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Q4UKG2

- RLME_RICFE

UniProt

Q4UKG2 - RLME_RICFE

Protein

Ribosomal RNA large subunit methyltransferase E

Gene

rlmE

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (05 Jul 2005)
      Previous versions | rss
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    • Comment

    Functioni

    Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(2552) in 23S rRNA.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei78 – 781S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
    Binding sitei80 – 801S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
    Binding sitei103 – 1031S-adenosyl-L-methionineUniRule annotation
    Binding sitei119 – 1191S-adenosyl-L-methionineUniRule annotation
    Binding sitei143 – 1431S-adenosyl-L-methionineUniRule annotation
    Active sitei183 – 1831Proton acceptorUniRule annotation

    GO - Molecular functioni

    1. rRNA (uridine-2'-O-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    rRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciRFEL315456:GKEK-1150-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribosomal RNA large subunit methyltransferase EUniRule annotation (EC:2.1.1.166UniRule annotation)
    Alternative name(s):
    23S rRNA Um2552 methyltransferaseUniRule annotation
    rRNA (uridine-2'-O-)-methyltransferaseUniRule annotation
    Gene namesi
    Name:rlmEUniRule annotation
    Synonyms:ftsJUniRule annotation, rrmJUniRule annotation
    Ordered Locus Names:RF_1118
    OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
    Taxonomic identifieri315456 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
    ProteomesiUP000008548: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 227227Ribosomal RNA large subunit methyltransferase EPRO_0000155533Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi315456.RF_1118.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4UKG2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the methyltransferase superfamily. RlmE family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0293.
    HOGENOMiHOG000162367.
    KOiK02427.
    OMAiQYVVSQI.
    OrthoDBiEOG657JC0.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01547. RNA_methyltr_E.
    InterProiIPR015507. rRNA-MeTfrase_E.
    IPR002877. rRNA_MeTrfase_FtsJ_dom.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10920. PTHR10920. 1 hit.
    PfamiPF01728. FtsJ. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005461. 23S_rRNA_mtase. 1 hit.
    SUPFAMiSSF53335. SSF53335. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q4UKG2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTNNLSGYRN KFVRVKTSKK RTVSSSNWLR RQLNDPYVAK ARLEGFRSRA    50
    AYKLLEIHGK FKLFNPNMKI VDLGAAPGGW SQVASKLIKA SDNSLNNKII 100
    SIDLLEIEPI AEVEFFQKDF FEENTEELII QALDGKADIV MSDMASNTIG 150
    HKATDHIRTL LLCEQAFEFA LKVLKPSGHF IAKIFRGGAE NELLNKVKRE 200
    FRTVKHFKPS SSRSESTEIY LVALNKK 227
    Length:227
    Mass (Da):25,764
    Last modified:July 5, 2005 - v1
    Checksum:iA7528B1C4DC19D56
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000053 Genomic DNA. Translation: AAY61969.1.
    RefSeqiWP_011271428.1. NC_007109.1.
    YP_247134.1. NC_007109.1.

    Genome annotation databases

    EnsemblBacteriaiAAY61969; AAY61969; RF_1118.
    GeneIDi3401414.
    KEGGirfe:RF_1118.
    PATRICi17893068. VBIRicFel64634_1349.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000053 Genomic DNA. Translation: AAY61969.1 .
    RefSeqi WP_011271428.1. NC_007109.1.
    YP_247134.1. NC_007109.1.

    3D structure databases

    ProteinModelPortali Q4UKG2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 315456.RF_1118.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY61969 ; AAY61969 ; RF_1118 .
    GeneIDi 3401414.
    KEGGi rfe:RF_1118.
    PATRICi 17893068. VBIRicFel64634_1349.

    Phylogenomic databases

    eggNOGi COG0293.
    HOGENOMi HOG000162367.
    KOi K02427.
    OMAi QYVVSQI.
    OrthoDBi EOG657JC0.

    Enzyme and pathway databases

    BioCyci RFEL315456:GKEK-1150-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01547. RNA_methyltr_E.
    InterProi IPR015507. rRNA-MeTfrase_E.
    IPR002877. rRNA_MeTrfase_FtsJ_dom.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10920. PTHR10920. 1 hit.
    Pfami PF01728. FtsJ. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005461. 23S_rRNA_mtase. 1 hit.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
      Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
      PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC VR-1525 / URRWXCal2.

    Entry informationi

    Entry nameiRLME_RICFE
    AccessioniPrimary (citable) accession number: Q4UKG2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: July 5, 2005
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Rickettsia felis (Rickettsia azadi)
      Rickettsia felis (strain URRWXCal2): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3