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Q4UKE2

- SYDND_RICFE

UniProt

Q4UKE2 - SYDND_RICFE

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Protein

Aspartate--tRNA(Asp/Asn) ligase

Gene

aspS

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).UniRule annotation

Catalytic activityi

ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei33 – 331Important for tRNA non-discriminationUniRule annotation
Binding sitei176 – 1761AspartateUniRule annotation
Binding sitei222 – 2221AspartateUniRule annotation
Binding sitei452 – 4521AspartateUniRule annotation
Binding sitei490 – 4901ATPUniRule annotation
Binding sitei493 – 4931AspartateUniRule annotation
Binding sitei497 – 4971AspartateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi222 – 2243ATPUniRule annotation
Nucleotide bindingi542 – 5454ATPUniRule annotation

GO - Molecular functioni

  1. aspartate-tRNA(Asn) ligase activity Source: UniProtKB-EC
  2. aspartate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. ATP binding Source: UniProtKB-HAMAP
  4. nucleic acid binding Source: InterPro

GO - Biological processi

  1. aspartyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-1171-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA(Asp/Asn) ligaseUniRule annotation (EC:6.1.1.23UniRule annotation)
Alternative name(s):
Aspartyl-tRNA synthetaseUniRule annotation
Short name:
AspRSUniRule annotation
Non-discriminating aspartyl-tRNA synthetaseUniRule annotation
Short name:
ND-AspRSUniRule annotation
Gene namesi
Name:aspSUniRule annotation
Ordered Locus Names:RF_1138
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000008548: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 600600Aspartate--tRNA(Asp/Asn) ligasePRO_0000235553Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi315456.RF_1138.

Structurei

3D structure databases

ProteinModelPortaliQ4UKE2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni200 – 2034AspartateUniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0173.
HOGENOMiHOG000275160.
KOiK01876.
OMAiSLSRKQI.
OrthoDBiEOG68Q0NX.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.1360.30. 1 hit.
HAMAPiMF_00044. Asp_tRNA_synth.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF5. PTHR22594:SF5. 1 hit.
PfamiPF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4UKE2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHKYRTHNCN ELQISDVRKE VKLSGWVHRR RDHGNLVFID LRDHYGITQI
60 70 80 90 100
VFTDQNQQLM EDASRLRYES VITVRGTIVA RSNDTINDTL PTGHIEVLAG
110 120 130 140 150
EFIVESAADT LPFVINTEKD APEETRLKHR FLDLRREKLH NNIILRSQII
160 170 180 190 200
SHIRHLMTGR GFTEFQTPIL TASSPEGARD FLVPSRMHPG KFYALPQAPQ
210 220 230 240 250
QFKQLLMVSG FDRYFQIAPC FRDEDARADR SPGEFYQLDV EMSFVTQEDI
260 270 280 290 300
FSTIEPVMYD LFTKFTAKKV SETPFVRIPY NESMLKYGSD KPDLRNPIII
310 320 330 340 350
ADVTEIFRDS DFTIFRENIK KGSIVRAIPA PKAAALPRSF FDKMIEFAIS
360 370 380 390 400
EGAGGLGYIQ FSETGEAKGP VAKFLSPQQL ESLKATASIS NGDAVFFASD
410 420 430 440 450
KKEKAAKLAG KVRIRLGEEL DLLEKDCFKF CWITDFPFYE LNEDTGKIDF
460 470 480 490 500
SHNPFSMPQG GLEALEQAKT TEELLELTAY QYDIVCNGIE LSSGAIRNHK
510 520 530 540 550
PEIMYKAFSM AGYSEEEVDK RFGGMIRAFK FGAPPHGGIA PGIDRIVMLL
560 570 580 590 600
AEATNIREII AFPLNQQAED LLMNAPSYVE DKALKELSIM LSPSILKNMK
Length:600
Mass (Da):67,976
Last modified:July 5, 2005 - v1
Checksum:i4FF6DFE4682E5264
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61989.1.
RefSeqiYP_247154.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY61989; AAY61989; RF_1138.
GeneIDi3401435.
KEGGirfe:RF_1138.
PATRICi17893120. VBIRicFel64634_1374.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61989.1 .
RefSeqi YP_247154.1. NC_007109.1.

3D structure databases

ProteinModelPortali Q4UKE2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 315456.RF_1138.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY61989 ; AAY61989 ; RF_1138 .
GeneIDi 3401435.
KEGGi rfe:RF_1138.
PATRICi 17893120. VBIRicFel64634_1374.

Phylogenomic databases

eggNOGi COG0173.
HOGENOMi HOG000275160.
KOi K01876.
OMAi SLSRKQI.
OrthoDBi EOG68Q0NX.

Enzyme and pathway databases

BioCyci RFEL315456:GKEK-1171-MONOMER.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
3.30.1360.30. 1 hit.
HAMAPi MF_00044. Asp_tRNA_synth.
InterProi IPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view ]
PANTHERi PTHR22594. PTHR22594. 1 hit.
PTHR22594:SF5. PTHR22594:SF5. 1 hit.
Pfami PF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view ]
PRINTSi PR01042. TRNASYNTHASP.
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsi TIGR00459. aspS_bact. 1 hit.
PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.

Entry informationi

Entry nameiSYDND_RICFE
AccessioniPrimary (citable) accession number: Q4UKE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: July 5, 2005
Last modified: October 29, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3