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Q4UKE2

- SYDND_RICFE

UniProt

Q4UKE2 - SYDND_RICFE

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Protein

Aspartate--tRNA(Asp/Asn) ligase

Gene
aspS, RF_1138
Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn) By similarity.UniRule annotation

Catalytic activityi

ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei33 – 331Important for tRNA non-discrimination By similarity
Binding sitei176 – 1761Aspartate By similarity
Binding sitei222 – 2221Aspartate By similarity
Binding sitei452 – 4521Aspartate By similarity
Binding sitei490 – 4901ATP By similarity
Binding sitei493 – 4931Aspartate By similarity
Binding sitei497 – 4971Aspartate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi222 – 2243ATP By similarity
Nucleotide bindingi542 – 5454ATP By similarity

GO - Molecular functioni

  1. aspartate-tRNA(Asn) ligase activity Source: UniProtKB-EC
  2. aspartate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. ATP binding Source: UniProtKB-HAMAP
  4. nucleic acid binding Source: InterPro

GO - Biological processi

  1. aspartyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-1171-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA(Asp/Asn) ligase (EC:6.1.1.23)
Alternative name(s):
Aspartyl-tRNA synthetase
Short name:
AspRS
Non-discriminating aspartyl-tRNA synthetase
Short name:
ND-AspRS
Gene namesi
Name:aspS
Ordered Locus Names:RF_1138
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000008548: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 600600Aspartate--tRNA(Asp/Asn) ligaseUniRule annotationPRO_0000235553Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi315456.RF_1138.

Structurei

3D structure databases

ProteinModelPortaliQ4UKE2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni200 – 2034Aspartate By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0173.
HOGENOMiHOG000275160.
KOiK01876.
OMAiSLSRKQI.
OrthoDBiEOG68Q0NX.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.1360.30. 1 hit.
HAMAPiMF_00044. Asp_tRNA_synth.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF5. PTHR22594:SF5. 1 hit.
PfamiPF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4UKE2-1 [UniParc]FASTAAdd to Basket

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MHKYRTHNCN ELQISDVRKE VKLSGWVHRR RDHGNLVFID LRDHYGITQI    50
VFTDQNQQLM EDASRLRYES VITVRGTIVA RSNDTINDTL PTGHIEVLAG 100
EFIVESAADT LPFVINTEKD APEETRLKHR FLDLRREKLH NNIILRSQII 150
SHIRHLMTGR GFTEFQTPIL TASSPEGARD FLVPSRMHPG KFYALPQAPQ 200
QFKQLLMVSG FDRYFQIAPC FRDEDARADR SPGEFYQLDV EMSFVTQEDI 250
FSTIEPVMYD LFTKFTAKKV SETPFVRIPY NESMLKYGSD KPDLRNPIII 300
ADVTEIFRDS DFTIFRENIK KGSIVRAIPA PKAAALPRSF FDKMIEFAIS 350
EGAGGLGYIQ FSETGEAKGP VAKFLSPQQL ESLKATASIS NGDAVFFASD 400
KKEKAAKLAG KVRIRLGEEL DLLEKDCFKF CWITDFPFYE LNEDTGKIDF 450
SHNPFSMPQG GLEALEQAKT TEELLELTAY QYDIVCNGIE LSSGAIRNHK 500
PEIMYKAFSM AGYSEEEVDK RFGGMIRAFK FGAPPHGGIA PGIDRIVMLL 550
AEATNIREII AFPLNQQAED LLMNAPSYVE DKALKELSIM LSPSILKNMK 600
Length:600
Mass (Da):67,976
Last modified:July 5, 2005 - v1
Checksum:i4FF6DFE4682E5264
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000053 Genomic DNA. Translation: AAY61989.1.
RefSeqiYP_247154.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY61989; AAY61989; RF_1138.
GeneIDi3401435.
KEGGirfe:RF_1138.
PATRICi17893120. VBIRicFel64634_1374.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000053 Genomic DNA. Translation: AAY61989.1 .
RefSeqi YP_247154.1. NC_007109.1.

3D structure databases

ProteinModelPortali Q4UKE2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 315456.RF_1138.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY61989 ; AAY61989 ; RF_1138 .
GeneIDi 3401435.
KEGGi rfe:RF_1138.
PATRICi 17893120. VBIRicFel64634_1374.

Phylogenomic databases

eggNOGi COG0173.
HOGENOMi HOG000275160.
KOi K01876.
OMAi SLSRKQI.
OrthoDBi EOG68Q0NX.

Enzyme and pathway databases

BioCyci RFEL315456:GKEK-1171-MONOMER.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
3.30.1360.30. 1 hit.
HAMAPi MF_00044. Asp_tRNA_synth.
InterProi IPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view ]
PANTHERi PTHR22594. PTHR22594. 1 hit.
PTHR22594:SF5. PTHR22594:SF5. 1 hit.
Pfami PF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view ]
PRINTSi PR01042. TRNASYNTHASP.
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsi TIGR00459. aspS_bact. 1 hit.
PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.

Entry informationi

Entry nameiSYDND_RICFE
AccessioniPrimary (citable) accession number: Q4UKE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: July 5, 2005
Last modified: July 9, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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