Reviewed, UniProtKB/Swiss-Prot Q4UJU4 (HEMK_RICFE)

Names and origin

Protein names

Recommended name:
    Bifunctional methyltransferase
Including the following 2 domains:
    1- Recommended name:
            HemK protein homolog
              EC=2.1.1.-
    2- Recommended name:
            tRNA (guanine-N(7)-)-methyltransferase
              EC=2.1.1.33
        Alternative name(s):
            tRNA(m7G46)-methyltransferase

Gene names

Name:	hemK
Ordered Locus Names:	RF_1344

Organism

Rickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

42862 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(7)-methylguanine. HAMAP MF_01057
Sequence similarities	In the N-terminal section; belongs to the hemK family. In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid methylation Inferred from electronic annotation. Source: InterPro tRNA modification Inferred from electronic annotation. Source: HAMAP
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro protein methyltransferase activity Inferred from electronic annotation. Source: InterPro tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 527	527	Bifunctional methyltransferase HAMAP MF_01057		PRO_0000229298
Regions
	Region	1 – 309	309	HemK HAMAP MF_01057
	Region	310 – 527	218	tRNA (guanine-N(7)-)-methyltransferase HAMAP MF_01057
Sites
	Binding site	356	1	S-adenosyl-L-methionine By similarity
	Binding site	381	1	S-adenosyl-L-methionine By similarity
	Binding site	408	1	S-adenosyl-L-methionine By similarity
	Binding site	430	1	S-adenosyl-L-methionine By similarity
	Binding site	434	1	Substrate By similarity
	Binding site	466	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4UJU4-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 33F7BD26BBCC46E3	FASTA	527	60,400
	10 20 30 40 50 60 MQYSIKQVLS KASDKLNKIG ISSSQLEARI LLRYVINKPI EYLLINLDEQ LNEVEIEAFE 70 80 90 100 110 120 KLLERRLKHE PIAYIIGIKE FYSREFIVNK HVLIPRADTE VLVDVCVHKS SLRATKRSVA 130 140 150 160 170 180 ISGILSKIAS STPMASSRND EYTKILELGT GSGCIAISLL CELPNARVVA TDISLDAIEV 190 200 210 220 230 240 ARNNALKYHV TDRIQIIHSN WFENLGKQKF DVIVSNPPYI STDEKPEMAL ETLNHEPYIA 250 260 270 280 290 300 LFAEEDGLQA YRIIAENAKK FLKPNGKIVL EIGFKQEEAV TQIFLSNGYN IESVYKDLQG 310 320 330 340 350 360 HSRVILFSPI NLNRSYARRI GKSLSGLQQN LLDNELPKYL FSKEKLIDEK RKIFLEIGFG 370 380 390 400 410 420 MGEHFINQAK MNPDALFIGV EVYLNGVANV LKLASEQNIT NFLLFPNNLD FILNDLPNNS 430 440 450 460 470 480 LDGIYILFPD PWIKNKQKKK RIFNKERLKI LQDKLKDNGN LVFASDIENY FYEAIELIEQ 490 500 510 520 NSNFKIMNKN NYLKPHDNYV ITKYHQKAIK ANRIPRFIIL QHVSGDH

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000053 Genomic DNA. Translation: AAY62195.1.
RefSeq	YP_247360.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3400900.
GenomeReviews	Gene locus RF_1344 in contig CP000053_GR.
KEGG	rfe:RF_1344.
NMPDR	fig|315456.3.peg.1344.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4UJU4.
OMA	Q4UJU4. IGVEVYL.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_1344-MON.
BRENDA	2.1.1.33. 297363.
Family and domain databases
HAMAP	MF_01057. Fused. [Tree]
InterPro	IPR004556. Modification_methylase_HemK. IPR002052. N6_adenine_Mtase_CS. IPR019874. Release_fac_Glu-N5_MeTfrase. IPR007848. Small_mtfrase. IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. PF05175. MTS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. CHP91. 1 hit. TIGR00536. hemK_fam. 1 hit. TIGR03534. RF_mod_HemK. 1 hit.
PROSITE	PS00092. N6_MTASE. 1 hit. Uncertain. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMK_RICFE

Accession

Primary (citable) accession number: Q4UJU4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	July 5, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)