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Protein

Bifunctional methyltransferase

Gene

prmC/trmB

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.By similarity
Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.By similarity

Catalytic activityi

S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine.
S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei172S-adenosyl-L-methionineBy similarity1
Binding sitei201S-adenosyl-L-methionineBy similarity1
Binding sitei216S-adenosyl-L-methionineBy similarity1
Binding sitei356S-adenosyl-L-methionineBy similarity1
Binding sitei381S-adenosyl-L-methionineBy similarity1
Binding sitei408S-adenosyl-L-methionineBy similarity1
Active sitei430By similarity1
Binding sitei430S-adenosyl-L-methionineBy similarity1
Binding sitei434SubstrateBy similarity1
Binding sitei466SubstrateBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional methyltransferase
Including the following 2 domains:
Release factor glutamine methyltransferase (EC:2.1.1.297)
Short name:
RF MTase
Alternative name(s):
N5-glutamine methyltransferase PrmC
Protein-(glutamine-N5) MTase PrmC
Protein-glutamine N-methyltransferase PrmC
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:prmC/trmB
Synonyms:hemK
Ordered Locus Names:RF_1344
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
Proteomesi
  • UP000008548 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002292981 – 527Bifunctional methyltransferaseAdd BLAST527

Interactioni

Protein-protein interaction databases

STRINGi315456.RF_1344.

Structurei

3D structure databases

ProteinModelPortaliQ4UJU4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 311RF MTaseAdd BLAST311
Regioni1 – 309HemKAdd BLAST309
Regioni149 – 153S-adenosyl-L-methionine bindingBy similarity5
Regioni216 – 219Substrate bindingBy similarity4
Regioni310 – 527tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST218
Regioni314 – 527tRNA MTaseAdd BLAST214

Sequence similaritiesi

In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.Curated
In the N-terminal section; belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105EQY. Bacteria.
COG0220. LUCA.
COG2890. LUCA.
HOGENOMiHOG000010896.
KOiK02493.
OMAiDDISENC.
OrthoDBiPOG091H02CJ.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
HAMAPiMF_02126. RF_methyltr_PrmC. 1 hit.
MF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 3 hits.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4UJU4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQYSIKQVLS KASDKLNKIG ISSSQLEARI LLRYVINKPI EYLLINLDEQ
60 70 80 90 100
LNEVEIEAFE KLLERRLKHE PIAYIIGIKE FYSREFIVNK HVLIPRADTE
110 120 130 140 150
VLVDVCVHKS SLRATKRSVA ISGILSKIAS STPMASSRND EYTKILELGT
160 170 180 190 200
GSGCIAISLL CELPNARVVA TDISLDAIEV ARNNALKYHV TDRIQIIHSN
210 220 230 240 250
WFENLGKQKF DVIVSNPPYI STDEKPEMAL ETLNHEPYIA LFAEEDGLQA
260 270 280 290 300
YRIIAENAKK FLKPNGKIVL EIGFKQEEAV TQIFLSNGYN IESVYKDLQG
310 320 330 340 350
HSRVILFSPI NLNRSYARRI GKSLSGLQQN LLDNELPKYL FSKEKLIDEK
360 370 380 390 400
RKIFLEIGFG MGEHFINQAK MNPDALFIGV EVYLNGVANV LKLASEQNIT
410 420 430 440 450
NFLLFPNNLD FILNDLPNNS LDGIYILFPD PWIKNKQKKK RIFNKERLKI
460 470 480 490 500
LQDKLKDNGN LVFASDIENY FYEAIELIEQ NSNFKIMNKN NYLKPHDNYV
510 520
ITKYHQKAIK ANRIPRFIIL QHVSGDH
Length:527
Mass (Da):60,400
Last modified:July 5, 2005 - v1
Checksum:i33F7BD26BBCC46E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY62195.1.
RefSeqiWP_011271644.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY62195; AAY62195; RF_1344.
KEGGirfe:RF_1344.
PATRICi17893616. VBIRicFel64634_1618.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY62195.1.
RefSeqiWP_011271644.1. NC_007109.1.

3D structure databases

ProteinModelPortaliQ4UJU4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi315456.RF_1344.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY62195; AAY62195; RF_1344.
KEGGirfe:RF_1344.
PATRICi17893616. VBIRicFel64634_1618.

Phylogenomic databases

eggNOGiENOG4105EQY. Bacteria.
COG0220. LUCA.
COG2890. LUCA.
HOGENOMiHOG000010896.
KOiK02493.
OMAiDDISENC.
OrthoDBiPOG091H02CJ.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
HAMAPiMF_02126. RF_methyltr_PrmC. 1 hit.
MF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 3 hits.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRFTRM_RICFE
AccessioniPrimary (citable) accession number: Q4UJU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: July 5, 2005
Last modified: November 2, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.