Reviewed, UniProtKB/Swiss-Prot Q4UKA6 (NUOF_RICFE)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit F
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit F
    NDH-1 subunit F

Gene names

Name:	nuoF
Ordered Locus Names:	RF_1178

Organism

Rickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

42862 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Cofactor	Binds 1 FMN Potential. Binds 1 4Fe-4S cluster Potential.
Sequence similarities	Belongs to the complex I 51 kDa subunit family.

Ontologies

Keywords
Ligand	4Fe-4S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FMN binding Inferred from electronic annotation. Source: InterPro NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	NADH-quinone oxidoreductase subunit F		PRO_0000274788
Regions
	Nucleotide binding	54 – 63	10	NAD By similarity
	Nucleotide binding	166 – 213	48	FMN By similarity
Sites
	Metal binding	344	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	347	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	350	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	390	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4UKA6-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 7DDC06F6B836DC5E	FASTA	422	46,224
	10 20 30 40 50 60 MLKEEDKIFT NLHGQQSHDL KSSKKRGDWD NTKALLDKGR EFIIEEVKKS GLRGRGGAGF 70 80 90 100 110 120 STGMKWSFMP KNSAKPCYLV VNADESEPGT CKDRDILRFE PHKLIEGCLL ASFAIGANDC 130 140 150 160 170 180 YIYIRGEFYN EASNIQRALD EAYKDGLIGK NACGSGFDCN IYLHRGAGAY ICGEETALLE 190 200 210 220 230 240 SLEGKKGMPR LKPPFPAGFG LYGCPTTINN VESIAVVPTI LRRGASWFAS IGKPNNTGTK 250 260 270 280 290 300 IFCISGHVNK PCNVEEAMGI SLKELIEKYA GGVRGGWDNL KAIIPGGSSV PLLPKSLCEV 310 320 330 340 350 360 DMDFDSLRTA GSGLGTGGII VMDKSTDIIY AIARLSKFYM HESCGQCTPC REGTGWMWRV 370 380 390 400 410 420 MMRLVKGNAQ KSEIDELLNV TKEIEGHTIC ALGDAAAWPI QGLIRHFRSE IEERIKGWSS AI

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000053 Genomic DNA. Translation: AAY62029.1.
RefSeq	YP_247194.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3401476.
GenomeReviews	Gene locus RF_1178 in contig CP000053_GR.
KEGG	rfe:RF_1178.
NMPDR	fig|315456.3.peg.1178.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4UKA6.
OMA	Q4UKA6. AMPVRAM.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_1178-MON.
BRENDA	1.6.99.5. 297363.
Family and domain databases
InterPro	IPR001949. NADH-UbQ_OxRdtase_51KDa_CS. IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd. IPR011537. NADH-UbQ_OxRdtase_suF. IPR011538. NADH_UbQ_OxRdtase_51KDa_su. IPR019554. Soluble_ligand_bd. [Graphical view]
Pfam	PF01512. Complex1_51K. 1 hit. PF10589. NADH_4Fe-4S. 1 hit. PF10531. SLBB. 1 hit. [Graphical view]
TIGRFAMs	TIGR01959. nuoF_fam. 1 hit.
PROSITE	PS00644. COMPLEX1_51K_1. 1 hit. PS00645. COMPLEX1_51K_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOF_RICFE

Accession

Primary (citable) accession number: Q4UKA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	July 5, 2005
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)