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Protein

Ribosomal RNA large subunit methyltransferase E

Gene

rlmE

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(2552) in 23S rRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei78S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei80S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei103S-adenosyl-L-methionineUniRule annotation1
Binding sitei119S-adenosyl-L-methionineUniRule annotation1
Binding sitei143S-adenosyl-L-methionineUniRule annotation1
Active sitei183Proton acceptorUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA large subunit methyltransferase EUniRule annotation (EC:2.1.1.166UniRule annotation)
Alternative name(s):
23S rRNA Um2552 methyltransferaseUniRule annotation
rRNA (uridine-2'-O-)-methyltransferaseUniRule annotation
Gene namesi
Name:rlmEUniRule annotation
Synonyms:ftsJUniRule annotation, rrmJUniRule annotation
Ordered Locus Names:RF_1118
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
Proteomesi
  • UP000008548 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001555331 – 227Ribosomal RNA large subunit methyltransferase EAdd BLAST227

Interactioni

Protein-protein interaction databases

STRINGi315456.RF_1118.

Structurei

3D structure databases

ProteinModelPortaliQ4UKG2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F7M. Bacteria.
COG0293. LUCA.
HOGENOMiHOG000162367.
KOiK02427.
OMAiIDIPRAM.
OrthoDBiPOG091H05ST.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01547. RNA_methyltr_E. 1 hit.
InterProiIPR015507. rRNA-MeTfrase_E.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10920. PTHR10920. 1 hit.
PfamiPF01728. FtsJ. 1 hit.
[Graphical view]
PIRSFiPIRSF005461. 23S_rRNA_mtase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4UKG2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNNLSGYRN KFVRVKTSKK RTVSSSNWLR RQLNDPYVAK ARLEGFRSRA
60 70 80 90 100
AYKLLEIHGK FKLFNPNMKI VDLGAAPGGW SQVASKLIKA SDNSLNNKII
110 120 130 140 150
SIDLLEIEPI AEVEFFQKDF FEENTEELII QALDGKADIV MSDMASNTIG
160 170 180 190 200
HKATDHIRTL LLCEQAFEFA LKVLKPSGHF IAKIFRGGAE NELLNKVKRE
210 220
FRTVKHFKPS SSRSESTEIY LVALNKK
Length:227
Mass (Da):25,764
Last modified:July 5, 2005 - v1
Checksum:iA7528B1C4DC19D56
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61969.1.
RefSeqiWP_011271428.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY61969; AAY61969; RF_1118.
KEGGirfe:RF_1118.
PATRICi17893068. VBIRicFel64634_1349.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61969.1.
RefSeqiWP_011271428.1. NC_007109.1.

3D structure databases

ProteinModelPortaliQ4UKG2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi315456.RF_1118.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY61969; AAY61969; RF_1118.
KEGGirfe:RF_1118.
PATRICi17893068. VBIRicFel64634_1349.

Phylogenomic databases

eggNOGiENOG4105F7M. Bacteria.
COG0293. LUCA.
HOGENOMiHOG000162367.
KOiK02427.
OMAiIDIPRAM.
OrthoDBiPOG091H05ST.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01547. RNA_methyltr_E. 1 hit.
InterProiIPR015507. rRNA-MeTfrase_E.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10920. PTHR10920. 1 hit.
PfamiPF01728. FtsJ. 1 hit.
[Graphical view]
PIRSFiPIRSF005461. 23S_rRNA_mtase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRLME_RICFE
AccessioniPrimary (citable) accession number: Q4UKG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: July 5, 2005
Last modified: November 2, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.