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Q4UK40 (DPO3A_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase III subunit alpha

EC=2.7.7.7
Gene names
Name:dnaE
Ordered Locus Names:RF_1244
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length1207 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase By similarity.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Subunit structure

DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the DNA polymerase type-C family. DnaE subfamily.

Ontologies

Keywords
   Biological processDNA replication
   Cellular componentCytoplasm
   Molecular functionDNA-directed DNA polymerase
Nucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function3'-5' exonuclease activity

Inferred from electronic annotation. Source: InterPro

DNA binding

Inferred from electronic annotation. Source: InterPro

DNA-directed DNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12071207DNA polymerase III subunit alpha
PRO_0000280957

Sequences

Sequence LengthMass (Da)Tools
Q4UK40 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: 061B7DD575BBDD96

FASTA1,207135,675
        10         20         30         40         50         60 
MRPEFIHLRT QSSYSFLESA LTIEKVVELA SSNKMPAICL ADKGNLFGSL EFALYAVKKG 

        70         80         90        100        110        120 
LQPIHGVILN IKYDIDIFAQ ILLIAKDETG YKNLLKLSSL TFTKNDRKLC DHIDFEDLIE 

       130        140        150        160        170        180 
YQEGLIALCC YTDGIVGKCL LARSEEQAML FARKLQDILG DRFYFEIMRH DLPEEQFIED 

       190        200        210        220        230        240 
SYIRIAAELA IPLVATNKVL FSEKTMHDAH DVLLCISAGV TKEYPDRKTV SENCYFRSPH 

       250        260        270        280        290        300 
EMIELFSDLP SAIQNTVNLR ERCYFAAHAN PPMLPNFATK DISETDLIRK DAKEGLLARL 

       310        320        330        340        350        360 
ATKFKSENIP LQNQEELKTE YFARLNYELD IICNMNFAGY FLIVSDFIKW SKKEGILVGP 

       370        380        390        400        410        420 
GRGSGAGSVV AWSLLITDLD PIKFGLLFER FLNPERISMP DFDIDFCQER REEVINYVRS 

       430        440        450        460        470        480 
KYGNNRVGQI ITFGKMQAKA VIKDVARVLS LPYKFADYLT ELVPFSAVNP VSLEQAMREV 

       490        500        510        520        530        540 
PELANAAKGN GLYNLEGEAE LIKLVLDTSL ILEGLHRHSS THAAGIVIAG TDLVDIVPVY 

       550        560        570        580        590        600 
KDANSDMLVV GYSMKYSEIA GLIKFDFLGL QTLTVITDCK KLLKEQGIEV DFNNMTFDDN 

       610        620        630        640        650        660 
KTYQMLCKGK GVGVFQFESI GMKDALRRLK PDSIHDLIAL GALYRPGPME NIPTYIACKH 

       670        680        690        700        710        720 
KLQQPDYLHE LLKPILEETY GVVIYQEQVQ RIAQILAGYT LGAADLLRRA MGKKIKKEME 

       730        740        750        760        770        780 
EQEEIFVKGA IANNISESQA KSIFATVAKF AGYGFNKAHA AAYGVISYQT AYLKANYPAE 

       790        800        810        820        830        840 
FLVACLNLEL NNHDKINLFL QEAKDSGIKI IAPNINISEG YFSVKSVIPQ AATCHPQGPL 

       850        860        870        880        890        900 
CHPRVGGYPE KVKTVLNHES MKMDSRFCGN DIKGSRNDIE DTGYDKEKST IIFALGAIKG 

       910        920        930        940        950        960 
VTPNFGKLVT DERKARGAFK SITDFIERLP LKSINSKLLE NLIKSGCFDE LHDNRLQLFS 

       970        980        990       1000       1010       1020 
SISKLLAYSA SYHAEQESNQ FSLIKVSSLS PNILIASDYA DNNTLAFYEF ESMGLFISNH 

      1030       1040       1050       1060       1070       1080 
PLTQYQEIFS RLNILNTADL HNNLPDGTNR VNLAGVIQKK DSRMSARGRF VTLVLSDPEN 

      1090       1100       1110       1120       1130       1140 
IFELSIFSEE VLKDYVHLLD VKSLVVVNCD IVKDEGGIKL TAKSFSSIED AINNKQFELQ 

      1150       1160       1170       1180       1190       1200 
LYPQNHEELR QIVTLLAART NNRDQSNAKA TIYLQSEGVK NFVAKITLPE KFFLQGQDFE 


ILKGYSK 

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References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000053 Genomic DNA. Translation: AAY62095.1.
RefSeqYP_247260.1. NC_007109.1.

3D structure databases

ProteinModelPortalQ4UK40.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING315456.RF_1244.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY62095; AAY62095; RF_1244.
GeneID3401355.
KEGGrfe:RF_1244.
PATRIC17893368. VBIRicFel64634_1496.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0587.
HOGENOMHOG000021784.
KOK02337.
OMAFCMDGRD.
OrthoDBEOG6CZQGR.
ProtClustDBPRK05673.

Enzyme and pathway databases

BioCycRFEL315456:GKEK-1279-MONOMER.

Family and domain databases

InterProIPR011708. DNA_pol3_alpha.
IPR004013. PHP_C.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
IPR004805. PolC_alpha.
[Graphical view]
PfamPF07733. DNA_pol3_alpha. 1 hit.
PF02811. PHP. 1 hit.
[Graphical view]
SMARTSM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMSSF89550. SSF89550. 1 hit.
TIGRFAMsTIGR00594. polc. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDPO3A_RICFE
AccessionPrimary (citable) accession number: Q4UK40
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: July 5, 2005
Last modified: April 16, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names