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Q4UKE2 (SYD_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aspartate--tRNA ligase
EC=6.1.1.12
Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS
Gene names
Name:aspS
Ordered Locus Names:RF_1138
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group ›

Protein attributes

Sequence length600 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP-Rule MF_00044

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processaspartyl-tRNA aminoacylation

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: HAMAP

aspartate-tRNA ligase activity

Inferred from electronic annotation. Source: HAMAP

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 600600Aspartate--tRNA ligase HAMAP-Rule MF_00044
PRO_0000235553

Sequences

Sequence LengthMass (Da)Tools
Q4UKE2 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: 4FF6DFE4682E5264

FASTA60067,976
        10         20         30         40         50         60 
MHKYRTHNCN ELQISDVRKE VKLSGWVHRR RDHGNLVFID LRDHYGITQI VFTDQNQQLM 

        70         80         90        100        110        120 
EDASRLRYES VITVRGTIVA RSNDTINDTL PTGHIEVLAG EFIVESAADT LPFVINTEKD 

       130        140        150        160        170        180 
APEETRLKHR FLDLRREKLH NNIILRSQII SHIRHLMTGR GFTEFQTPIL TASSPEGARD 

       190        200        210        220        230        240 
FLVPSRMHPG KFYALPQAPQ QFKQLLMVSG FDRYFQIAPC FRDEDARADR SPGEFYQLDV 

       250        260        270        280        290        300 
EMSFVTQEDI FSTIEPVMYD LFTKFTAKKV SETPFVRIPY NESMLKYGSD KPDLRNPIII 

       310        320        330        340        350        360 
ADVTEIFRDS DFTIFRENIK KGSIVRAIPA PKAAALPRSF FDKMIEFAIS EGAGGLGYIQ 

       370        380        390        400        410        420 
FSETGEAKGP VAKFLSPQQL ESLKATASIS NGDAVFFASD KKEKAAKLAG KVRIRLGEEL 

       430        440        450        460        470        480 
DLLEKDCFKF CWITDFPFYE LNEDTGKIDF SHNPFSMPQG GLEALEQAKT TEELLELTAY 

       490        500        510        520        530        540 
QYDIVCNGIE LSSGAIRNHK PEIMYKAFSM AGYSEEEVDK RFGGMIRAFK FGAPPHGGIA 

       550        560        570        580        590        600 
PGIDRIVMLL AEATNIREII AFPLNQQAED LLMNAPSYVE DKALKELSIM LSPSILKNMK

« Hide

References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000053 Genomic DNA. Translation: AAY61989.1.
RefSeqYP_247154.1. NC_007109.1.

3D structure databases

ProteinModelPortalQ4UKE2.
ModBaseSearch...

Protein-protein interaction databases

STRING315456.RF_1138.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY61989; AAY61989; RF_1138.
GeneID3401435.
KEGGrfe:RF_1138.
PATRIC17893120. VBIRicFel64634_1374.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0173.
HOGENOMHOG000275160.
KOK01876.
OMAYQLDVEM.
ProtClustDBPRK00476.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
3.30.1360.30. 1 hit.
HAMAPMF_00044_B. Asp_tRNA_synth_B.
InterProIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA-helicase.
[Graphical view]
PANTHERPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF5. PTHR22594:SF5. 1 hit.
PfamPF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti. 1 hit.
[Graphical view]
PRINTSPR01042. TRNASYNTHASP.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsTIGR00459. aspS_bact. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYD_RICFE
AccessionPrimary (citable) accession number: Q4UKE2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: July 5, 2005
Last modified: May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names

SIMILARITY comments

Index of protein domains and families

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