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Q4UJU4

- RFTRM_RICFE

UniProt

Q4UJU4 - RFTRM_RICFE

Protein

Bifunctional methyltransferase

Gene

prmC/trmB

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (05 Jul 2005)
      Previous versions | rss
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    Functioni

    Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.By similarity
    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine.
    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei172 – 1721S-adenosyl-L-methionineBy similarity
    Binding sitei201 – 2011S-adenosyl-L-methionineBy similarity
    Binding sitei216 – 2161S-adenosyl-L-methionineBy similarity
    Binding sitei356 – 3561S-adenosyl-L-methionineBy similarity
    Binding sitei381 – 3811S-adenosyl-L-methionineBy similarity
    Binding sitei408 – 4081S-adenosyl-L-methionineBy similarity
    Active sitei430 – 4301By similarity
    Binding sitei430 – 4301S-adenosyl-L-methionineBy similarity
    Binding sitei434 – 4341SubstrateBy similarity
    Binding sitei466 – 4661SubstrateBy similarity

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. protein methyltransferase activity Source: InterPro
    3. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciRFEL315456:GKEK-1381-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional methyltransferase
    Including the following 2 domains:
    Release factor glutamine methyltransferase (EC:2.1.1.297)
    Short name:
    RF MTase
    Alternative name(s):
    N5-glutamine methyltransferase PrmC
    Protein-(glutamine-N5) MTase PrmC
    Protein-glutamine N-methyltransferase PrmC
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    Name:prmC/trmB
    Synonyms:hemK
    Ordered Locus Names:RF_1344
    OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
    Taxonomic identifieri315456 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
    ProteomesiUP000008548: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 527527Bifunctional methyltransferasePRO_0000229298Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi315456.RF_1344.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4UJU4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 311311RF MTaseAdd
    BLAST
    Regioni1 – 309309HemKAdd
    BLAST
    Regioni149 – 1535S-adenosyl-L-methionine bindingBy similarity
    Regioni216 – 2194Substrate bindingBy similarity
    Regioni310 – 527218tRNA (guanine-N(7)-)-methyltransferaseAdd
    BLAST
    Regioni314 – 527214tRNA MTaseAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.Curated
    In the N-terminal section; belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000010896.
    KOiK02493.
    OMAiQQNLLDN.
    OrthoDBiEOG68Q0SZ.

    Family and domain databases

    Gene3Di3.40.50.150. 2 hits.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    MF_02126. RF_methyltr_PrmC.
    InterProiIPR002052. DNA_methylase_N6_adenine_CS.
    IPR004556. Modification_methylase_HemK.
    IPR019874. Release_fac_Glu-N5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    IPR007848. Small_mtfrase_dom.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    PF05175. MTS. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 3 hits.
    TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
    TIGR03534. RF_mod_PrmC. 1 hit.
    TIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4UJU4-1 [UniParc]FASTAAdd to Basket

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    MQYSIKQVLS KASDKLNKIG ISSSQLEARI LLRYVINKPI EYLLINLDEQ    50
    LNEVEIEAFE KLLERRLKHE PIAYIIGIKE FYSREFIVNK HVLIPRADTE 100
    VLVDVCVHKS SLRATKRSVA ISGILSKIAS STPMASSRND EYTKILELGT 150
    GSGCIAISLL CELPNARVVA TDISLDAIEV ARNNALKYHV TDRIQIIHSN 200
    WFENLGKQKF DVIVSNPPYI STDEKPEMAL ETLNHEPYIA LFAEEDGLQA 250
    YRIIAENAKK FLKPNGKIVL EIGFKQEEAV TQIFLSNGYN IESVYKDLQG 300
    HSRVILFSPI NLNRSYARRI GKSLSGLQQN LLDNELPKYL FSKEKLIDEK 350
    RKIFLEIGFG MGEHFINQAK MNPDALFIGV EVYLNGVANV LKLASEQNIT 400
    NFLLFPNNLD FILNDLPNNS LDGIYILFPD PWIKNKQKKK RIFNKERLKI 450
    LQDKLKDNGN LVFASDIENY FYEAIELIEQ NSNFKIMNKN NYLKPHDNYV 500
    ITKYHQKAIK ANRIPRFIIL QHVSGDH 527
    Length:527
    Mass (Da):60,400
    Last modified:July 5, 2005 - v1
    Checksum:i33F7BD26BBCC46E3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000053 Genomic DNA. Translation: AAY62195.1.
    RefSeqiYP_247360.1. NC_007109.1.

    Genome annotation databases

    EnsemblBacteriaiAAY62195; AAY62195; RF_1344.
    GeneIDi3400900.
    KEGGirfe:RF_1344.
    PATRICi17893616. VBIRicFel64634_1618.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000053 Genomic DNA. Translation: AAY62195.1 .
    RefSeqi YP_247360.1. NC_007109.1.

    3D structure databases

    ProteinModelPortali Q4UJU4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 315456.RF_1344.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY62195 ; AAY62195 ; RF_1344 .
    GeneIDi 3400900.
    KEGGi rfe:RF_1344.
    PATRICi 17893616. VBIRicFel64634_1618.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000010896.
    KOi K02493.
    OMAi QQNLLDN.
    OrthoDBi EOG68Q0SZ.

    Enzyme and pathway databases

    BioCyci RFEL315456:GKEK-1381-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 2 hits.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    MF_02126. RF_methyltr_PrmC.
    InterProi IPR002052. DNA_methylase_N6_adenine_CS.
    IPR004556. Modification_methylase_HemK.
    IPR019874. Release_fac_Glu-N5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    IPR007848. Small_mtfrase_dom.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    PF05175. MTS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 3 hits.
    TIGRFAMsi TIGR00536. hemK_fam. 1 hit.
    TIGR03534. RF_mod_PrmC. 1 hit.
    TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
      Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
      PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC VR-1525 / URRWXCal2.

    Entry informationi

    Entry nameiRFTRM_RICFE
    AccessioniPrimary (citable) accession number: Q4UJU4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: July 5, 2005
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Rickettsia felis (Rickettsia azadi)
      Rickettsia felis (strain URRWXCal2): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3