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Q4UKG2 (RLME_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosomal RNA large subunit methyltransferase E

EC=2.1.1.166
Alternative name(s):
23S rRNA Um2552 methyltransferase
rRNA (uridine-2'-O-)-methyltransferase
Gene names
Name:rlmE
Synonyms:ftsJ, rrmJ
Ordered Locus Names:RF_1118
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length227 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit By similarity. HAMAP-Rule MF_01547

Catalytic activity

S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(2552) in 23S rRNA. HAMAP-Rule MF_01547

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01547.

Sequence similarities

Belongs to the methyltransferase superfamily. RlmE family.

Ontologies

Keywords
   Biological processrRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionrRNA (uridine-2'-O-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 227227Ribosomal RNA large subunit methyltransferase E HAMAP-Rule MF_01547
PRO_0000155533

Sites

Active site1831Proton acceptor By similarity
Binding site781S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site801S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site1031S-adenosyl-L-methionine By similarity
Binding site1191S-adenosyl-L-methionine By similarity
Binding site1431S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4UKG2 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: A7528B1C4DC19D56

FASTA22725,764
        10         20         30         40         50         60 
MTNNLSGYRN KFVRVKTSKK RTVSSSNWLR RQLNDPYVAK ARLEGFRSRA AYKLLEIHGK 

        70         80         90        100        110        120 
FKLFNPNMKI VDLGAAPGGW SQVASKLIKA SDNSLNNKII SIDLLEIEPI AEVEFFQKDF 

       130        140        150        160        170        180 
FEENTEELII QALDGKADIV MSDMASNTIG HKATDHIRTL LLCEQAFEFA LKVLKPSGHF 

       190        200        210        220 
IAKIFRGGAE NELLNKVKRE FRTVKHFKPS SSRSESTEIY LVALNKK 

« Hide

References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000053 Genomic DNA. Translation: AAY61969.1.
RefSeqYP_247134.1. NC_007109.1.

3D structure databases

ProteinModelPortalQ4UKG2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING315456.RF_1118.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY61969; AAY61969; RF_1118.
GeneID3401414.
KEGGrfe:RF_1118.
PATRIC17893068. VBIRicFel64634_1349.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0293.
HOGENOMHOG000162367.
KOK02427.
OMAQYVVSQI.
OrthoDBEOG657JC0.

Enzyme and pathway databases

BioCycRFEL315456:GKEK-1150-MONOMER.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01547. RNA_methyltr_E.
InterProIPR015507. rRNA-MeTfrase_E.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10920. PTHR10920. 1 hit.
PfamPF01728. FtsJ. 1 hit.
[Graphical view]
PIRSFPIRSF005461. 23S_rRNA_mtase. 1 hit.
SUPFAMSSF53335. SSF53335. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRLME_RICFE
AccessionPrimary (citable) accession number: Q4UKG2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: July 5, 2005
Last modified: June 11, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names