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Protein

NADH-quinone oxidoreductase subunit F

Gene

nuoF

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).By similarity

Catalytic activityi

NADH + quinone = NAD+ + quinol.

Cofactori

Protein has several cofactor binding sites:
  • FMNCuratedNote: Binds 1 FMN.Curated
  • [4Fe-4S] clusterCuratedNote: Binds 1 [4Fe-4S] cluster.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi344 – 3441Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi347 – 3471Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi350 – 3501Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi390 – 3901Iron-sulfur (4Fe-4S)Sequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi54 – 6310NADBy similarity
Nucleotide bindingi166 – 21348FMNBy similarityAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-1212-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit F (EC:1.6.99.5)
Alternative name(s):
NADH dehydrogenase I subunit F
NDH-1 subunit F
Gene namesi
Name:nuoF
Ordered Locus Names:RF_1178
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000008548 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 422422NADH-quinone oxidoreductase subunit FPRO_0000274788Add
BLAST

Keywords - PTMi

Quinone

Interactioni

Protein-protein interaction databases

STRINGi315456.RF_1178.

Structurei

3D structure databases

ProteinModelPortaliQ4UKA6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 51 kDa subunit family.Curated

Phylogenomic databases

eggNOGiCOG1894.
HOGENOMiHOG000251534.
KOiK00335.
OMAiATFAMGA.
OrthoDBiEOG6W9XGK.

Family and domain databases

InterProiIPR001949. NADH-UbQ_OxRdtase_51kDa_CS.
IPR011537. NADH-UbQ_OxRdtase_suF.
IPR011538. Nuo51_FMN-bd.
IPR019575. Nuop51_4Fe4S-bd.
IPR019554. Soluble_ligand-bd.
[Graphical view]
PfamiPF01512. Complex1_51K. 1 hit.
PF10589. NADH_4Fe-4S. 1 hit.
PF10531. SLBB. 1 hit.
[Graphical view]
SMARTiSM00928. NADH_4Fe-4S. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01959. nuoF_fam. 1 hit.
PROSITEiPS00644. COMPLEX1_51K_1. 1 hit.
PS00645. COMPLEX1_51K_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4UKA6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKEEDKIFT NLHGQQSHDL KSSKKRGDWD NTKALLDKGR EFIIEEVKKS
60 70 80 90 100
GLRGRGGAGF STGMKWSFMP KNSAKPCYLV VNADESEPGT CKDRDILRFE
110 120 130 140 150
PHKLIEGCLL ASFAIGANDC YIYIRGEFYN EASNIQRALD EAYKDGLIGK
160 170 180 190 200
NACGSGFDCN IYLHRGAGAY ICGEETALLE SLEGKKGMPR LKPPFPAGFG
210 220 230 240 250
LYGCPTTINN VESIAVVPTI LRRGASWFAS IGKPNNTGTK IFCISGHVNK
260 270 280 290 300
PCNVEEAMGI SLKELIEKYA GGVRGGWDNL KAIIPGGSSV PLLPKSLCEV
310 320 330 340 350
DMDFDSLRTA GSGLGTGGII VMDKSTDIIY AIARLSKFYM HESCGQCTPC
360 370 380 390 400
REGTGWMWRV MMRLVKGNAQ KSEIDELLNV TKEIEGHTIC ALGDAAAWPI
410 420
QGLIRHFRSE IEERIKGWSS AI
Length:422
Mass (Da):46,224
Last modified:July 5, 2005 - v1
Checksum:i7DDC06F6B836DC5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY62029.1.
RefSeqiWP_011271486.1. NC_007109.1.
YP_247194.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY62029; AAY62029; RF_1178.
KEGGirfe:RF_1178.
PATRICi17893210. VBIRicFel64634_1418.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY62029.1.
RefSeqiWP_011271486.1. NC_007109.1.
YP_247194.1. NC_007109.1.

3D structure databases

ProteinModelPortaliQ4UKA6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi315456.RF_1178.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY62029; AAY62029; RF_1178.
KEGGirfe:RF_1178.
PATRICi17893210. VBIRicFel64634_1418.

Phylogenomic databases

eggNOGiCOG1894.
HOGENOMiHOG000251534.
KOiK00335.
OMAiATFAMGA.
OrthoDBiEOG6W9XGK.

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-1212-MONOMER.

Family and domain databases

InterProiIPR001949. NADH-UbQ_OxRdtase_51kDa_CS.
IPR011537. NADH-UbQ_OxRdtase_suF.
IPR011538. Nuo51_FMN-bd.
IPR019575. Nuop51_4Fe4S-bd.
IPR019554. Soluble_ligand-bd.
[Graphical view]
PfamiPF01512. Complex1_51K. 1 hit.
PF10589. NADH_4Fe-4S. 1 hit.
PF10531. SLBB. 1 hit.
[Graphical view]
SMARTiSM00928. NADH_4Fe-4S. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01959. nuoF_fam. 1 hit.
PROSITEiPS00644. COMPLEX1_51K_1. 1 hit.
PS00645. COMPLEX1_51K_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.

Entry informationi

Entry nameiNUOF_RICFE
AccessioniPrimary (citable) accession number: Q4UKA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: July 5, 2005
Last modified: May 27, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.