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Q4UKH4 (SRP54_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Signal recognition particle protein
Alternative name(s):
Fifty-four homolog
Gene names
Name:ffh
Ordered Locus Names:RF_1106
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length449 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components By similarity. HAMAP-Rule MF_00306

Subunit structure

Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. SRP is a ribonucleoprotein composed of Ffh and a 4.5S RNA molecule By similarity.

Subcellular location

Cytoplasm By similarity. Note: The SRP-RNC complex is targeted to the cytoplasmic membrane By similarity. HAMAP-Rule MF_00306

Domain

Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC By similarity. HAMAP-Rule MF_00306

Sequence similarities

Belongs to the GTP-binding SRP family. SRP54 subfamily.

Sequence caution

The sequence AAY61957.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 449449Signal recognition particle protein HAMAP-Rule MF_00306
PRO_0000286499

Regions

Nucleotide binding109 – 1168GTP By similarity
Nucleotide binding191 – 1955GTP By similarity
Nucleotide binding249 – 2524GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4UKH4 [UniParc].

Last modified May 15, 2007. Version 2.
Checksum: EA73174669C97477

FASTA44949,318
        10         20         30         40         50         60 
MFKTLTQNLT KIFDKLVSSG ILTEAQIDAA MRDIRVALLE SDVALPVIKD FIAEVKQKAL 

        70         80         90        100        110        120 
GQEVIKSVSP GQMIIKIIHE EMINLLASSE SETKLNLNSK PPVNFLMVGL QGSGKTTASS 

       130        140        150        160        170        180 
KLALRLKNQN KKVLLVSLDT YRPAAQEQLA ILANSVQINS LPIVQGEKPL DIVKRAIAEA 

       190        200        210        220        230        240 
KISAYGVVIY DTAGRTQIDK EMMEEALAIK KIVEPTETLL VIDSMTGQDA VVTANSFNEK 

       250        260        270        280        290        300 
LEISGLILSR IDGDSKGGAA LSVKYITKKP IKFLSSGEKL TDLEEFDAER LASRILDMGD 

       310        320        330        340        350        360 
IISFVEKAAS IVDREEAEKT AAKLKKGKFD LNDYLQQMRS IKKMGGFGSI LSMLPGSGKI 

       370        380        390        400        410        420 
MDQIDQSKLN SKIIEHQEAI ILSMTLKERE NPDIINASRR KRIAAGAGTT VQKVNILLKQ 

       430        440 
YKQISEMMKK ASKMNPKNLL RNGIGKLFS 

« Hide

References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000053 Genomic DNA. Translation: AAY61957.1. Different initiation.
RefSeqYP_247122.1. NC_007109.1.

3D structure databases

ProteinModelPortalQ4UKH4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING315456.RF_1106.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY61957; AAY61957; RF_1106.
GeneID3400216.
KEGGrfe:RF_1106.
PATRIC17893034. VBIRicFel64634_1332.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0541.
HOGENOMHOG000036164.
KOK03106.
OMALKQHKQM.
OrthoDBEOG62K1ZH.

Enzyme and pathway databases

BioCycRETL1328306-WGS:GSTH-4045-MONOMER.
RFEL315456:GKEK-1138-MONOMER.

Family and domain databases

Gene3D1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00306. SRP54.
InterProIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR000897. SRP54_GTPase_dom.
IPR004780. SRP_Ffh.
[Graphical view]
PANTHERPTHR11564:SF7. PTHR11564:SF7. 1 hit.
PfamPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMSSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00959. ffh. 1 hit.
PROSITEPS00300. SRP54. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSRP54_RICFE
AccessionPrimary (citable) accession number: Q4UKH4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: July 9, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names