ID   ACON_RICFE              Reviewed;         878 AA.
AC   Q4UK20;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   16-JUN-2009, entry version 27.
DE   RecName: Full=Aconitate hydratase;
DE            Short=Aconitase;
DE            EC=4.2.1.3;
DE   AltName: Full=Citrate hydro-lyase;
GN   Name=acnA; OrderedLocusNames=RF_1264;
OS   Rickettsia felis (Rickettsia azadi).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX   NCBI_TaxID=42862;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC VR-1525 / URRWXCal2;
RX   PubMed=15984913; DOI=10.1371/journal.pbio.0030248;
RA   Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E.,
RA   Parinello H., Claverie J.-M., Raoult D.;
RT   "The genome sequence of Rickettsia felis identifies the first putative
RT   conjugative plasmid in an obligate intracellular parasite.";
RL   PLoS Biol. 3:1-12(2005).
CC   -!- CATALYTIC ACTIVITY: Citrate = isocitrate.
CC   -!- COFACTOR: Binds 1 4Fe-4S cluster per subunit (By similarity).
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle.
CC   -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
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DR   EMBL; CP000053; AAY62115.1; -; Genomic_DNA.
DR   RefSeq; YP_247280.1; -.
DR   GeneID; 3401375; -.
DR   GenomeReviews; CP000053_GR; RF_1264.
DR   KEGG; rfe:RF_1264; -.
DR   NMPDR; fig|315456.3.peg.1264; -.
DR   HOGENOM; Q4UK20; -.
DR   OMA; Q4UK20; RIKNEML.
DR   BioCyc; RFEL315456:RF_1264-MON; -.
DR   BRENDA; 4.2.1.3; 297363.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003994; F:aconitate hydratase activity; IEA:EC.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR   InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR   InterPro; IPR015937; Aconitase-like_core.
DR   InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR   InterPro; IPR006249; Aconitase/Fe_reg_prot_2.
DR   InterPro; IPR015934; Aconitase/Fe_reg_prot_2/AcnD.
DR   InterPro; IPR015932; Aconitase/IPMdHydase_lsu_aba_2.
DR   InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR   InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR   Gene3D; G3DSA:3.30.499.10; Acnase/IPM_dHydase_lsu_aba_1/3; 2.
DR   Gene3D; G3DSA:3.20.19.10; Aconitase/3IPM_dehydase_swvl; 1.
DR   Gene3D; G3DSA:3.40.1060.10; Aconitase/IPMdHydase_lsu_aba_2; 1.
DR   PANTHER; PTHR11670; Aconitase-like_core; 1.
DR   PANTHER; PTHR11670:SF1; Aconitase/Fe_reg_prot_2/AcnD; 1.
DR   Pfam; PF00330; Aconitase; 1.
DR   Pfam; PF00694; Aconitase_C; 1.
DR   PRINTS; PR00415; ACONITASE.
DR   ProDom; PD000511; Aconitase_N; 1.
DR   TIGRFAMs; TIGR01341; aconitase_1; 1.
DR   PROSITE; PS00450; ACONITASE_1; 1.
DR   PROSITE; PS01244; ACONITASE_2; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Complete proteome; Iron; Iron-sulfur; Lyase; Metal-binding;
KW   Tricarboxylic acid cycle.
FT   CHAIN         1    878       Aconitate hydratase.
FT                                /FTId=PRO_0000272343.
FT   METAL       426    426       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       492    492       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       495    495       Iron-sulfur (4Fe-4S) (By similarity).
SQ   SEQUENCE   878 AA;  97271 MW;  BAD15753404D3774 CRC64;
     MSKVHNSEYI KELSVDNTSY KIYDINKAAS DIELPLKKLP YSLRVLFENV LRSNGSKQNL
     LVFKEWLKTK ESDAEIDFMP ARVLMQDFTG VPAIVDLAAM RDAMKKIGGD PLKINPLIPV
     DLVIDHSVSV DSYAAKDSFD KNVQMEMKRN IERYQFLKWG QQAFNNFKVV PPGTGICHQV
     NLEYLAKVVW HKDGLAYPDS LVGTDSHTTM VNGLSVLGWG VGGIEAEAAM LGQPLTMILP
     EVIGVKLTGK LTGIATATDL VLTVTEMLRK KKVVGKFVEF FGEGLKNLTI ADRATISNMS
     PEYGATCGFF PIDQETIKYL ELTGREKTQI KLVEKYANEQ NLWYDFEHEA EYTEILELDL
     SMVHSSLAGP RRPQDRVDLN DVANNFKHEL PNFGIENKDI DKKYAVANQN YEIGNGDVVI
     AAITSCTNTS NPSVMIGAAL LAKKALEHGL KVKPWVKTSL APGSKVVTEY LKLSGLDKYL
     DELGFNLVGY GCTTCIGNSG PLNPEIEETI NKNGLVVASV LSGNRNFEGR INPLTKASYL
     GSPILVVAYA LSGTLNIDLN NQPIGKNIYL KDIWPSKEEI DEVIANSINS SMFIEKYSDI
     FSGTKEWKDL QITTSSTYNW NKNSTYINNP PYFEDIGSKN NIKDIKSAKI LAIFGDSITT
     DHISPAGSIS KTSPAAKYLT DNHIEPLDFN SYGSRRGNHE VMMRGTFANI RIKNEMCKGV
     EGGFTINQLS STQQTIYDAA MDYKANDVPV VIFAGKEYGS GSSRDWAAKG PQLLGVKAVI
     AESFERIHRS NLVGMGILPL TFTGNNTRLD LKLDGSETID IIGLSEQIKP YNPVKCMIKK
     QTGETRTIDL ILQIFTDNEI NYIKHGSIMH FVVENLKG
//