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Protein

DNA polymerase III subunit alpha

Gene

dnaE

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  1. 3'-5' exonuclease activity Source: InterPro
  2. DNA binding Source: InterPro
  3. DNA-directed DNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-1279-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III subunit alpha (EC:2.7.7.7)
Gene namesi
Name:dnaE
Ordered Locus Names:RF_1244
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000008548: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12071207DNA polymerase III subunit alphaPRO_0000280957Add
BLAST

Interactioni

Subunit structurei

DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex (By similarity).By similarity

Protein-protein interaction databases

STRINGi315456.RF_1244.

Structurei

3D structure databases

ProteinModelPortaliQ4UK40.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0587.
HOGENOMiHOG000021784.
KOiK02337.
OMAiMKFPGYM.
OrthoDBiEOG6CZQGR.

Family and domain databases

InterProiIPR011708. DNA_pol3_alpha.
IPR029460. DNAPol_HHH.
IPR004013. PHP_C.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
IPR004805. PolC_alpha.
[Graphical view]
PfamiPF07733. DNA_pol3_alpha. 1 hit.
PF14579. HHH_6. 1 hit.
PF02811. PHP. 1 hit.
[Graphical view]
SMARTiSM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMiSSF89550. SSF89550. 1 hit.
TIGRFAMsiTIGR00594. polc. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4UK40-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRPEFIHLRT QSSYSFLESA LTIEKVVELA SSNKMPAICL ADKGNLFGSL
60 70 80 90 100
EFALYAVKKG LQPIHGVILN IKYDIDIFAQ ILLIAKDETG YKNLLKLSSL
110 120 130 140 150
TFTKNDRKLC DHIDFEDLIE YQEGLIALCC YTDGIVGKCL LARSEEQAML
160 170 180 190 200
FARKLQDILG DRFYFEIMRH DLPEEQFIED SYIRIAAELA IPLVATNKVL
210 220 230 240 250
FSEKTMHDAH DVLLCISAGV TKEYPDRKTV SENCYFRSPH EMIELFSDLP
260 270 280 290 300
SAIQNTVNLR ERCYFAAHAN PPMLPNFATK DISETDLIRK DAKEGLLARL
310 320 330 340 350
ATKFKSENIP LQNQEELKTE YFARLNYELD IICNMNFAGY FLIVSDFIKW
360 370 380 390 400
SKKEGILVGP GRGSGAGSVV AWSLLITDLD PIKFGLLFER FLNPERISMP
410 420 430 440 450
DFDIDFCQER REEVINYVRS KYGNNRVGQI ITFGKMQAKA VIKDVARVLS
460 470 480 490 500
LPYKFADYLT ELVPFSAVNP VSLEQAMREV PELANAAKGN GLYNLEGEAE
510 520 530 540 550
LIKLVLDTSL ILEGLHRHSS THAAGIVIAG TDLVDIVPVY KDANSDMLVV
560 570 580 590 600
GYSMKYSEIA GLIKFDFLGL QTLTVITDCK KLLKEQGIEV DFNNMTFDDN
610 620 630 640 650
KTYQMLCKGK GVGVFQFESI GMKDALRRLK PDSIHDLIAL GALYRPGPME
660 670 680 690 700
NIPTYIACKH KLQQPDYLHE LLKPILEETY GVVIYQEQVQ RIAQILAGYT
710 720 730 740 750
LGAADLLRRA MGKKIKKEME EQEEIFVKGA IANNISESQA KSIFATVAKF
760 770 780 790 800
AGYGFNKAHA AAYGVISYQT AYLKANYPAE FLVACLNLEL NNHDKINLFL
810 820 830 840 850
QEAKDSGIKI IAPNINISEG YFSVKSVIPQ AATCHPQGPL CHPRVGGYPE
860 870 880 890 900
KVKTVLNHES MKMDSRFCGN DIKGSRNDIE DTGYDKEKST IIFALGAIKG
910 920 930 940 950
VTPNFGKLVT DERKARGAFK SITDFIERLP LKSINSKLLE NLIKSGCFDE
960 970 980 990 1000
LHDNRLQLFS SISKLLAYSA SYHAEQESNQ FSLIKVSSLS PNILIASDYA
1010 1020 1030 1040 1050
DNNTLAFYEF ESMGLFISNH PLTQYQEIFS RLNILNTADL HNNLPDGTNR
1060 1070 1080 1090 1100
VNLAGVIQKK DSRMSARGRF VTLVLSDPEN IFELSIFSEE VLKDYVHLLD
1110 1120 1130 1140 1150
VKSLVVVNCD IVKDEGGIKL TAKSFSSIED AINNKQFELQ LYPQNHEELR
1160 1170 1180 1190 1200
QIVTLLAART NNRDQSNAKA TIYLQSEGVK NFVAKITLPE KFFLQGQDFE

ILKGYSK
Length:1,207
Mass (Da):135,675
Last modified:July 5, 2005 - v1
Checksum:i061B7DD575BBDD96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY62095.1.
RefSeqiWP_011271544.1. NC_007109.1.
YP_247260.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY62095; AAY62095; RF_1244.
GeneIDi3401355.
KEGGirfe:RF_1244.
PATRICi17893368. VBIRicFel64634_1496.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY62095.1.
RefSeqiWP_011271544.1. NC_007109.1.
YP_247260.1. NC_007109.1.

3D structure databases

ProteinModelPortaliQ4UK40.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi315456.RF_1244.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY62095; AAY62095; RF_1244.
GeneIDi3401355.
KEGGirfe:RF_1244.
PATRICi17893368. VBIRicFel64634_1496.

Phylogenomic databases

eggNOGiCOG0587.
HOGENOMiHOG000021784.
KOiK02337.
OMAiMKFPGYM.
OrthoDBiEOG6CZQGR.

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-1279-MONOMER.

Family and domain databases

InterProiIPR011708. DNA_pol3_alpha.
IPR029460. DNAPol_HHH.
IPR004013. PHP_C.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
IPR004805. PolC_alpha.
[Graphical view]
PfamiPF07733. DNA_pol3_alpha. 1 hit.
PF14579. HHH_6. 1 hit.
PF02811. PHP. 1 hit.
[Graphical view]
SMARTiSM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMiSSF89550. SSF89550. 1 hit.
TIGRFAMsiTIGR00594. polc. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.

Entry informationi

Entry nameiDPO3A_RICFE
AccessioniPrimary (citable) accession number: Q4UK40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: July 5, 2005
Last modified: February 4, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.