Reviewed, UniProtKB/Swiss-Prot Q4UKI6 (PTPS_RICFE)

Names and origin

Protein names

Recommended name:
    Putative 6-pyruvoyl tetrahydrobiopterin synthase
      Short name=PTP synthase
      Short name=PTPS
    EC=4.2.3.12

Gene names

Ordered Locus Names:

RF_1094

Organism

Rickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

42862 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	7,8-dihydroneopterin 3'-triphosphate = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Cofactor biosynthesis; tetrahydrobiopterin biosynthesis; tetrahydrobiopterin from 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate: step 1/3.
Subunit structure	Homohexamer formed of two homotrimers in a head to head fashion By similarity.
Miscellaneous	The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit.
Sequence similarities	Belongs to the PTPS family.

Ontologies

Keywords
Biological process	Tetrahydrobiopterin biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tetrahydrobiopterin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	6-pyruvoyltetrahydropterin synthase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 138	138	Putative 6-pyruvoyl tetrahydrobiopterin synthase		PRO_0000272631
Sites
	Active site	23	1	Proton acceptor By similarity
	Active site	68	1	Charge relay system By similarity
	Active site	130	1	Charge relay system By similarity
	Metal binding	14	1	Zinc By similarity
	Metal binding	27	1	Zinc By similarity
	Metal binding	29	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UKI6-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: BEBBC34B5943EE46	FASTA	138	16,040
	10 20 30 40 50 60 MIKCTRRIEF DAGHRIIGHQ NKCQFLHGHR YVLEIAIAAN KTDKLGMVID FGLIKDVAKK 70 80 90 100 110 120 WIDENFDHSL ILHQDDKEMG QQIENCTGQK IYYMQNNPTA ENIATHLKNE IFPKLFVGQN 130 FFVSNLKLYE TPNCFVEV

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000053 Genomic DNA. Translation: AAY61945.1.
RefSeq	YP_247110.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3400204.
GenomeReviews	Gene locus RF_1094 in contig CP000053_GR.
KEGG	rfe:RF_1094.
NMPDR	fig|315456.3.peg.1093.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4UKI6.
OMA	Q4UKI6. GHKTVVL.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_1094-MON.
BRENDA	4.2.3.12. 297363.
Family and domain databases
InterPro	IPR007115. 6-PTP_synth-rel. [Graphical view]
PANTHER	PTHR12589. 6_PTP_synth. 1 hit.
Pfam	PF01242. PTPS. 1 hit. [Graphical view]
ProDom	PD004049. PTPS_hypoth. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

PTPS_RICFE

Accession

Primary (citable) accession number: Q4UKI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	July 5, 2005
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)