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Q4UJT5

- SYA_RICFE

UniProt

Q4UJT5 - SYA_RICFE

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Protein

Alanine--tRNA ligase

Gene
alaS, RF_1353
Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity.UniRule annotation

Catalytic activityi

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi567 – 5671Zinc Reviewed prediction
Metal bindingi571 – 5711Zinc Reviewed prediction
Metal bindingi669 – 6691Zinc Reviewed prediction
Metal bindingi673 – 6731Zinc Reviewed prediction

GO - Molecular functioni

  1. alanine-tRNA ligase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP
  3. tRNA binding Source: UniProtKB-KW
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. alanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-1390-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine--tRNA ligase (EC:6.1.1.7)
Alternative name(s):
Alanyl-tRNA synthetase
Short name:
AlaRS
Gene namesi
Name:alaS
Ordered Locus Names:RF_1353
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000008548: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 878878Alanine--tRNA ligaseUniRule annotationPRO_0000075191Add
BLAST

Proteomic databases

PRIDEiQ4UJT5.

Interactioni

Protein-protein interaction databases

STRINGi315456.RF_1353.

Structurei

3D structure databases

ProteinModelPortaliQ4UJT5.

Family & Domainsi

Domaini

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0013.
HOGENOMiHOG000156965.
KOiK01872.
OMAiYDIDIFQ.
OrthoDBiEOG6Q2SQ2.

Family and domain databases

HAMAPiMF_00036_B. Ala_tRNA_synth_B.
InterProiIPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR003156. DHHA1_dom.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR00980. TRNASYNTHALA.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsiTIGR00344. alaS. 1 hit.
PROSITEiPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4UJT5-1 [UniParc]FASTAAdd to Basket

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MTKFTTEEVR SKFITYFKAN NHTHVPASSL IPHNDPSLMF VNSGMVQFKN    50
VFTGQEKRPY NKAVTSQKSL RAGGKHNDLE NVGYTARHHT FFEMLGNFSF 100
GDYFKEQAIY YAWNLLTKEF ELPKDKLYVT VYHTDEEAAS YWKKIAGFRD 150
DRIIRIKTND NFWSMGDTGP CGPCSEIFYD HGEQIYGGLP GTKDEDGDRF 200
IEIWNMVFMQ YEQIDKDTRI ELPQKSIDTG MGLERMTAVL QHVNNNYDID 250
LFQEIINFTE NIVKVKIEGE AKFSYRVIAD HLRASSFLIA DGVIPSNEGR 300
GYVLRRIMRR AMRHAHMLGS KEPLMYKLLP KLVDLMGNVY PELKRAESFI 350
SSILEQEEIR FKTTLERGLK LLTEETETLT KGNELSGEIA FKLYDTYGFP 400
LDLTEDILKN RDISVDHKRF EEQMLAQKER ARKSWLGSGE SKTDQLWFDI 450
KEQHGSTEFL GYTLNEAECK IIALIKDNNL VNDIKEIDTQ FLLISNQTPF 500
YGESGGQMGD IGMIFSKDSE IEVIDTLKYL GSIIVHKCIL KKGQIKIGEN 550
ANFSIDIKYR QNLRIHHSAT HILHAVLHEV LGKHVTQKGS LVAPTYLRFD 600
ISHSKAVTNE EITLIEDKVN EIIRDNHEVD TTLMTTEDAV KQGAMALFGE 650
KYDSEVRVVK MGETSLELCG GTHVRRTGDI GCFKITSESA IAAGVRRIEA 700
VCGEFVIKLM REKDSLLKSI ESSLKTNKNE LITKVNNILE RNKELEKELE 750
KVHLARLDLS IEQIEKQAED IKGVKLIYRY IENLDNKVLR QAAENLTKKV 800
EDLIVVYITG NNDKLSITVA VSKAITDKYK AGIIAKELSL FLGGSGGGGQ 850
ASLAQAGGND IGKLTNIQEK LHGLLTVS 878
Length:878
Mass (Da):99,621
Last modified:July 5, 2005 - v1
Checksum:iCA941B9A40D10B98
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000053 Genomic DNA. Translation: AAY62204.1.
RefSeqiYP_247369.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY62204; AAY62204; RF_1353.
GeneIDi3400909.
KEGGirfe:RF_1353.
PATRICi17893638. VBIRicFel64634_1629.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000053 Genomic DNA. Translation: AAY62204.1 .
RefSeqi YP_247369.1. NC_007109.1.

3D structure databases

ProteinModelPortali Q4UJT5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 315456.RF_1353.

Proteomic databases

PRIDEi Q4UJT5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY62204 ; AAY62204 ; RF_1353 .
GeneIDi 3400909.
KEGGi rfe:RF_1353.
PATRICi 17893638. VBIRicFel64634_1629.

Phylogenomic databases

eggNOGi COG0013.
HOGENOMi HOG000156965.
KOi K01872.
OMAi YDIDIFQ.
OrthoDBi EOG6Q2SQ2.

Enzyme and pathway databases

BioCyci RFEL315456:GKEK-1390-MONOMER.

Family and domain databases

HAMAPi MF_00036_B. Ala_tRNA_synth_B.
InterProi IPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR003156. DHHA1_dom.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view ]
Pfami PF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view ]
PRINTSi PR00980. TRNASYNTHALA.
SMARTi SM00863. tRNA_SAD. 1 hit.
[Graphical view ]
SUPFAMi SSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsi TIGR00344. alaS. 1 hit.
PROSITEi PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.

Entry informationi

Entry nameiSYA_RICFE
AccessioniPrimary (citable) accession number: Q4UJT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: July 5, 2005
Last modified: September 3, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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