Q4UKI6 (QUED_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-carboxy-5,6,7,8-tetrahydropterin synthase
Short name=CPH4 synthase
EC=4.-.-.-

Alternative name(s):
Queuosine biosynthesis protein queD

Gene names

Name:	queD
Ordered Locus Names:	RF_1094

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde By similarity.
Catalytic activity	7,8-dihydroneopterin 3'-triphosphate + H2O = 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + triphosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.
Miscellaneous	The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit By similarity.
Sequence similarities	Belongs to the PTPS family. QueD subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tetrahydrobiopterin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	6-pyruvoyltetrahydropterin synthase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 138	138	6-carboxy-5,6,7,8-tetrahydropterin synthase		PRO_0000272631
Sites
	Active site	23	1	Proton acceptor By similarity
	Active site	68	1	Charge relay system By similarity
	Active site	130	1	Charge relay system By similarity
	Metal binding	14	1	Zinc By similarity
	Metal binding	27	1	Zinc By similarity
	Metal binding	29	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UKI6 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: BEBBC34B5943EE46	FASTA	138	16,040
	10 20 30 40 50 60 MIKCTRRIEF DAGHRIIGHQ NKCQFLHGHR YVLEIAIAAN KTDKLGMVID FGLIKDVAKK 70 80 90 100 110 120 WIDENFDHSL ILHQDDKEMG QQIENCTGQK IYYMQNNPTA ENIATHLKNE IFPKLFVGQN 130 FFVSNLKLYE TPNCFVEV

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY61945.1.
RefSeq	YP_247110.1. NC_007109.1.
3D structure databases
ProteinModelPortal	Q4UKI6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3400204.
GenomeReviews	Gene locus RF_1094 in contig CP000053_GR.
KEGG	rfe:RF_1094.
NMPDR	fig|315456.3.peg.1093.
PATRIC	17893004. VBIRicFel64634_1317.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG727155.
OMA	GHKTVVL.
ProtClustDB	CLSK870781.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_1094-MONOMER.
Family and domain databases
InterPro	IPR007115. 6-PTP_synth-rel. [Graphical view]
KO	K01737.
Pfam	PF01242. PTPS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

QUED_RICFE

Accession

Primary (citable) accession number: Q4UKI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	July 5, 2005
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program