Q4UKF1 (GATA_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A
Short name=Glu-ADT subunit A
EC=6.3.5.-

Gene names

Name:	gatA
Ordered Locus Names:	RF_1129

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00120
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00120
Subunit structure	Heterotrimer of A, B and C subunits By similarity.
Sequence similarities	Belongs to the amidase family. GatA subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	Glutamyl-tRNA(Gln) amidotransferase subunit A HAMAP-Rule MF_00120		PRO_0000241149
Sites
	Active site	78	1	Charge relay system By similarity
	Active site	158	1	Charge relay system By similarity
	Active site	182	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UKF1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 1BA841879A33C182	FASTA	493	53,180
	10 20 30 40 50 60 MTELNKLTVA DSVKGLKNKD FTSKELVNAH IKQIEKHKNL NAYVTETFDL ALKQAEAADQ 70 80 90 100 110 120 NYAQNQPQTL EGIPFAAKDL FCTKGIRTTA CSNILKNFIP NYESSVTQNI FDKGGVMLGK 130 140 150 160 170 180 TNMDEFAMGS ANITSCFGNV ISPWKANDDN ADLVPGGSSG GSAAAVSGFM ASAALGSDTG 190 200 210 220 230 240 GSVRQPASFT GLVGFKPTYG RCSRYGMVSF ASSLDQAGIF TRSVLDSSIM LEAMMGFDEK 250 260 270 280 290 300 DSTSIKAEVP ELQSAIGSSM KNMKIGVPLS LGEGGIIEPD IMKMWQDTIE LLKNAGAEIV 310 320 330 340 350 360 DITLPHAKYG VAVYYVIAPA EASSNLSRYD GVRYGLRVER ENMTLDEMYE MTRSAGFGEE 370 380 390 400 410 420 VKRRIMIGTY VLSSSCMDAY YLKAQKVRRL VANDFNNAFA KVDAILLPAA PTEAFKIGEK 430 440 450 460 470 480 QNDPTIMYLN DLFTIPASLA GLPCASVPAG LSARGLPLGM QIIGKQLDEY NVLKVASTIE 490 SGVKHIKFEP KGF

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY61980.1.
RefSeq	YP_247145.1. NC_007109.1.
3D structure databases
ProteinModelPortal	Q4UKF1.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_1129.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY61980; AAY61980; RF_1129.
GeneID	3401426.
KEGG	rfe:RF_1129.
PATRIC	17893100. VBIRicFel64634_1364.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0154.
HOGENOM	HOG000116699.
KO	K02433.
OMA	RYDGVKY.
ProtClustDB	PRK00012.
Family and domain databases
Gene3D	3.90.1300.10. 1 hit.
HAMAP	MF_00120. GatA.
InterPro	IPR000120. Amidase. IPR020556. Amidase_CS. IPR023631. Amidase_dom. IPR004412. GatA. [Graphical view]
PANTHER	PTHR11895. PTHR11895. 1 hit.
Pfam	PF01425. Amidase. 1 hit. [Graphical view]
SUPFAM	SSF75304. Amidase_sig_enz. 1 hit.
TIGRFAMs	TIGR00132. gatA. 1 hit.
PROSITE	PS00571. AMIDASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATA_RICFE

Accession

Primary (citable) accession number: Q4UKF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	July 5, 2005
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program