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Q4UK93

- END3_RICFE

UniProt

Q4UK93 - END3_RICFE

Protein

Endonuclease III

Gene

nth

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (05 Jul 2005)
      Previous versions | rss
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    Functioni

    DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

    Catalytic activityi

    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi187 – 1871Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi194 – 1941Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi197 – 1971Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi203 – 2031Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-HAMAP
    3. DNA binding Source: UniProtKB-HAMAP
    4. DNA N-glycosylase activity Source: UniProtKB-HAMAP
    5. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. base-excision repair Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Lyase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    4Fe-4S, DNA-binding, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciRFEL315456:GKEK-1225-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endonuclease IIIUniRule annotation (EC:4.2.99.18UniRule annotation)
    Alternative name(s):
    DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
    Gene namesi
    Name:nthUniRule annotation
    Ordered Locus Names:RF_1191
    OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
    Taxonomic identifieri315456 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
    ProteomesiUP000008548: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 213213Endonuclease IIIPRO_0000280932Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi315456.RF_1191.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4UK93.
    SMRiQ4UK93. Positions 1-209.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini108 – 12720HhHUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Nth/MutY family.UniRule annotation
    Contains 1 HhH domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0177.
    HOGENOMiHOG000252206.
    KOiK10773.
    OMAiDTHIYRV.
    OrthoDBiEOG6H4KC5.

    Family and domain databases

    Gene3Di1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    HAMAPiMF_00942. Nth.
    InterProiIPR011257. DNA_glycosylase.
    IPR004036. Endonuclease-III-like_CS2.
    IPR003651. Endouclease3_FeS-loop_motif.
    IPR003265. HhH-GPD_domain.
    IPR000445. HhH_motif.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR023170. HTH_base_excis_C.
    IPR005759. Nth.
    [Graphical view]
    PfamiPF10576. EndIII_4Fe-2S. 1 hit.
    PF00633. HHH. 1 hit.
    PF00730. HhH-GPD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001435. Nth. 1 hit.
    SMARTiSM00478. ENDO3c. 1 hit.
    SM00525. FES. 1 hit.
    SM00278. HhH1. 1 hit.
    [Graphical view]
    SUPFAMiSSF48150. SSF48150. 1 hit.
    TIGRFAMsiTIGR01083. nth. 1 hit.
    PROSITEiPS01155. ENDONUCLEASE_III_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4UK93-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQAQIVNKIF EIFSKNNPSP KTELIYKNDF TLLVAVMLSA QATDISVNLA    50
    TKSLFETYDT TEKILELGED GLKKYIKSIG LFNSKAKNII ALCKILISNY 100
    QSSVPNDFKE LIKLPGVGRK TANVVLNCLF GMPTMAVDTH VFRVAKRIGL 150
    ARGNSPEIVE KELLQIINEK WLTHAHHWLI LHGRYICKAR KPDCDICPIK 200
    EYCEYYNSPI ISK 213
    Length:213
    Mass (Da):24,134
    Last modified:July 5, 2005 - v1
    Checksum:iA949EE1DCC334D0F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000053 Genomic DNA. Translation: AAY62042.1.
    RefSeqiYP_247207.1. NC_007109.1.

    Genome annotation databases

    EnsemblBacteriaiAAY62042; AAY62042; RF_1191.
    GeneIDi3401489.
    KEGGirfe:RF_1191.
    PATRICi17893240. VBIRicFel64634_1433.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000053 Genomic DNA. Translation: AAY62042.1 .
    RefSeqi YP_247207.1. NC_007109.1.

    3D structure databases

    ProteinModelPortali Q4UK93.
    SMRi Q4UK93. Positions 1-209.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 315456.RF_1191.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY62042 ; AAY62042 ; RF_1191 .
    GeneIDi 3401489.
    KEGGi rfe:RF_1191.
    PATRICi 17893240. VBIRicFel64634_1433.

    Phylogenomic databases

    eggNOGi COG0177.
    HOGENOMi HOG000252206.
    KOi K10773.
    OMAi DTHIYRV.
    OrthoDBi EOG6H4KC5.

    Enzyme and pathway databases

    BioCyci RFEL315456:GKEK-1225-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    HAMAPi MF_00942. Nth.
    InterProi IPR011257. DNA_glycosylase.
    IPR004036. Endonuclease-III-like_CS2.
    IPR003651. Endouclease3_FeS-loop_motif.
    IPR003265. HhH-GPD_domain.
    IPR000445. HhH_motif.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR023170. HTH_base_excis_C.
    IPR005759. Nth.
    [Graphical view ]
    Pfami PF10576. EndIII_4Fe-2S. 1 hit.
    PF00633. HHH. 1 hit.
    PF00730. HhH-GPD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001435. Nth. 1 hit.
    SMARTi SM00478. ENDO3c. 1 hit.
    SM00525. FES. 1 hit.
    SM00278. HhH1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48150. SSF48150. 1 hit.
    TIGRFAMsi TIGR01083. nth. 1 hit.
    PROSITEi PS01155. ENDONUCLEASE_III_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
      Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
      PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC VR-1525 / URRWXCal2.

    Entry informationi

    Entry nameiEND3_RICFE
    AccessioniPrimary (citable) accession number: Q4UK93
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 2007
    Last sequence update: July 5, 2005
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Rickettsia felis (Rickettsia azadi)
      Rickettsia felis (strain URRWXCal2): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3