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Q4UJU4 (RFTRM_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional methyltransferase

Including the following 2 domains:

  1. Release factor glutamine methyltransferase
    Short name=RF MTase
    EC=2.1.1.297
    Alternative name(s):
    N5-glutamine methyltransferase PrmC
    Protein-(glutamine-N5) MTase PrmC
    Protein-glutamine N-methyltransferase PrmC
  2. tRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.33
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
Gene names
Name:prmC/trmB
Synonyms:hemK
Ordered Locus Names:RF_1344
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length527 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif By similarity. HAMAP-Rule MF_01057

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine. HAMAP-Rule MF_01057

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Sequence similarities

In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

In the N-terminal section; belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 527527Bifunctional methyltransferase HAMAP-Rule MF_01057
PRO_0000229298

Regions

Region1 – 311311RF MTase HAMAP-Rule MF_01057
Region1 – 309309HemK HAMAP-Rule MF_01057
Region149 – 1535S-adenosyl-L-methionine binding By similarity
Region216 – 2194Substrate binding By similarity
Region310 – 527218tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
Region314 – 527214tRNA MTase HAMAP-Rule MF_01057

Sites

Active site4301 By similarity
Binding site1721S-adenosyl-L-methionine By similarity
Binding site2011S-adenosyl-L-methionine By similarity
Binding site2161S-adenosyl-L-methionine By similarity
Binding site3561S-adenosyl-L-methionine By similarity
Binding site3811S-adenosyl-L-methionine By similarity
Binding site4081S-adenosyl-L-methionine By similarity
Binding site4301S-adenosyl-L-methionine By similarity
Binding site4341Substrate By similarity
Binding site4661Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4UJU4 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: 33F7BD26BBCC46E3

FASTA52760,400
        10         20         30         40         50         60 
MQYSIKQVLS KASDKLNKIG ISSSQLEARI LLRYVINKPI EYLLINLDEQ LNEVEIEAFE 

        70         80         90        100        110        120 
KLLERRLKHE PIAYIIGIKE FYSREFIVNK HVLIPRADTE VLVDVCVHKS SLRATKRSVA 

       130        140        150        160        170        180 
ISGILSKIAS STPMASSRND EYTKILELGT GSGCIAISLL CELPNARVVA TDISLDAIEV 

       190        200        210        220        230        240 
ARNNALKYHV TDRIQIIHSN WFENLGKQKF DVIVSNPPYI STDEKPEMAL ETLNHEPYIA 

       250        260        270        280        290        300 
LFAEEDGLQA YRIIAENAKK FLKPNGKIVL EIGFKQEEAV TQIFLSNGYN IESVYKDLQG 

       310        320        330        340        350        360 
HSRVILFSPI NLNRSYARRI GKSLSGLQQN LLDNELPKYL FSKEKLIDEK RKIFLEIGFG 

       370        380        390        400        410        420 
MGEHFINQAK MNPDALFIGV EVYLNGVANV LKLASEQNIT NFLLFPNNLD FILNDLPNNS 

       430        440        450        460        470        480 
LDGIYILFPD PWIKNKQKKK RIFNKERLKI LQDKLKDNGN LVFASDIENY FYEAIELIEQ 

       490        500        510        520 
NSNFKIMNKN NYLKPHDNYV ITKYHQKAIK ANRIPRFIIL QHVSGDH 

« Hide

References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000053 Genomic DNA. Translation: AAY62195.1.
RefSeqYP_247360.1. NC_007109.1.

3D structure databases

ProteinModelPortalQ4UJU4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING315456.RF_1344.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY62195; AAY62195; RF_1344.
GeneID3400900.
KEGGrfe:RF_1344.
PATRIC17893616. VBIRicFel64634_1618.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000010896.
KOK02493.
OMAQQNLLDN.
OrthoDBEOG68Q0SZ.

Enzyme and pathway databases

BioCycRFEL315456:GKEK-1381-MONOMER.

Family and domain databases

Gene3D3.40.50.150. 2 hits.
HAMAPMF_01057. tRNA_methyltr_TrmB.
MF_02126. RF_methyltr_PrmC.
InterProIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 3 hits.
TIGRFAMsTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRFTRM_RICFE
AccessionPrimary (citable) accession number: Q4UJU4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: July 5, 2005
Last modified: June 11, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names