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Q4UKH4

- SRP54_RICFE

UniProt

Q4UKH4 - SRP54_RICFE

Protein

Signal recognition particle protein

Gene

ffh

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 2 (15 May 2007)
      Previous versions | rss
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    Functioni

    Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components.UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi109 – 1168GTPUniRule annotation
    Nucleotide bindingi191 – 1955GTPUniRule annotation
    Nucleotide bindingi249 – 2524GTPUniRule annotation

    GO - Molecular functioni

    1. 7S RNA binding Source: InterPro
    2. GTPase activity Source: UniProtKB-HAMAP
    3. GTP binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. SRP-dependent cotranslational protein targeting to membrane Source: InterPro

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciRETL1328306-WGS:GSTH-4045-MONOMER.
    RFEL315456:GKEK-1138-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Signal recognition particle proteinUniRule annotation
    Alternative name(s):
    Fifty-four homologUniRule annotation
    Gene namesi
    Name:ffhUniRule annotation
    Ordered Locus Names:RF_1106
    OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
    Taxonomic identifieri315456 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
    ProteomesiUP000008548: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation
    Note: The SRP-RNC complex is targeted to the cytoplasmic membrane.UniRule annotation

    GO - Cellular componenti

    1. signal recognition particle, endoplasmic reticulum targeting Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Signal recognition particle

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 449449Signal recognition particle proteinPRO_0000286499Add
    BLAST

    Interactioni

    Subunit structurei

    Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. SRP is a ribonucleoprotein composed of Ffh and a 4.5S RNA molecule.UniRule annotation

    Protein-protein interaction databases

    STRINGi315456.RF_1106.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4UKH4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.UniRule annotation

    Sequence similaritiesi

    Belongs to the GTP-binding SRP family. SRP54 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0541.
    HOGENOMiHOG000036164.
    KOiK03106.
    OMAiLKQHKQM.
    OrthoDBiEOG62K1ZH.

    Family and domain databases

    Gene3Di1.10.260.30. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPiMF_00306. SRP54.
    InterProiIPR003593. AAA+_ATPase.
    IPR027417. P-loop_NTPase.
    IPR013822. Signal_recog_particl_SRP54_hlx.
    IPR004125. Signal_recog_particle_SRP54_M.
    IPR022941. SRP54.
    IPR000897. SRP54_GTPase_dom.
    IPR004780. SRP_Ffh.
    [Graphical view]
    PANTHERiPTHR11564:SF7. PTHR11564:SF7. 1 hit.
    PfamiPF00448. SRP54. 1 hit.
    PF02881. SRP54_N. 1 hit.
    PF02978. SRP_SPB. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    SM00962. SRP54. 1 hit.
    SM00963. SRP54_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF47446. SSF47446. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00959. ffh. 1 hit.
    PROSITEiPS00300. SRP54. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4UKH4-1 [UniParc]FASTAAdd to Basket

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    MFKTLTQNLT KIFDKLVSSG ILTEAQIDAA MRDIRVALLE SDVALPVIKD    50
    FIAEVKQKAL GQEVIKSVSP GQMIIKIIHE EMINLLASSE SETKLNLNSK 100
    PPVNFLMVGL QGSGKTTASS KLALRLKNQN KKVLLVSLDT YRPAAQEQLA 150
    ILANSVQINS LPIVQGEKPL DIVKRAIAEA KISAYGVVIY DTAGRTQIDK 200
    EMMEEALAIK KIVEPTETLL VIDSMTGQDA VVTANSFNEK LEISGLILSR 250
    IDGDSKGGAA LSVKYITKKP IKFLSSGEKL TDLEEFDAER LASRILDMGD 300
    IISFVEKAAS IVDREEAEKT AAKLKKGKFD LNDYLQQMRS IKKMGGFGSI 350
    LSMLPGSGKI MDQIDQSKLN SKIIEHQEAI ILSMTLKERE NPDIINASRR 400
    KRIAAGAGTT VQKVNILLKQ YKQISEMMKK ASKMNPKNLL RNGIGKLFS 449
    Length:449
    Mass (Da):49,318
    Last modified:May 15, 2007 - v2
    Checksum:iEA73174669C97477
    GO

    Sequence cautioni

    The sequence AAY61957.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000053 Genomic DNA. Translation: AAY61957.1. Different initiation.
    RefSeqiYP_247122.1. NC_007109.1.

    Genome annotation databases

    EnsemblBacteriaiAAY61957; AAY61957; RF_1106.
    GeneIDi3400216.
    KEGGirfe:RF_1106.
    PATRICi17893034. VBIRicFel64634_1332.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000053 Genomic DNA. Translation: AAY61957.1 . Different initiation.
    RefSeqi YP_247122.1. NC_007109.1.

    3D structure databases

    ProteinModelPortali Q4UKH4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 315456.RF_1106.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY61957 ; AAY61957 ; RF_1106 .
    GeneIDi 3400216.
    KEGGi rfe:RF_1106.
    PATRICi 17893034. VBIRicFel64634_1332.

    Phylogenomic databases

    eggNOGi COG0541.
    HOGENOMi HOG000036164.
    KOi K03106.
    OMAi LKQHKQM.
    OrthoDBi EOG62K1ZH.

    Enzyme and pathway databases

    BioCyci RETL1328306-WGS:GSTH-4045-MONOMER.
    RFEL315456:GKEK-1138-MONOMER.

    Family and domain databases

    Gene3Di 1.10.260.30. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPi MF_00306. SRP54.
    InterProi IPR003593. AAA+_ATPase.
    IPR027417. P-loop_NTPase.
    IPR013822. Signal_recog_particl_SRP54_hlx.
    IPR004125. Signal_recog_particle_SRP54_M.
    IPR022941. SRP54.
    IPR000897. SRP54_GTPase_dom.
    IPR004780. SRP_Ffh.
    [Graphical view ]
    PANTHERi PTHR11564:SF7. PTHR11564:SF7. 1 hit.
    Pfami PF00448. SRP54. 1 hit.
    PF02881. SRP54_N. 1 hit.
    PF02978. SRP_SPB. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    SM00962. SRP54. 1 hit.
    SM00963. SRP54_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47446. SSF47446. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00959. ffh. 1 hit.
    PROSITEi PS00300. SRP54. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
      Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
      PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC VR-1525 / URRWXCal2.

    Entry informationi

    Entry nameiSRP54_RICFE
    AccessioniPrimary (citable) accession number: Q4UKH4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 15, 2007
    Last sequence update: May 15, 2007
    Last modified: October 1, 2014
    This is version 71 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Rickettsia felis (Rickettsia azadi)
      Rickettsia felis (strain URRWXCal2): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3