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Q4UKE2

- SYDND_RICFE

UniProt

Q4UKE2 - SYDND_RICFE

Protein

Aspartate--tRNA(Asp/Asn) ligase

Gene

aspS

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (05 Jul 2005)
      Previous versions | rss
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    Functioni

    Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).UniRule annotation

    Catalytic activityi

    ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei33 – 331Important for tRNA non-discriminationUniRule annotation
    Binding sitei176 – 1761AspartateUniRule annotation
    Binding sitei222 – 2221AspartateUniRule annotation
    Binding sitei452 – 4521AspartateUniRule annotation
    Binding sitei490 – 4901ATPUniRule annotation
    Binding sitei493 – 4931AspartateUniRule annotation
    Binding sitei497 – 4971AspartateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi222 – 2243ATPUniRule annotation
    Nucleotide bindingi542 – 5454ATPUniRule annotation

    GO - Molecular functioni

    1. aspartate-tRNA(Asn) ligase activity Source: UniProtKB-EC
    2. aspartate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. ATP binding Source: UniProtKB-HAMAP
    4. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. aspartyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciRFEL315456:GKEK-1171-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aspartate--tRNA(Asp/Asn) ligaseUniRule annotation (EC:6.1.1.23UniRule annotation)
    Alternative name(s):
    Aspartyl-tRNA synthetaseUniRule annotation
    Short name:
    AspRSUniRule annotation
    Non-discriminating aspartyl-tRNA synthetaseUniRule annotation
    Short name:
    ND-AspRSUniRule annotation
    Gene namesi
    Name:aspSUniRule annotation
    Ordered Locus Names:RF_1138
    OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
    Taxonomic identifieri315456 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
    ProteomesiUP000008548: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 600600Aspartate--tRNA(Asp/Asn) ligasePRO_0000235553Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi315456.RF_1138.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4UKE2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni200 – 2034AspartateUniRule annotation

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0173.
    HOGENOMiHOG000275160.
    KOiK01876.
    OMAiSLSRKQI.
    OrthoDBiEOG68Q0NX.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.30.1360.30. 1 hit.
    HAMAPiMF_00044. Asp_tRNA_synth.
    InterProiIPR004364. aa-tRNA-synt_II.
    IPR018150. aa-tRNA-synt_II-like.
    IPR006195. aa-tRNA-synth_II.
    IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
    IPR002312. Asp/Asn-tRNA-synth_IIb.
    IPR004115. GAD-like.
    IPR029351. GAD_dom.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    [Graphical view]
    PANTHERiPTHR22594. PTHR22594. 1 hit.
    PTHR22594:SF5. PTHR22594:SF5. 1 hit.
    PfamiPF02938. GAD. 1 hit.
    PF00152. tRNA-synt_2. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view]
    PRINTSiPR01042. TRNASYNTHASP.
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF55261. SSF55261. 1 hit.
    TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4UKE2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHKYRTHNCN ELQISDVRKE VKLSGWVHRR RDHGNLVFID LRDHYGITQI    50
    VFTDQNQQLM EDASRLRYES VITVRGTIVA RSNDTINDTL PTGHIEVLAG 100
    EFIVESAADT LPFVINTEKD APEETRLKHR FLDLRREKLH NNIILRSQII 150
    SHIRHLMTGR GFTEFQTPIL TASSPEGARD FLVPSRMHPG KFYALPQAPQ 200
    QFKQLLMVSG FDRYFQIAPC FRDEDARADR SPGEFYQLDV EMSFVTQEDI 250
    FSTIEPVMYD LFTKFTAKKV SETPFVRIPY NESMLKYGSD KPDLRNPIII 300
    ADVTEIFRDS DFTIFRENIK KGSIVRAIPA PKAAALPRSF FDKMIEFAIS 350
    EGAGGLGYIQ FSETGEAKGP VAKFLSPQQL ESLKATASIS NGDAVFFASD 400
    KKEKAAKLAG KVRIRLGEEL DLLEKDCFKF CWITDFPFYE LNEDTGKIDF 450
    SHNPFSMPQG GLEALEQAKT TEELLELTAY QYDIVCNGIE LSSGAIRNHK 500
    PEIMYKAFSM AGYSEEEVDK RFGGMIRAFK FGAPPHGGIA PGIDRIVMLL 550
    AEATNIREII AFPLNQQAED LLMNAPSYVE DKALKELSIM LSPSILKNMK 600
    Length:600
    Mass (Da):67,976
    Last modified:July 5, 2005 - v1
    Checksum:i4FF6DFE4682E5264
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000053 Genomic DNA. Translation: AAY61989.1.
    RefSeqiYP_247154.1. NC_007109.1.

    Genome annotation databases

    EnsemblBacteriaiAAY61989; AAY61989; RF_1138.
    GeneIDi3401435.
    KEGGirfe:RF_1138.
    PATRICi17893120. VBIRicFel64634_1374.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000053 Genomic DNA. Translation: AAY61989.1 .
    RefSeqi YP_247154.1. NC_007109.1.

    3D structure databases

    ProteinModelPortali Q4UKE2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 315456.RF_1138.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY61989 ; AAY61989 ; RF_1138 .
    GeneIDi 3401435.
    KEGGi rfe:RF_1138.
    PATRICi 17893120. VBIRicFel64634_1374.

    Phylogenomic databases

    eggNOGi COG0173.
    HOGENOMi HOG000275160.
    KOi K01876.
    OMAi SLSRKQI.
    OrthoDBi EOG68Q0NX.

    Enzyme and pathway databases

    BioCyci RFEL315456:GKEK-1171-MONOMER.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.30.1360.30. 1 hit.
    HAMAPi MF_00044. Asp_tRNA_synth.
    InterProi IPR004364. aa-tRNA-synt_II.
    IPR018150. aa-tRNA-synt_II-like.
    IPR006195. aa-tRNA-synth_II.
    IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
    IPR002312. Asp/Asn-tRNA-synth_IIb.
    IPR004115. GAD-like.
    IPR029351. GAD_dom.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    [Graphical view ]
    PANTHERi PTHR22594. PTHR22594. 1 hit.
    PTHR22594:SF5. PTHR22594:SF5. 1 hit.
    Pfami PF02938. GAD. 1 hit.
    PF00152. tRNA-synt_2. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view ]
    PRINTSi PR01042. TRNASYNTHASP.
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF55261. SSF55261. 1 hit.
    TIGRFAMsi TIGR00459. aspS_bact. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
      Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
      PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC VR-1525 / URRWXCal2.

    Entry informationi

    Entry nameiSYDND_RICFE
    AccessioniPrimary (citable) accession number: Q4UKE2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2006
    Last sequence update: July 5, 2005
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Rickettsia felis (Rickettsia azadi)
      Rickettsia felis (strain URRWXCal2): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3