Reviewed, UniProtKB/Swiss-Prot Q4UK20 (ACON_RICFE)

Names and origin

Protein names

Recommended name:
    Aconitate hydratase
      Short name=Aconitase
    EC=4.2.1.3
Alternative name(s):
    Citrate hydro-lyase

Gene names

Name:	acnA
Ordered Locus Names:	RF_1264

Organism

Rickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

42862 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	878 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW aconitate hydratase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 878	878	Aconitate hydratase		PRO_0000272343
Sites
	Metal binding	426	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	492	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	495	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UK20-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: BAD15753404D3774	FASTA	878	97,271
	10 20 30 40 50 60 MSKVHNSEYI KELSVDNTSY KIYDINKAAS DIELPLKKLP YSLRVLFENV LRSNGSKQNL 70 80 90 100 110 120 LVFKEWLKTK ESDAEIDFMP ARVLMQDFTG VPAIVDLAAM RDAMKKIGGD PLKINPLIPV 130 140 150 160 170 180 DLVIDHSVSV DSYAAKDSFD KNVQMEMKRN IERYQFLKWG QQAFNNFKVV PPGTGICHQV 190 200 210 220 230 240 NLEYLAKVVW HKDGLAYPDS LVGTDSHTTM VNGLSVLGWG VGGIEAEAAM LGQPLTMILP 250 260 270 280 290 300 EVIGVKLTGK LTGIATATDL VLTVTEMLRK KKVVGKFVEF FGEGLKNLTI ADRATISNMS 310 320 330 340 350 360 PEYGATCGFF PIDQETIKYL ELTGREKTQI KLVEKYANEQ NLWYDFEHEA EYTEILELDL 370 380 390 400 410 420 SMVHSSLAGP RRPQDRVDLN DVANNFKHEL PNFGIENKDI DKKYAVANQN YEIGNGDVVI 430 440 450 460 470 480 AAITSCTNTS NPSVMIGAAL LAKKALEHGL KVKPWVKTSL APGSKVVTEY LKLSGLDKYL 490 500 510 520 530 540 DELGFNLVGY GCTTCIGNSG PLNPEIEETI NKNGLVVASV LSGNRNFEGR INPLTKASYL 550 560 570 580 590 600 GSPILVVAYA LSGTLNIDLN NQPIGKNIYL KDIWPSKEEI DEVIANSINS SMFIEKYSDI 610 620 630 640 650 660 FSGTKEWKDL QITTSSTYNW NKNSTYINNP PYFEDIGSKN NIKDIKSAKI LAIFGDSITT 670 680 690 700 710 720 DHISPAGSIS KTSPAAKYLT DNHIEPLDFN SYGSRRGNHE VMMRGTFANI RIKNEMCKGV 730 740 750 760 770 780 EGGFTINQLS STQQTIYDAA MDYKANDVPV VIFAGKEYGS GSSRDWAAKG PQLLGVKAVI 790 800 810 820 830 840 AESFERIHRS NLVGMGILPL TFTGNNTRLD LKLDGSETID IIGLSEQIKP YNPVKCMIKK 850 860 870 QTGETRTIDL ILQIFTDNEI NYIKHGSIMH FVVENLKG

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000053 Genomic DNA. Translation: AAY62115.1.
RefSeq	YP_247280.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3401375.
GenomeReviews	Gene locus RF_1264 in contig CP000053_GR.
KEGG	rfe:RF_1264.
NMPDR	fig|315456.3.peg.1264.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4UK20.
OMA	Q4UK20. RIKNEML.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_1264-MON.
BRENDA	4.2.1.3. 297363.
Family and domain databases
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015937. Aconitase-like_core. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR006249. Aconitase/Fe_reg_prot_2. IPR015934. Aconitase/Fe_reg_prot_2/AcnD. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
Gene3D	G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 2 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF1. Aconitase/Fe_reg_prot_2/AcnD. 1 hit.
Pfam	PF00330. Aconitase. 1 hit. PF00694. Aconitase_C. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
ProDom	PD000511. Aconitase_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01341. aconitase_1. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACON_RICFE

Accession

Primary (citable) accession number: Q4UK20

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	July 5, 2005
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)