4UKI8-1 [UniParc]. Last modified May 29, 2007. Version 2. Checksum: B0B3FAD38BA0533F	FASTA	977	109,496
	10 20 30 40 50 60 MEEDLKKTGY LFGGNAVFVD ELYRQYLANP ASVDQTWQEF FAGIKDNSTV LNKSTAKIII 70 80 90 100 110 120 PYEIKKEPLN NNLSSEVLNN RHLAKPAYRE EFKGDTERST AAYIDIREDA STGSTSKLPL 130 140 150 160 170 180 EAKFGKMSSL KAKEMINTYR KHAHYLANLD PLGLELRKTK NDLKLNIETF GLDNSQLEKN 190 200 210 220 230 240 INITDEFVGN WNCKLSELVT KLDKTYTGSI GVEFEQIENV EEKNWLYNKL ESEVTFSSED 250 260 270 280 290 300 KKTILNDLVE VEGFEQYLHT KFPGAKRFSV EGGDASIVAM SKAIDLSMNQ GVEEIVIGMA 310 320 330 340 350 360 HRGRLNTLTK VVGKPYKAVI AGFISGSVFP DELNVSGDVK YHLGYSSDRT IDNKKIHLSL 370 380 390 400 410 420 ADNPSHLEAV NPIVAGKVRA KQDILGDTKR SKVKAILVHG DAAFCGQGVV AESLSMSPLA 430 440 450 460 470 480 AYDIGGILHF VINNQLGFTA NAADTRASRY STEFAKIIAA PILHVNGDDI EAVLKATNIA 490 500 510 520 530 540 VEYRQKFGKD VVVEIICYRK YGHNEGDEPM YTQGKMYNII KSKPTPGNIY ANELVKSGII 550 560 570 580 590 600 DNNYFAKLKE EFKAKLDKEF EQAKNYKPEA HFLGGLWQGI SRIRTQAAIT GVGKKTLQDL 610 620 630 640 650 660 GTKLCEIPKD FAVNPKLVKL FEARKATLTS DQPIDWATAE QLAFASLLSE GTNIRLTGQD 670 680 690 700 710 720 CGRGTFSHRH SVLHNQIDDT TYIPLNNLSK TQAKYEVADS NLSEYAVLGF EYGYSLANPK 730 740 750 760 770 780 NLVLWEAQFG DFANGAQIIF DQFISSSETK WLRMSGLVVL LPHAFEGQGP EHSSARLERF 790 800 810 820 830 840 LQLAAEDNMY VTYPTTPASI FHLLRRQILD DTRKPLIIMS PKSLLRHKYA VSKLDELGEN 850 860 870 880 890 900 TTFLPVLDEV NKVDTNNITK VILCSGKVYY DLFEMRGNNS NIAIIRLEQL YPFEKKLVAS 910 920 930 940 950 960 LLKKYNRTQE FIWCQEEPKN MGAWRYIVSH LNDVLKEAGI NNEFKYVGRE ESASPAVGSL 970 QAHNKQQEKL LKEALGM

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000053 Genomic DNA. Translation: AAY61943.1. Different initiation.
RefSeq	YP_247108.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3400202.
GenomeReviews	Gene locus RF_1092 in contig CP000053_GR.
KEGG	rfe:RF_1092.
NMPDR	fig|315456.3.peg.1091.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4UKI8.
OMA	Q4UKI8. EGDEPAF.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_1092-MON.
BRENDA	1.2.4.2. 297363.
Family and domain databases
InterPro	IPR011603. 2oxoglutarate_DH_E1. IPR001017. DH_E1. IPR005728. Rickett_RPE. IPR005475. Transketolase_central-reg. [Graphical view]
PANTHER	PTHR23152. 2oxoglutarate_DH_E1. 1 hit.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. [Graphical view]
PIRSF	PIRSF000157. Oxoglu_dh_E1. 1 hit.
TIGRFAMs	TIGR00239. 2oxo_dh_E1. 1 hit. TIGR01045. RPE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ODO1_RICFE

Accession

Primary (citable) accession number: Q4UKI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 29, 2007
Last modified:	June 16, 2009
This is version 27 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)