Q4UJV2 (TIG_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trigger factor
Short name=TF
EC=5.2.1.8

Alternative name(s):
PPIase

Gene names

Name:	tig
Ordered Locus Names:	RF_1336

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase By similarity. HAMAP-Rule MF_00303
Catalytic activity	Peptidylproline (omega=180) = peptidylproline (omega=0). HAMAP-Rule MF_00303
Subcellular location	Cytoplasm. Note: About half TF is bound to the ribosome near the polypeptide exit tunnel while the other half is free in the cytoplasm By similarity. HAMAP-Rule MF_00303
Domain	Consists of 3 domains; the N-terminus binds the ribosome, the middle domain has PPIase activity, while the C-terminus has intrinsic chaperone activity on its own By similarity. HAMAP-Rule MF_00303
Sequence similarities	Belongs to the FKBP-type PPIase family. Tig subfamily. Contains 1 PPIase FKBP-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Cytoplasm
Molecular function	Chaperone Isomerase Rotamase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW protein folding Inferred from electronic annotation. Source: HAMAP protein peptidyl-prolyl isomerization Inferred from electronic annotation. Source: GOC protein transport Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	peptidyl-prolyl cis-trans isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	Trigger factor HAMAP-Rule MF_00303		PRO_0000256609
Regions
	Domain	162 – 247	86	PPIase FKBP-type

Sequences

	Sequence		Length	Mass (Da)
	Q4UJV2 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 82982B6368A643DD	FASTA	445	50,878
	10 20 30 40 50 60 MGITVLKNEG LDFHARISTP LSEIDDDIQK ELLDLTKKVK IAGFRAGKVP VSIVKKKYGT 70 80 90 100 110 120 SVRNDIIERR INHSVNHVIK EHNLNIIGRP KIEELQNEPD KALEFTVKIE LLPKITIPDL 130 140 150 160 170 180 KKISLDRPKL EVNSKDVEEQ LEKLAALTKS YTKESKAKIK DGVQVTIDAI GYIKDEAFEG 190 200 210 220 230 240 GKLNDFKVVI GSNALIPGFE KQLIGSKTGS EVDVNVTFPE NYHAKDLAGK DARFAVQIKS 250 260 270 280 290 300 VHTAEPTVID EEFAKKFQSN SLEELRTHFA KQIENESEEA INTIMKMNLF DKLEKLLDFD 310 320 330 340 350 360 VPESLLEQEK NILKSETDKN DQDESLLKDK SPKEITEYYN KLALRRVRIG LLLAEYAKSK 370 380 390 400 410 420 NLQLEPDDLR KVIMQQARNF PGQENMIFDF YKNNPRAIEG LKGPALEDKA VQYIFNNEIK 430 440 LKEKKYTKEE LEKYLEAEEQ RITLI

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY62187.1.
RefSeq	YP_247352.1. NC_007109.1.
3D structure databases
ProteinModelPortal	Q4UJV2.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_1336.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY62187; AAY62187; RF_1336.
GeneID	3400892.
KEGG	rfe:RF_1336.
PATRIC	17893596. VBIRicFel64634_1608.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0544.
HOGENOM	HOG000218241.
KO	K03545.
OMA	FPADYHG.
ProtClustDB	PRK01490.
Enzyme and pathway databases
BioCyc	RFEL315456:GKEK-1373-MONOMER.
Family and domain databases
Gene3D	1.10.3120.10. 1 hit. 3.30.70.1050. 1 hit.
HAMAP	MF_00303. Trigger_factor_Tig.
InterPro	IPR001179. PPIase_FKBP_dom. IPR005215. Trig_fac. IPR008880. Trigger_fac_C. IPR008881. Trigger_fac_ribosome-bd_bac. IPR027304. Trigger_fact/SurA_dom. [Graphical view]
Pfam	PF00254. FKBP_C. 1 hit. PF05698. Trigger_C. 1 hit. PF05697. Trigger_N. 1 hit. [Graphical view]
PIRSF	PIRSF003095. Trigger_factor. 1 hit.
SUPFAM	SSF109998. Trigger_fac_C_bac. 1 hit. SSF102735. Trigger_fac_ribosome-bd_bac. 1 hit.
TIGRFAMs	TIGR00115. tig. 1 hit.
PROSITE	PS50059. FKBP_PPIASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TIG_RICFE

Accession

Primary (citable) accession number: Q4UJV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	July 5, 2005
Last modified:	May 29, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program