Reviewed, UniProtKB/Swiss-Prot Q4UJV4 (HEM1_RICFE)

Names and origin

Protein names

Recommended name:
    5-aminolevulinate synthase
    EC=2.3.1.37
Alternative name(s):
    5-aminolevulinic acid synthase
    Delta-aminolevulinate synthase
    Delta-ALA synthetase

Gene names

Name:	hemA
Ordered Locus Names:	RF_1334

Organism

Rickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

42862 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
Sequence similarities	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Heme biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-aminolevulinate synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring nitrogenous groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 414	414	5-aminolevulinate synthase		PRO_0000280898
Amino acid modifications
	Modified residue	244	1	N6-(pyridoxal phosphate)lysine Probable

Sequences

	Sequence		Length	Mass (Da)
	Q4UJV4-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 6B7987FD78DEF8AB	FASTA	414	46,180
	10 20 30 40 50 60 MSYYDTIFSK HIDKIKSEGR YREFKALKRQ ADNFPFAEHA NKQIVMWCIN DYLGMSKHAK 70 80 90 100 110 120 VMQASIDALL KYGVGSGGTR NIGGNNIAIL ELEKELADLH KKQAALVFTS GFVANDTTLA 130 140 150 160 170 180 SLAKIMPDIV FFSDELNHAS IIAGVTSSRA EKYIYRHLDV KHLEELLQSV DINRPKIIVF 190 200 210 220 230 240 ESAYSMDGFF SPIKDIINLA KKYNALTFID EVHTVGLYGK HGGGIAELLN CSDQIDIIQG 250 260 270 280 290 300 TLAKAYGTIG GYITSNHNLV DAIRLTAPGF IFTTSLPPVI STAATHSIRH LKESNEERIK 310 320 330 340 350 360 HQEVVTKLKN SFENFNIPYL KNESHIVPII IGDPIKAASA SNMLLNKYGI YVQHINFPTV 370 380 390 400 410 PRGTERLRII PTPAHTDKMI NDLSIALVHI FAELDIELSS TKELNEEIRL NLIA

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000053 Genomic DNA. Translation: AAY62185.1.
RefSeq	YP_247350.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3400890.
GenomeReviews	Gene locus RF_1334 in contig CP000053_GR.
KEGG	rfe:RF_1334.
NMPDR	fig|315456.3.peg.1334.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4UJV4.
OMA	Q4UJV4. FSPIKDI.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_1334-MON.
BRENDA	2.3.1.37. 297363.
Family and domain databases
InterPro	IPR010961. 4pyrrol_synth_NH2levulA_synth. IPR004839. Aminotrans_I/II. IPR001917. Aminotrans_II_pyridoxalP_BS. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01821. 5aminolev_synth. 1 hit.
PROSITE	PS00599. AA_TRANSFER_CLASS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM1_RICFE

Accession

Primary (citable) accession number: Q4UJV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	July 5, 2005
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)