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Protein

NADH-quinone oxidoreductase subunit F

Gene

nuoF

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).By similarity

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

Cofactori

Protein has several cofactor binding sites:
  • FMNCuratedNote: Binds 1 FMN.Curated
  • [4Fe-4S] clusterCuratedNote: Binds 1 [4Fe-4S] cluster.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi344Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi347Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi350Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi390Iron-sulfur (4Fe-4S)Sequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi54 – 63NADBy similarity10
Nucleotide bindingi166 – 213FMNBy similarityAdd BLAST48

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
Ligand4Fe-4S, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit F (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I subunit F
NDH-1 subunit F
Gene namesi
Name:nuoF
Ordered Locus Names:RF_1178
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
Proteomesi
  • UP000008548 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002747881 – 422NADH-quinone oxidoreductase subunit FAdd BLAST422

Keywords - PTMi

Quinone

Interactioni

Protein-protein interaction databases

STRINGi315456.RF_1178

Structurei

3D structure databases

ProteinModelPortaliQ4UKA6
SMRiQ4UKA6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 51 kDa subunit family.Curated

Phylogenomic databases

eggNOGiENOG4107QIZ Bacteria
COG1894 LUCA
HOGENOMiHOG000251534
KOiK00335
OMAiCREGTDW
OrthoDBiPOG091H02IW

Family and domain databases

Gene3Di1.20.1440.230, 1 hit
3.40.50.11540, 1 hit
InterProiView protein in InterPro
IPR001949 NADH-UbQ_OxRdtase_51kDa_CS
IPR011537 NADH-UbQ_OxRdtase_suF
IPR011538 Nuo51_FMN-bd
IPR037225 Nuo51_FMN-bd_sf
IPR019575 Nuop51_4Fe4S-bd
IPR037207 Nuop51_4Fe4S-bd_sf
IPR019554 Soluble_ligand-bd
PfamiView protein in Pfam
PF01512 Complex1_51K, 1 hit
PF10589 NADH_4Fe-4S, 1 hit
PF10531 SLBB, 1 hit
SMARTiView protein in SMART
SM00928 NADH_4Fe-4S, 1 hit
SUPFAMiSSF140490 SSF140490, 1 hit
SSF142019 SSF142019, 1 hit
TIGRFAMsiTIGR01959 nuoF_fam, 1 hit
PROSITEiView protein in PROSITE
PS00644 COMPLEX1_51K_1, 1 hit
PS00645 COMPLEX1_51K_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q4UKA6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKEEDKIFT NLHGQQSHDL KSSKKRGDWD NTKALLDKGR EFIIEEVKKS
60 70 80 90 100
GLRGRGGAGF STGMKWSFMP KNSAKPCYLV VNADESEPGT CKDRDILRFE
110 120 130 140 150
PHKLIEGCLL ASFAIGANDC YIYIRGEFYN EASNIQRALD EAYKDGLIGK
160 170 180 190 200
NACGSGFDCN IYLHRGAGAY ICGEETALLE SLEGKKGMPR LKPPFPAGFG
210 220 230 240 250
LYGCPTTINN VESIAVVPTI LRRGASWFAS IGKPNNTGTK IFCISGHVNK
260 270 280 290 300
PCNVEEAMGI SLKELIEKYA GGVRGGWDNL KAIIPGGSSV PLLPKSLCEV
310 320 330 340 350
DMDFDSLRTA GSGLGTGGII VMDKSTDIIY AIARLSKFYM HESCGQCTPC
360 370 380 390 400
REGTGWMWRV MMRLVKGNAQ KSEIDELLNV TKEIEGHTIC ALGDAAAWPI
410 420
QGLIRHFRSE IEERIKGWSS AI
Length:422
Mass (Da):46,224
Last modified:July 5, 2005 - v1
Checksum:i7DDC06F6B836DC5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA Translation: AAY62029.1
RefSeqiWP_011271486.1, NC_007109.1

Genome annotation databases

EnsemblBacteriaiAAY62029; AAY62029; RF_1178
KEGGirfe:RF_1178

Similar proteinsi

Entry informationi

Entry nameiNUOF_RICFE
AccessioniPrimary (citable) accession number: Q4UKA6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: July 5, 2005
Last modified: March 28, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health