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Q4UKG2

- RLME_RICFE

UniProt

Q4UKG2 - RLME_RICFE

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Protein

Ribosomal RNA large subunit methyltransferase E

Gene

rlmE

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(2552) in 23S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei78 – 781S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei80 – 801S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei103 – 1031S-adenosyl-L-methionineUniRule annotation
Binding sitei119 – 1191S-adenosyl-L-methionineUniRule annotation
Binding sitei143 – 1431S-adenosyl-L-methionineUniRule annotation
Active sitei183 – 1831Proton acceptorUniRule annotation

GO - Molecular functioni

  1. rRNA (uridine-2'-O-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-1150-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA large subunit methyltransferase EUniRule annotation (EC:2.1.1.166UniRule annotation)
Alternative name(s):
23S rRNA Um2552 methyltransferaseUniRule annotation
rRNA (uridine-2'-O-)-methyltransferaseUniRule annotation
Gene namesi
Name:rlmEUniRule annotation
Synonyms:ftsJUniRule annotation, rrmJUniRule annotation
Ordered Locus Names:RF_1118
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000008548: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227Ribosomal RNA large subunit methyltransferase EPRO_0000155533Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi315456.RF_1118.

Structurei

3D structure databases

ProteinModelPortaliQ4UKG2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0293.
HOGENOMiHOG000162367.
KOiK02427.
OMAiQYVVSQI.
OrthoDBiEOG657JC0.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01547. RNA_methyltr_E.
InterProiIPR015507. rRNA-MeTfrase_E.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10920. PTHR10920. 1 hit.
PfamiPF01728. FtsJ. 1 hit.
[Graphical view]
PIRSFiPIRSF005461. 23S_rRNA_mtase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4UKG2 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTNNLSGYRN KFVRVKTSKK RTVSSSNWLR RQLNDPYVAK ARLEGFRSRA
60 70 80 90 100
AYKLLEIHGK FKLFNPNMKI VDLGAAPGGW SQVASKLIKA SDNSLNNKII
110 120 130 140 150
SIDLLEIEPI AEVEFFQKDF FEENTEELII QALDGKADIV MSDMASNTIG
160 170 180 190 200
HKATDHIRTL LLCEQAFEFA LKVLKPSGHF IAKIFRGGAE NELLNKVKRE
210 220
FRTVKHFKPS SSRSESTEIY LVALNKK
Length:227
Mass (Da):25,764
Last modified:July 5, 2005 - v1
Checksum:iA7528B1C4DC19D56
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000053 Genomic DNA. Translation: AAY61969.1.
RefSeqiYP_247134.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY61969; AAY61969; RF_1118.
GeneIDi3401414.
KEGGirfe:RF_1118.
PATRICi17893068. VBIRicFel64634_1349.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000053 Genomic DNA. Translation: AAY61969.1 .
RefSeqi YP_247134.1. NC_007109.1.

3D structure databases

ProteinModelPortali Q4UKG2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 315456.RF_1118.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY61969 ; AAY61969 ; RF_1118 .
GeneIDi 3401414.
KEGGi rfe:RF_1118.
PATRICi 17893068. VBIRicFel64634_1349.

Phylogenomic databases

eggNOGi COG0293.
HOGENOMi HOG000162367.
KOi K02427.
OMAi QYVVSQI.
OrthoDBi EOG657JC0.

Enzyme and pathway databases

BioCyci RFEL315456:GKEK-1150-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01547. RNA_methyltr_E.
InterProi IPR015507. rRNA-MeTfrase_E.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10920. PTHR10920. 1 hit.
Pfami PF01728. FtsJ. 1 hit.
[Graphical view ]
PIRSFi PIRSF005461. 23S_rRNA_mtase. 1 hit.
SUPFAMi SSF53335. SSF53335. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.

Entry informationi

Entry nameiRLME_RICFE
AccessioniPrimary (citable) accession number: Q4UKG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: July 5, 2005
Last modified: October 29, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3