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Reviewed, UniProtKB/Swiss-Prot Q4UJU4 (HEMK_RICFE)

Last modified June 16, 2009. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Bifunctional methyltransferase
Including the following 2 domains:
    1- Recommended name:
            HemK protein homolog
              EC=2.1.1.-
    2- Recommended name:
            tRNA (guanine-N(7)-)-methyltransferase
              EC=2.1.1.33
        Alternative name(s):
            tRNA(m7G46)-methyltransferase
Gene names
Name: hemK
Ordered Locus Names: RF_1344
OrganismRickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier42862 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length527 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.

Catalytic activity

S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(7)-methylguanine. HAMAP MF_01057

Sequence similarities

In the N-terminal section; belongs to the hemK family.

In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 527527Bifunctional methyltransferase HAMAP MF_01057
PRO_0000229298

Regions

Region1 – 309309HemK HAMAP MF_01057
Region310 – 527218tRNA (guanine-N(7)-)-methyltransferase HAMAP MF_01057

Sites

Binding site3561S-adenosyl-L-methionine By similarity
Binding site3811S-adenosyl-L-methionine By similarity
Binding site4081S-adenosyl-L-methionine By similarity
Binding site4301S-adenosyl-L-methionine By similarity
Binding site4341Substrate By similarity
Binding site4661Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
Q4UJU4-1 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: 33F7BD26BBCC46E3

FASTA52760,400
        10         20         30         40         50         60 
MQYSIKQVLS KASDKLNKIG ISSSQLEARI LLRYVINKPI EYLLINLDEQ LNEVEIEAFE 

        70         80         90        100        110        120 
KLLERRLKHE PIAYIIGIKE FYSREFIVNK HVLIPRADTE VLVDVCVHKS SLRATKRSVA 

       130        140        150        160        170        180 
ISGILSKIAS STPMASSRND EYTKILELGT GSGCIAISLL CELPNARVVA TDISLDAIEV 

       190        200        210        220        230        240 
ARNNALKYHV TDRIQIIHSN WFENLGKQKF DVIVSNPPYI STDEKPEMAL ETLNHEPYIA 

       250        260        270        280        290        300 
LFAEEDGLQA YRIIAENAKK FLKPNGKIVL EIGFKQEEAV TQIFLSNGYN IESVYKDLQG 

       310        320        330        340        350        360 
HSRVILFSPI NLNRSYARRI GKSLSGLQQN LLDNELPKYL FSKEKLIDEK RKIFLEIGFG 

       370        380        390        400        410        420 
MGEHFINQAK MNPDALFIGV EVYLNGVANV LKLASEQNIT NFLLFPNNLD FILNDLPNNS 

       430        440        450        460        470        480 
LDGIYILFPD PWIKNKQKKK RIFNKERLKI LQDKLKDNGN LVFASDIENY FYEAIELIEQ 

       490        500        510        520 
NSNFKIMNKN NYLKPHDNYV ITKYHQKAIK ANRIPRFIIL QHVSGDH 

« Hide

References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

CP000053 Genomic DNA. Translation: AAY62195.1.
RefSeqYP_247360.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID3400900.
GenomeReviewsGene locus RF_1344 in contig CP000053_GR.
KEGGrfe:RF_1344.
NMPDRfig|315456.3.peg.1344.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ4UJU4.
OMAQ4UJU4. IGVEVYL.

Enzyme and pathway databases

BioCycRFEL315456:RF_1344-MON.
BRENDA2.1.1.33. 297363.

Family and domain databases

HAMAPMF_01057. Fused.
[Tree]
InterProIPR004556. Modification_methylase_HemK.
IPR002052. N6_adenine_Mtase_CS.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR007848. Small_mtfrase.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. CHP91. 1 hit.
TIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_HemK. 1 hit.
PROSITEPS00092. N6_MTASE. 1 hit. Uncertain.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEMK_RICFE
AccessionPrimary (citable) accession number: Q4UJU4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: July 5, 2005
Last modified: June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents