Q4UKA7 (LEP_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal peptidase I
Short name=SPase I
EC=3.4.21.89

Alternative name(s):
Leader peptidase I

Gene names

Name:	lepB
Ordered Locus Names:	RF_1177

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell inner membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	Signal peptidase I		PRO_0000316275
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 41	21	Helical; Potential
	Topological domain	42 – 266	225	Periplasmic Potential
Sites
	Active site	45	1	By similarity
	Active site	108	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UKA7 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: D7CE673D20F67D45	FASTA	266	31,119
	10 20 30 40 50 60 MQTDNTKSNT NKTAKQEWGS FAFVICIALL IRILIMEPFT VPTGSMKATI LENDYIFSTK 70 80 90 100 110 120 YSYGYSNYSL SFFDFIPLFK GRIFAREPER GDIVVFRPPN DMNVRYIKRL IGLPGDKIQL 130 140 150 160 170 180 IDDVIYINDK KIERTEVGTY ISEEGIKYLK FKETLPNGRT YFSYKLAPIF GVIYNDRYGN 190 200 210 220 230 240 TDVFYVPEGK YFFLGDNRDQ SNDSRVNLGF VPFENFIAKA QFIWLSTKIT WWDNDIGVIN 250 260 LVLKLKPWIE SVRLNRIFRN LYSTDE

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY62028.1.
RefSeq	YP_247193.1. NC_007109.1.
3D structure databases
ProteinModelPortal	Q4UKA7.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_1177.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY62028; AAY62028; RF_1177.
GeneID	3401475.
KEGG	rfe:RF_1177.
PATRIC	17893208. VBIRicFel64634_1417.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0681.
HOGENOM	HOG000003673.
KO	K03100.
OMA	KPWIESV.
ProtClustDB	CLSK870751.
Enzyme and pathway databases
BioCyc	RFEL315456:GKEK-1211-MONOMER.
Family and domain databases
Gene3D	2.10.109.10. 2 hits.
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019757. Pept_S26A_signal_pept_1_Lys-AS. IPR019759. Peptidase_S24_S26. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view]
PANTHER	PTHR12383. PTHR12383. 1 hit.
Pfam	PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
SUPFAM	SSF51306. Pept_S24_S26_C. 1 hit.
TIGRFAMs	TIGR02227. sigpep_I_bact. 1 hit.
PROSITE	PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP_RICFE

Accession

Primary (citable) accession number: Q4UKA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	July 5, 2005
Last modified:	May 29, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program