Q4UKB0 (ERA_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTPase Era

Gene names

Name:	era
Ordered Locus Names:	RF_1174

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism By similarity. HAMAP-Rule MF_00367
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. Cell inner membrane; Peripheral membrane protein By similarity HAMAP-Rule MF_00367.
Sequence similarities	Belongs to the Era/MnmE GTP-binding protein family. Era subfamily. Contains 1 G (guanine nucleotide-binding) domain. Contains 1 KH type-2 domain.

Ontologies

Keywords
Biological process	Ribosome biogenesis
Cellular component	Cell inner membrane Cell membrane Cytoplasm Membrane
Ligand	GTP-binding Nucleotide-binding RNA-binding rRNA-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC ribosomal small subunit biogenesis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: HAMAP SSU rRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	GTPase Era HAMAP-Rule MF_00367		PRO_0000277965
Regions
	Domain	18 – 123	106	G
	Domain	202 – 279	78	KH type-2
	Nucleotide binding	13 – 20	8	GTP Potential
	Nucleotide binding	60 – 64	5	GTP Potential
	Nucleotide binding	122 – 125	4	GTP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4UKB0 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 82D1B619DA0DEE00	FASTA	293	33,694
	10 20 30 40 50 60 MTKQKTISFC IIGRPNSGKS TLLNRIIGEK LSIVTPKVQT TRSIITGIIT LKDTQVILYD 70 80 90 100 110 120 TPGIFEPKGT LEKAMVRCAW SSLHSADLVM LIIDSLKPFD DVMHDILDKL RSLNIVPIFL 130 140 150 160 170 180 LNKIDIESKY LDDIKAFLTE NHPDSLLFPI SALSGKNIDG LLEYITGKAK ISPWLYAEDD 190 200 210 220 230 240 ITDLPMRFIA AEITREQLFF NLQQELPYKL TVQTEKWEVL KDKSIKINQV IVVSRESYKT 250 260 270 280 290 IILGKNGSKI KEIGAKSRMQ MERFFGFPVH LFLFVKVREL WENNQEFYQY MKI

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY62025.1.
RefSeq	YP_247190.1. NC_007109.1.
3D structure databases
HSSP	HSSP built from PDB template 1MKY based on UniProtKB Q9X1F8.
ProteinModelPortal	Q4UKB0.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_1174.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY62025; AAY62025; RF_1174.
GeneID	3401472.
KEGG	rfe:RF_1174.
PATRIC	17893202. VBIRicFel64634_1414.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1159.
HOGENOM	HOG000245597.
KO	K03595.
OMA	ITREQLF.
ProtClustDB	PRK00089.
Family and domain databases
Gene3D	3.30.300.20. 1 hit.
HAMAP	MF_00367. GTPase_Era.
InterPro	IPR005662. GTP-bd_Era. IPR006073. GTP_binding_domain. IPR015946. KH_dom-like_a/b. IPR004044. KH_dom_type_2. IPR009019. KH_prok-type. IPR005225. Small_GTP-bd_dom. [Graphical view]
Pfam	PF07650. KH_2. 1 hit. PF01926. MMR_HSR1. 1 hit. [Graphical view]
SUPFAM	SSF54814. KH_prok. 1 hit.
TIGRFAMs	TIGR00436. era. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS50823. KH_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERA_RICFE

Accession

Primary (citable) accession number: Q4UKB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	July 5, 2005
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program