Q4UKG2 (RLME_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase E
EC=2.1.1.166

Alternative name(s):
23S rRNA Um2552 methyltransferase
rRNA (uridine-2'-O-)-methyltransferase

Gene names

Name:	rlmE
Synonyms:	ftsJ, rrmJ
Ordered Locus Names:	RF_1118

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit By similarity. HAMAP-Rule MF_01547
Catalytic activity	S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(2552) in 23S rRNA. HAMAP-Rule MF_01547
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmE family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	rRNA (uridine-2'-O-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 227	227	Ribosomal RNA large subunit methyltransferase E HAMAP-Rule MF_01547		PRO_0000155533
Sites
	Active site	183	1	Proton acceptor By similarity
	Binding site	78	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	80	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	103	1	S-adenosyl-L-methionine By similarity
	Binding site	119	1	S-adenosyl-L-methionine By similarity
	Binding site	143	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UKG2 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: A7528B1C4DC19D56	FASTA	227	25,764
	10 20 30 40 50 60 MTNNLSGYRN KFVRVKTSKK RTVSSSNWLR RQLNDPYVAK ARLEGFRSRA AYKLLEIHGK 70 80 90 100 110 120 FKLFNPNMKI VDLGAAPGGW SQVASKLIKA SDNSLNNKII SIDLLEIEPI AEVEFFQKDF 130 140 150 160 170 180 FEENTEELII QALDGKADIV MSDMASNTIG HKATDHIRTL LLCEQAFEFA LKVLKPSGHF 190 200 210 220 IAKIFRGGAE NELLNKVKRE FRTVKHFKPS SSRSESTEIY LVALNKK

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY61969.1.
RefSeq	YP_247134.1. NC_007109.1.
3D structure databases
ProteinModelPortal	Q4UKG2.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_1118.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY61969; AAY61969; RF_1118.
GeneID	3401414.
KEGG	rfe:RF_1118.
PATRIC	17893068. VBIRicFel64634_1349.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0293.
HOGENOM	HOG000162367.
KO	K02427.
OMA	NDPYVQR.
ProtClustDB	CLSK870770.
Family and domain databases
HAMAP	MF_01547. RNA_methyltr_E.
InterPro	IPR015507. rRNA-MeTfrase_E. IPR002877. rRNA_MeTrfase_FtsJ_dom. [Graphical view]
PANTHER	PTHR10920. PTHR10920. 1 hit.
Pfam	PF01728. FtsJ. 1 hit. [Graphical view]
PIRSF	PIRSF005461. 23S_rRNA_mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLME_RICFE

Accession

Primary (citable) accession number: Q4UKG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	July 5, 2005
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program