Q4UKH4 (SRP54_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal recognition particle protein

Alternative name(s):
Fifty-four homolog

Gene names

Name:	ffh
Ordered Locus Names:	RF_1106

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components By similarity. HAMAP-Rule MF_00306
Subunit structure	Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. SRP is a ribonucleoprotein composed of Ffh and a 4.5S RNA molecule By similarity.
Subcellular location	Cytoplasm By similarity. Note: The SRP-RNC complex is targeted to the cytoplasmic membrane By similarity.
Domain	Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC By similarity. HAMAP-Rule MF_00306
Sequence similarities	Belongs to the GTP-binding SRP family. SRP54 subfamily.
Sequence caution	The sequence AAY61957.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Signal recognition particle
Ligand	GTP-binding Nucleotide-binding RNA-binding
Molecular function	Ribonucleoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC SRP-dependent cotranslational protein targeting to membrane Inferred from electronic annotation. Source: InterPro
Cellular_component	signal recognition particle, endoplasmic reticulum targeting Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	7S RNA binding Inferred from electronic annotation. Source: InterPro GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Signal recognition particle protein HAMAP-Rule MF_00306		PRO_0000286499
Regions
	Nucleotide binding	109 – 116	8	GTP By similarity
	Nucleotide binding	191 – 195	5	GTP By similarity
	Nucleotide binding	249 – 252	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UKH4 [UniParc]. Last modified May 15, 2007. Version 2. Checksum: EA73174669C97477	FASTA	449	49,318
	10 20 30 40 50 60 MFKTLTQNLT KIFDKLVSSG ILTEAQIDAA MRDIRVALLE SDVALPVIKD FIAEVKQKAL 70 80 90 100 110 120 GQEVIKSVSP GQMIIKIIHE EMINLLASSE SETKLNLNSK PPVNFLMVGL QGSGKTTASS 130 140 150 160 170 180 KLALRLKNQN KKVLLVSLDT YRPAAQEQLA ILANSVQINS LPIVQGEKPL DIVKRAIAEA 190 200 210 220 230 240 KISAYGVVIY DTAGRTQIDK EMMEEALAIK KIVEPTETLL VIDSMTGQDA VVTANSFNEK 250 260 270 280 290 300 LEISGLILSR IDGDSKGGAA LSVKYITKKP IKFLSSGEKL TDLEEFDAER LASRILDMGD 310 320 330 340 350 360 IISFVEKAAS IVDREEAEKT AAKLKKGKFD LNDYLQQMRS IKKMGGFGSI LSMLPGSGKI 370 380 390 400 410 420 MDQIDQSKLN SKIIEHQEAI ILSMTLKERE NPDIINASRR KRIAAGAGTT VQKVNILLKQ 430 440 YKQISEMMKK ASKMNPKNLL RNGIGKLFS

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY61957.1. Different initiation.
RefSeq	YP_247122.1. NC_007109.1.
3D structure databases
HSSP	HSSP built from PDB template 1LS1 based on UniProtKB O07347.
ProteinModelPortal	Q4UKH4.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_1106.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY61957; AAY61957; RF_1106.
GeneID	3400216.
KEGG	rfe:RF_1106.
PATRIC	17893034. VBIRicFel64634_1332.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0541.
HOGENOM	HOG000036164.
KO	K03106.
OMA	GQQVVKI.
ProtClustDB	PRK10867.
Family and domain databases
Gene3D	1.10.260.30. 1 hit.
HAMAP	MF_00306. SRP54.
InterPro	IPR003593. AAA+_ATPase. IPR013822. Signal_recog_particl_SRP54_hlx. IPR004125. Signal_recog_particle_SRP54_M. IPR022941. SRP54. IPR000897. SRP54_GTPase_dom. IPR004780. SRP_Ffh. [Graphical view]
PANTHER	PTHR11564:SF7. PTHR11564:SF7. 1 hit.
Pfam	PF00448. SRP54. 1 hit. PF02881. SRP54_N. 1 hit. PF02978. SRP_SPB. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00962. SRP54. 1 hit. SM00963. SRP54_N. 1 hit. [Graphical view]
SUPFAM	SSF47446. Signal_recog_particle_SRP54_M. 1 hit. SSF47364. SRP54. 1 hit.
TIGRFAMs	TIGR00959. ffh. 1 hit.
PROSITE	PS00300. SRP54. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SRP54_RICFE

Accession

Primary (citable) accession number: Q4UKH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	May 15, 2007
Last modified:	May 1, 2013
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program