Reviewed, UniProtKB/Swiss-Prot Q4UK22 (NUOG_RICFE)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit G
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit G
    NDH-1 subunit G

Gene names

Name:	nuoG
Ordered Locus Names:	RF_1262

Organism

Rickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

42862 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	671 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. Binds 2 4Fe-4S clusters per subunit By similarity.
Sequence similarities	Belongs to the complex I 75 kDa subunit family. Contains 1 2Fe-2S ferredoxin-type domain.

Ontologies

Keywords
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 671	671	NADH-quinone oxidoreductase subunit G		PRO_0000287843
Regions
	Domain	1 – 78	78	2Fe-2S ferredoxin-type
Sites
	Metal binding	34	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	45	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	48	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	62	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	94	1	Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
	Metal binding	98	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	101	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	107	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	146	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	149	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	152	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	196	1	Iron-sulfur 3 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UK22-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 8ABAC83059DD6C01	FASTA	671	74,861
	10 20 30 40 50 60 MIKLNIDGSE IEVSEGSTVY QACTQAGKEI PHFCYHERLK IAGNCRMCLV EMEKSPKPIA 70 80 90 100 110 120 SCAMPVSNGM VIHTDTPMVK KAREGVMEFL LINHPLDCPI CDQGGECDLQ DQAFRYGKGT 130 140 150 160 170 180 NRFHENKRSI KDKYMGPLIK TAMTRCIQCT RCIRFANDIA GIEEMGAIHR GEHMEVTSYL 190 200 210 220 230 240 EQTLDSEMSG NMIDICPVGA LNSKPYAFKA RKWELKHTAS IGVHDAEGSN IRIDSRGDEV 250 260 270 280 290 300 MRILPRVNEE INEEWLSDKN RFSYDGLKYQ RLDLPYIRKN GKLVEASWSE ALKTIADKIK 310 320 330 340 350 360 SVKPEKIAAI AGSLVSVEAM FMLKTLLQKL GSNNYSVNQF DYKFDTTQRG NYLFNTTIAG 370 380 390 400 410 420 VEKADLCLLI GANLRQIAPV LNSRIGQRVR AGSLKVARIG EGHNQTYRIQ DLGSDIKIIE 430 440 450 460 470 480 ELAIGTHEFT KALKAAKYPM IIVGDGVYAR DDGYAILSLI HKIVAEYNIM RDDFQGFNML 490 500 510 520 530 540 HNHASIVGGL DIGFNTPIKL EELELTYLLG ADELPFDKLK SAFIIYQGHH GDSGAANADV 550 560 570 580 590 600 ILPAAAYTEQ SGIYVNLEGR PQIAEKAVAP VGVAKEDIEI IKELAGSLKI DIGMDNLQEV 610 620 630 640 650 660 RVRLAKEYKI FANIDKIVES KFAKFSFKDK LSKEPITMGP INYYMTDVIS KNSVTMAKCV 670 EAKEKRNERA A

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000053 Genomic DNA. Translation: AAY62113.1.
RefSeq	YP_247278.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3401373.
GenomeReviews	Gene locus RF_1262 in contig CP000053_GR.
KEGG	rfe:RF_1262.
NMPDR	fig|315456.3.peg.1262.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4UK22.
OMA	Q4UK22. ARKWELR.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_1262-MON.
BRENDA	1.6.99.5. 297363.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR001041. Ferredoxin. IPR006656. Mopterin_OxRdtase. IPR000283. NADH_UbQ_OxRdtase_75KDa_su_CS. IPR010228. NADH_UbQ_OxRdtase_Gsu. IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd. IPR015405. NuoG_C. [Graphical view]
Pfam	PF09326. DUF1982. 1 hit. PF00111. Fer2. 1 hit. PF00384. Molybdopterin. 1 hit. PF10588. NADH-G_4Fe-4S_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01973. NuoG. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. False negative. PS51085. 2FE2S_FER_2. 1 hit. PS00641. COMPLEX1_75K_1. 1 hit. PS00642. COMPLEX1_75K_2. 1 hit. PS00643. COMPLEX1_75K_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOG_RICFE

Accession

Primary (citable) accession number: Q4UK22

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	July 5, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)