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Q4UKH4

- SRP54_RICFE

UniProt

Q4UKH4 - SRP54_RICFE

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Protein

Signal recognition particle protein

Gene

ffh

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi109 – 1168GTPUniRule annotation
Nucleotide bindingi191 – 1955GTPUniRule annotation
Nucleotide bindingi249 – 2524GTPUniRule annotation

GO - Molecular functioni

  1. 7S RNA binding Source: InterPro
  2. GTPase activity Source: UniProtKB-HAMAP
  3. GTP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. SRP-dependent cotranslational protein targeting to membrane Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-4045-MONOMER.
RFEL315456:GKEK-1138-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle proteinUniRule annotation
Alternative name(s):
Fifty-four homologUniRule annotation
Gene namesi
Name:ffhUniRule annotation
Ordered Locus Names:RF_1106
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000008548: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation
Note: The SRP-RNC complex is targeted to the cytoplasmic membrane.UniRule annotation

GO - Cellular componenti

  1. signal recognition particle, endoplasmic reticulum targeting Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Signal recognition particle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 449449Signal recognition particle proteinPRO_0000286499Add
BLAST

Interactioni

Subunit structurei

Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. SRP is a ribonucleoprotein composed of Ffh and a 4.5S RNA molecule.UniRule annotation

Protein-protein interaction databases

STRINGi315456.RF_1106.

Structurei

3D structure databases

ProteinModelPortaliQ4UKH4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.UniRule annotation

Sequence similaritiesi

Belongs to the GTP-binding SRP family. SRP54 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0541.
HOGENOMiHOG000036164.
KOiK03106.
OMAiLKQHKQM.
OrthoDBiEOG62K1ZH.

Family and domain databases

Gene3Di1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00306. SRP54.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR000897. SRP54_GTPase_dom.
IPR004780. SRP_Ffh.
[Graphical view]
PANTHERiPTHR11564:SF7. PTHR11564:SF7. 1 hit.
PfamiPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00959. ffh. 1 hit.
PROSITEiPS00300. SRP54. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4UKH4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFKTLTQNLT KIFDKLVSSG ILTEAQIDAA MRDIRVALLE SDVALPVIKD
60 70 80 90 100
FIAEVKQKAL GQEVIKSVSP GQMIIKIIHE EMINLLASSE SETKLNLNSK
110 120 130 140 150
PPVNFLMVGL QGSGKTTASS KLALRLKNQN KKVLLVSLDT YRPAAQEQLA
160 170 180 190 200
ILANSVQINS LPIVQGEKPL DIVKRAIAEA KISAYGVVIY DTAGRTQIDK
210 220 230 240 250
EMMEEALAIK KIVEPTETLL VIDSMTGQDA VVTANSFNEK LEISGLILSR
260 270 280 290 300
IDGDSKGGAA LSVKYITKKP IKFLSSGEKL TDLEEFDAER LASRILDMGD
310 320 330 340 350
IISFVEKAAS IVDREEAEKT AAKLKKGKFD LNDYLQQMRS IKKMGGFGSI
360 370 380 390 400
LSMLPGSGKI MDQIDQSKLN SKIIEHQEAI ILSMTLKERE NPDIINASRR
410 420 430 440
KRIAAGAGTT VQKVNILLKQ YKQISEMMKK ASKMNPKNLL RNGIGKLFS
Length:449
Mass (Da):49,318
Last modified:May 15, 2007 - v2
Checksum:iEA73174669C97477
GO

Sequence cautioni

The sequence AAY61957.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61957.1. Different initiation.
RefSeqiYP_247122.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY61957; AAY61957; RF_1106.
GeneIDi3400216.
KEGGirfe:RF_1106.
PATRICi17893034. VBIRicFel64634_1332.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61957.1 . Different initiation.
RefSeqi YP_247122.1. NC_007109.1.

3D structure databases

ProteinModelPortali Q4UKH4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 315456.RF_1106.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY61957 ; AAY61957 ; RF_1106 .
GeneIDi 3400216.
KEGGi rfe:RF_1106.
PATRICi 17893034. VBIRicFel64634_1332.

Phylogenomic databases

eggNOGi COG0541.
HOGENOMi HOG000036164.
KOi K03106.
OMAi LKQHKQM.
OrthoDBi EOG62K1ZH.

Enzyme and pathway databases

BioCyci RETL1328306-WGS:GSTH-4045-MONOMER.
RFEL315456:GKEK-1138-MONOMER.

Family and domain databases

Gene3Di 1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPi MF_00306. SRP54.
InterProi IPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR000897. SRP54_GTPase_dom.
IPR004780. SRP_Ffh.
[Graphical view ]
PANTHERi PTHR11564:SF7. PTHR11564:SF7. 1 hit.
Pfami PF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view ]
SUPFAMi SSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00959. ffh. 1 hit.
PROSITEi PS00300. SRP54. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.

Entry informationi

Entry nameiSRP54_RICFE
AccessioniPrimary (citable) accession number: Q4UKH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: October 1, 2014
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3