ID   HEMK_RICFE              Reviewed;         527 AA.
AC   Q4UJU4;
DT   04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   16-JUN-2009, entry version 30.
DE   RecName: Full=Bifunctional methyltransferase;
DE   Includes:
DE     RecName: Full=HemK protein homolog;
DE              EC=2.1.1.-;
DE   Includes:
DE     RecName: Full=tRNA (guanine-N(7)-)-methyltransferase;
DE              EC=2.1.1.33;
DE     AltName: Full=tRNA(m7G46)-methyltransferase;
GN   Name=hemK; OrderedLocusNames=RF_1344;
OS   Rickettsia felis (Rickettsia azadi).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX   NCBI_TaxID=42862;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC VR-1525 / URRWXCal2;
RX   PubMed=15984913; DOI=10.1371/journal.pbio.0030248;
RA   Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E.,
RA   Parinello H., Claverie J.-M., Raoult D.;
RT   "The genome sequence of Rickettsia felis identifies the first putative
RT   conjugative plasmid in an obligate intracellular parasite.";
RL   PLoS Biol. 3:1-12(2005).
CC   -!- FUNCTION: Catalyzes the formation of N(7)-methylguanine at
CC       position 46 (m7G46) in tRNA (By similarity).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-
CC       homocysteine + tRNA containing N(7)-methylguanine.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the hemK family.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the
CC       methyltransferase superfamily. TrmB family.
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DR   EMBL; CP000053; AAY62195.1; -; Genomic_DNA.
DR   RefSeq; YP_247360.1; -.
DR   GeneID; 3400900; -.
DR   GenomeReviews; CP000053_GR; RF_1344.
DR   KEGG; rfe:RF_1344; -.
DR   NMPDR; fig|315456.3.peg.1344; -.
DR   HOGENOM; Q4UJU4; -.
DR   OMA; Q4UJU4; IGVEVYL.
DR   BioCyc; RFEL315456:RF_1344-MON; -.
DR   BRENDA; 2.1.1.33; 297363.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0008276; F:protein methyltransferase activity; IEA:InterPro.
DR   GO; GO:0008176; F:tRNA (guanine-N7-)-methyltransferase activity; IEA:HAMAP.
DR   GO; GO:0006479; P:protein amino acid methylation; IEA:InterPro.
DR   GO; GO:0006400; P:tRNA modification; IEA:HAMAP.
DR   HAMAP; MF_01057; fused; 1.
DR   InterPro; IPR004556; Modification_methylase_HemK.
DR   InterPro; IPR002052; N6_adenine_Mtase_CS.
DR   InterPro; IPR019874; Release_fac_Glu-N5_MeTfrase.
DR   InterPro; IPR007848; Small_mtfrase.
DR   InterPro; IPR003358; tRNA_(Gua-N-7)_MeTrfase.
DR   Pfam; PF02390; Methyltransf_4; 1.
DR   Pfam; PF05175; MTS; 1.
DR   TIGRFAMs; TIGR00091; CHP91; 1.
DR   TIGRFAMs; TIGR00536; hemK_fam; 1.
DR   TIGRFAMs; TIGR03534; RF_mod_HemK; 1.
DR   PROSITE; PS00092; N6_MTASE; UNKNOWN_1.
PE   3: Inferred from homology;
KW   Complete proteome; Methyltransferase; S-adenosyl-L-methionine;
KW   Transferase; tRNA processing.
FT   CHAIN         1    527       Bifunctional methyltransferase.
FT                                /FTId=PRO_0000229298.
FT   REGION        1    309       HemK.
FT   REGION      310    527       tRNA (guanine-N(7)-)-methyltransferase.
FT   BINDING     356    356       S-adenosyl-L-methionine (By similarity).
FT   BINDING     381    381       S-adenosyl-L-methionine (By similarity).
FT   BINDING     408    408       S-adenosyl-L-methionine (By similarity).
FT   BINDING     430    430       S-adenosyl-L-methionine (By similarity).
FT   BINDING     434    434       Substrate (By similarity).
FT   BINDING     466    466       Substrate (Potential).
SQ   SEQUENCE   527 AA;  60400 MW;  33F7BD26BBCC46E3 CRC64;
     MQYSIKQVLS KASDKLNKIG ISSSQLEARI LLRYVINKPI EYLLINLDEQ LNEVEIEAFE
     KLLERRLKHE PIAYIIGIKE FYSREFIVNK HVLIPRADTE VLVDVCVHKS SLRATKRSVA
     ISGILSKIAS STPMASSRND EYTKILELGT GSGCIAISLL CELPNARVVA TDISLDAIEV
     ARNNALKYHV TDRIQIIHSN WFENLGKQKF DVIVSNPPYI STDEKPEMAL ETLNHEPYIA
     LFAEEDGLQA YRIIAENAKK FLKPNGKIVL EIGFKQEEAV TQIFLSNGYN IESVYKDLQG
     HSRVILFSPI NLNRSYARRI GKSLSGLQQN LLDNELPKYL FSKEKLIDEK RKIFLEIGFG
     MGEHFINQAK MNPDALFIGV EVYLNGVANV LKLASEQNIT NFLLFPNNLD FILNDLPNNS
     LDGIYILFPD PWIKNKQKKK RIFNKERLKI LQDKLKDNGN LVFASDIENY FYEAIELIEQ
     NSNFKIMNKN NYLKPHDNYV ITKYHQKAIK ANRIPRFIIL QHVSGDH
//