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Q4UJU4

- RFTRM_RICFE

UniProt

Q4UJU4 - RFTRM_RICFE

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Protein

Bifunctional methyltransferase

Gene

prmC/trmB

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.By similarity
Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.By similarity

Catalytic activityi

S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine.
S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei172 – 1721S-adenosyl-L-methionineBy similarity
Binding sitei201 – 2011S-adenosyl-L-methionineBy similarity
Binding sitei216 – 2161S-adenosyl-L-methionineBy similarity
Binding sitei356 – 3561S-adenosyl-L-methionineBy similarity
Binding sitei381 – 3811S-adenosyl-L-methionineBy similarity
Binding sitei408 – 4081S-adenosyl-L-methionineBy similarity
Active sitei430 – 4301By similarity
Binding sitei430 – 4301S-adenosyl-L-methionineBy similarity
Binding sitei434 – 4341SubstrateBy similarity
Binding sitei466 – 4661SubstrateBy similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. protein methyltransferase activity Source: InterPro
  3. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-1381-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional methyltransferase
Including the following 2 domains:
Release factor glutamine methyltransferase (EC:2.1.1.297)
Short name:
RF MTase
Alternative name(s):
N5-glutamine methyltransferase PrmC
Protein-(glutamine-N5) MTase PrmC
Protein-glutamine N-methyltransferase PrmC
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:prmC/trmB
Synonyms:hemK
Ordered Locus Names:RF_1344
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000008548: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 527527Bifunctional methyltransferasePRO_0000229298Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi315456.RF_1344.

Structurei

3D structure databases

ProteinModelPortaliQ4UJU4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 311311RF MTaseAdd
BLAST
Regioni1 – 309309HemKAdd
BLAST
Regioni149 – 1535S-adenosyl-L-methionine bindingBy similarity
Regioni216 – 2194Substrate bindingBy similarity
Regioni310 – 527218tRNA (guanine-N(7)-)-methyltransferaseAdd
BLAST
Regioni314 – 527214tRNA MTaseAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.Curated
In the N-terminal section; belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.Curated

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000010896.
KOiK02493.
OMAiQQNLLDN.
OrthoDBiEOG68Q0SZ.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
MF_02126. RF_methyltr_PrmC.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 3 hits.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4UJU4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQYSIKQVLS KASDKLNKIG ISSSQLEARI LLRYVINKPI EYLLINLDEQ
60 70 80 90 100
LNEVEIEAFE KLLERRLKHE PIAYIIGIKE FYSREFIVNK HVLIPRADTE
110 120 130 140 150
VLVDVCVHKS SLRATKRSVA ISGILSKIAS STPMASSRND EYTKILELGT
160 170 180 190 200
GSGCIAISLL CELPNARVVA TDISLDAIEV ARNNALKYHV TDRIQIIHSN
210 220 230 240 250
WFENLGKQKF DVIVSNPPYI STDEKPEMAL ETLNHEPYIA LFAEEDGLQA
260 270 280 290 300
YRIIAENAKK FLKPNGKIVL EIGFKQEEAV TQIFLSNGYN IESVYKDLQG
310 320 330 340 350
HSRVILFSPI NLNRSYARRI GKSLSGLQQN LLDNELPKYL FSKEKLIDEK
360 370 380 390 400
RKIFLEIGFG MGEHFINQAK MNPDALFIGV EVYLNGVANV LKLASEQNIT
410 420 430 440 450
NFLLFPNNLD FILNDLPNNS LDGIYILFPD PWIKNKQKKK RIFNKERLKI
460 470 480 490 500
LQDKLKDNGN LVFASDIENY FYEAIELIEQ NSNFKIMNKN NYLKPHDNYV
510 520
ITKYHQKAIK ANRIPRFIIL QHVSGDH
Length:527
Mass (Da):60,400
Last modified:July 5, 2005 - v1
Checksum:i33F7BD26BBCC46E3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000053 Genomic DNA. Translation: AAY62195.1.
RefSeqiWP_011271644.1. NC_007109.1.
YP_247360.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY62195; AAY62195; RF_1344.
GeneIDi3400900.
KEGGirfe:RF_1344.
PATRICi17893616. VBIRicFel64634_1618.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000053 Genomic DNA. Translation: AAY62195.1 .
RefSeqi WP_011271644.1. NC_007109.1.
YP_247360.1. NC_007109.1.

3D structure databases

ProteinModelPortali Q4UJU4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 315456.RF_1344.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY62195 ; AAY62195 ; RF_1344 .
GeneIDi 3400900.
KEGGi rfe:RF_1344.
PATRICi 17893616. VBIRicFel64634_1618.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000010896.
KOi K02493.
OMAi QQNLLDN.
OrthoDBi EOG68Q0SZ.

Enzyme and pathway databases

BioCyci RFEL315456:GKEK-1381-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
MF_02126. RF_methyltr_PrmC.
InterProi IPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 3 hits.
TIGRFAMsi TIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.

Entry informationi

Entry nameiRFTRM_RICFE
AccessioniPrimary (citable) accession number: Q4UJU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: July 5, 2005
Last modified: October 29, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3