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Q4UJU4

- RFTRM_RICFE

UniProt

Q4UJU4 - RFTRM_RICFE

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Protein
Bifunctional methyltransferase
Gene
prmC/trmB, hemK, RF_1344
Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif By similarity.UniRule annotation
Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine.UniRule annotation
S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei172 – 1721S-adenosyl-L-methionine By similarity
Binding sitei201 – 2011S-adenosyl-L-methionine By similarity
Binding sitei216 – 2161S-adenosyl-L-methionine By similarity
Binding sitei356 – 3561S-adenosyl-L-methionine By similarity
Binding sitei381 – 3811S-adenosyl-L-methionine By similarity
Binding sitei408 – 4081S-adenosyl-L-methionine By similarity
Active sitei430 – 4301 By similarity
Binding sitei430 – 4301S-adenosyl-L-methionine By similarity
Binding sitei434 – 4341Substrate By similarity
Binding sitei466 – 4661Substrate By similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. protein methyltransferase activity Source: InterPro
  3. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-1381-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional methyltransferase
Including the following 2 domains:
Release factor glutamine methyltransferase (EC:2.1.1.297)
Short name:
RF MTase
Alternative name(s):
N5-glutamine methyltransferase PrmC
Protein-(glutamine-N5) MTase PrmC
Protein-glutamine N-methyltransferase PrmC
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:prmC/trmB
Synonyms:hemK
Ordered Locus Names:RF_1344
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000008548: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 527527Bifunctional methyltransferaseUniRule annotation
PRO_0000229298Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi315456.RF_1344.

Structurei

3D structure databases

ProteinModelPortaliQ4UJU4.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 311311RF MTaseUniRule annotation
Add
BLAST
Regioni1 – 309309HemKUniRule annotation
Add
BLAST
Regioni149 – 1535S-adenosyl-L-methionine binding By similarity
Regioni216 – 2194Substrate binding By similarity
Regioni310 – 527218tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
Add
BLAST
Regioni314 – 527214tRNA MTaseUniRule annotation
Add
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.
In the N-terminal section; belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000010896.
KOiK02493.
OMAiQQNLLDN.
OrthoDBiEOG68Q0SZ.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
MF_02126. RF_methyltr_PrmC.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 3 hits.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4UJU4-1 [UniParc]FASTAAdd to Basket

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MQYSIKQVLS KASDKLNKIG ISSSQLEARI LLRYVINKPI EYLLINLDEQ    50
LNEVEIEAFE KLLERRLKHE PIAYIIGIKE FYSREFIVNK HVLIPRADTE 100
VLVDVCVHKS SLRATKRSVA ISGILSKIAS STPMASSRND EYTKILELGT 150
GSGCIAISLL CELPNARVVA TDISLDAIEV ARNNALKYHV TDRIQIIHSN 200
WFENLGKQKF DVIVSNPPYI STDEKPEMAL ETLNHEPYIA LFAEEDGLQA 250
YRIIAENAKK FLKPNGKIVL EIGFKQEEAV TQIFLSNGYN IESVYKDLQG 300
HSRVILFSPI NLNRSYARRI GKSLSGLQQN LLDNELPKYL FSKEKLIDEK 350
RKIFLEIGFG MGEHFINQAK MNPDALFIGV EVYLNGVANV LKLASEQNIT 400
NFLLFPNNLD FILNDLPNNS LDGIYILFPD PWIKNKQKKK RIFNKERLKI 450
LQDKLKDNGN LVFASDIENY FYEAIELIEQ NSNFKIMNKN NYLKPHDNYV 500
ITKYHQKAIK ANRIPRFIIL QHVSGDH 527
Length:527
Mass (Da):60,400
Last modified:July 5, 2005 - v1
Checksum:i33F7BD26BBCC46E3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000053 Genomic DNA. Translation: AAY62195.1.
RefSeqiYP_247360.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY62195; AAY62195; RF_1344.
GeneIDi3400900.
KEGGirfe:RF_1344.
PATRICi17893616. VBIRicFel64634_1618.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000053 Genomic DNA. Translation: AAY62195.1 .
RefSeqi YP_247360.1. NC_007109.1.

3D structure databases

ProteinModelPortali Q4UJU4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 315456.RF_1344.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY62195 ; AAY62195 ; RF_1344 .
GeneIDi 3400900.
KEGGi rfe:RF_1344.
PATRICi 17893616. VBIRicFel64634_1618.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000010896.
KOi K02493.
OMAi QQNLLDN.
OrthoDBi EOG68Q0SZ.

Enzyme and pathway databases

BioCyci RFEL315456:GKEK-1381-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
MF_02126. RF_methyltr_PrmC.
InterProi IPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 3 hits.
TIGRFAMsi TIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.

Entry informationi

Entry nameiRFTRM_RICFE
AccessioniPrimary (citable) accession number: Q4UJU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: July 5, 2005
Last modified: June 11, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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