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Q4UKF1 (GATA_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A

Short name=Glu-ADT subunit A
EC=6.3.5.7
Gene names
Name:gatA
Ordered Locus Names:RF_1129
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length493 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00120

Catalytic activity

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00120

Subunit structure

Heterotrimer of A, B and C subunits By similarity. HAMAP-Rule MF_00120

Sequence similarities

Belongs to the amidase family. GatA subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtranslation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 493493Glutamyl-tRNA(Gln) amidotransferase subunit A HAMAP-Rule MF_00120
PRO_0000241149

Sites

Active site781Charge relay system By similarity
Active site1581Charge relay system By similarity
Active site1821Acyl-ester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4UKF1 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: 1BA841879A33C182

FASTA49353,180
        10         20         30         40         50         60 
MTELNKLTVA DSVKGLKNKD FTSKELVNAH IKQIEKHKNL NAYVTETFDL ALKQAEAADQ 

        70         80         90        100        110        120 
NYAQNQPQTL EGIPFAAKDL FCTKGIRTTA CSNILKNFIP NYESSVTQNI FDKGGVMLGK 

       130        140        150        160        170        180 
TNMDEFAMGS ANITSCFGNV ISPWKANDDN ADLVPGGSSG GSAAAVSGFM ASAALGSDTG 

       190        200        210        220        230        240 
GSVRQPASFT GLVGFKPTYG RCSRYGMVSF ASSLDQAGIF TRSVLDSSIM LEAMMGFDEK 

       250        260        270        280        290        300 
DSTSIKAEVP ELQSAIGSSM KNMKIGVPLS LGEGGIIEPD IMKMWQDTIE LLKNAGAEIV 

       310        320        330        340        350        360 
DITLPHAKYG VAVYYVIAPA EASSNLSRYD GVRYGLRVER ENMTLDEMYE MTRSAGFGEE 

       370        380        390        400        410        420 
VKRRIMIGTY VLSSSCMDAY YLKAQKVRRL VANDFNNAFA KVDAILLPAA PTEAFKIGEK 

       430        440        450        460        470        480 
QNDPTIMYLN DLFTIPASLA GLPCASVPAG LSARGLPLGM QIIGKQLDEY NVLKVASTIE 

       490 
SGVKHIKFEP KGF 

« Hide

References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000053 Genomic DNA. Translation: AAY61980.1.
RefSeqYP_247145.1. NC_007109.1.

3D structure databases

ProteinModelPortalQ4UKF1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING315456.RF_1129.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY61980; AAY61980; RF_1129.
GeneID3401426.
KEGGrfe:RF_1129.
PATRIC17893100. VBIRicFel64634_1364.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0154.
HOGENOMHOG000116699.
KOK02433.
OMARDSTSID.
OrthoDBEOG61P6R9.
ProtClustDBPRK00012.

Enzyme and pathway databases

BioCycRFEL315456:GKEK-1162-MONOMER.

Family and domain databases

Gene3D3.90.1300.10. 1 hit.
HAMAPMF_00120. GatA.
InterProIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERPTHR11895. PTHR11895. 1 hit.
PfamPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMSSF75304. SSF75304. 1 hit.
TIGRFAMsTIGR00132. gatA. 1 hit.
PROSITEPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGATA_RICFE
AccessionPrimary (citable) accession number: Q4UKF1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: July 5, 2005
Last modified: March 19, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names