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Q4UJT5

- SYA_RICFE

UniProt

Q4UJT5 - SYA_RICFE

Protein

Alanine--tRNA ligase

Gene

alaS

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (05 Jul 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.UniRule annotation

    Catalytic activityi

    ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi567 – 5671ZincUniRule annotation
    Metal bindingi571 – 5711ZincUniRule annotation
    Metal bindingi669 – 6691ZincUniRule annotation
    Metal bindingi673 – 6731ZincUniRule annotation

    GO - Molecular functioni

    1. alanine-tRNA ligase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-HAMAP
    3. tRNA binding Source: UniProtKB-KW
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. alanyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

    Enzyme and pathway databases

    BioCyciRFEL315456:GKEK-1390-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alanine--tRNA ligaseUniRule annotation (EC:6.1.1.7UniRule annotation)
    Alternative name(s):
    Alanyl-tRNA synthetaseUniRule annotation
    Short name:
    AlaRSUniRule annotation
    Gene namesi
    Name:alaSUniRule annotation
    Ordered Locus Names:RF_1353
    OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
    Taxonomic identifieri315456 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
    ProteomesiUP000008548: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 878878Alanine--tRNA ligasePRO_0000075191Add
    BLAST

    Proteomic databases

    PRIDEiQ4UJT5.

    Interactioni

    Protein-protein interaction databases

    STRINGi315456.RF_1353.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4UJT5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.UniRule annotation

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0013.
    HOGENOMiHOG000156965.
    KOiK01872.
    OMAiYDIDIFQ.
    OrthoDBiEOG6Q2SQ2.

    Family and domain databases

    HAMAPiMF_00036_B. Ala_tRNA_synth_B.
    InterProiIPR002318. Ala-tRNA-lgiase_IIc.
    IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
    IPR018165. Ala-tRNA-synth_IIc_core.
    IPR018164. Ala-tRNA-synth_IIc_N.
    IPR023033. Ala_tRNA_ligase_euk/bac.
    IPR003156. DHHA1_dom.
    IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
    IPR009000. Transl_B-barrel.
    IPR012947. tRNA_SAD.
    [Graphical view]
    PfamiPF02272. DHHA1. 1 hit.
    PF01411. tRNA-synt_2c. 1 hit.
    PF07973. tRNA_SAD. 1 hit.
    [Graphical view]
    PRINTSiPR00980. TRNASYNTHALA.
    SMARTiSM00863. tRNA_SAD. 1 hit.
    [Graphical view]
    SUPFAMiSSF101353. SSF101353. 1 hit.
    SSF50447. SSF50447. 1 hit.
    SSF55186. SSF55186. 1 hit.
    TIGRFAMsiTIGR00344. alaS. 1 hit.
    PROSITEiPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4UJT5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKFTTEEVR SKFITYFKAN NHTHVPASSL IPHNDPSLMF VNSGMVQFKN    50
    VFTGQEKRPY NKAVTSQKSL RAGGKHNDLE NVGYTARHHT FFEMLGNFSF 100
    GDYFKEQAIY YAWNLLTKEF ELPKDKLYVT VYHTDEEAAS YWKKIAGFRD 150
    DRIIRIKTND NFWSMGDTGP CGPCSEIFYD HGEQIYGGLP GTKDEDGDRF 200
    IEIWNMVFMQ YEQIDKDTRI ELPQKSIDTG MGLERMTAVL QHVNNNYDID 250
    LFQEIINFTE NIVKVKIEGE AKFSYRVIAD HLRASSFLIA DGVIPSNEGR 300
    GYVLRRIMRR AMRHAHMLGS KEPLMYKLLP KLVDLMGNVY PELKRAESFI 350
    SSILEQEEIR FKTTLERGLK LLTEETETLT KGNELSGEIA FKLYDTYGFP 400
    LDLTEDILKN RDISVDHKRF EEQMLAQKER ARKSWLGSGE SKTDQLWFDI 450
    KEQHGSTEFL GYTLNEAECK IIALIKDNNL VNDIKEIDTQ FLLISNQTPF 500
    YGESGGQMGD IGMIFSKDSE IEVIDTLKYL GSIIVHKCIL KKGQIKIGEN 550
    ANFSIDIKYR QNLRIHHSAT HILHAVLHEV LGKHVTQKGS LVAPTYLRFD 600
    ISHSKAVTNE EITLIEDKVN EIIRDNHEVD TTLMTTEDAV KQGAMALFGE 650
    KYDSEVRVVK MGETSLELCG GTHVRRTGDI GCFKITSESA IAAGVRRIEA 700
    VCGEFVIKLM REKDSLLKSI ESSLKTNKNE LITKVNNILE RNKELEKELE 750
    KVHLARLDLS IEQIEKQAED IKGVKLIYRY IENLDNKVLR QAAENLTKKV 800
    EDLIVVYITG NNDKLSITVA VSKAITDKYK AGIIAKELSL FLGGSGGGGQ 850
    ASLAQAGGND IGKLTNIQEK LHGLLTVS 878
    Length:878
    Mass (Da):99,621
    Last modified:July 5, 2005 - v1
    Checksum:iCA941B9A40D10B98
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000053 Genomic DNA. Translation: AAY62204.1.
    RefSeqiYP_247369.1. NC_007109.1.

    Genome annotation databases

    EnsemblBacteriaiAAY62204; AAY62204; RF_1353.
    GeneIDi3400909.
    KEGGirfe:RF_1353.
    PATRICi17893638. VBIRicFel64634_1629.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000053 Genomic DNA. Translation: AAY62204.1 .
    RefSeqi YP_247369.1. NC_007109.1.

    3D structure databases

    ProteinModelPortali Q4UJT5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 315456.RF_1353.

    Proteomic databases

    PRIDEi Q4UJT5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY62204 ; AAY62204 ; RF_1353 .
    GeneIDi 3400909.
    KEGGi rfe:RF_1353.
    PATRICi 17893638. VBIRicFel64634_1629.

    Phylogenomic databases

    eggNOGi COG0013.
    HOGENOMi HOG000156965.
    KOi K01872.
    OMAi YDIDIFQ.
    OrthoDBi EOG6Q2SQ2.

    Enzyme and pathway databases

    BioCyci RFEL315456:GKEK-1390-MONOMER.

    Family and domain databases

    HAMAPi MF_00036_B. Ala_tRNA_synth_B.
    InterProi IPR002318. Ala-tRNA-lgiase_IIc.
    IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
    IPR018165. Ala-tRNA-synth_IIc_core.
    IPR018164. Ala-tRNA-synth_IIc_N.
    IPR023033. Ala_tRNA_ligase_euk/bac.
    IPR003156. DHHA1_dom.
    IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
    IPR009000. Transl_B-barrel.
    IPR012947. tRNA_SAD.
    [Graphical view ]
    Pfami PF02272. DHHA1. 1 hit.
    PF01411. tRNA-synt_2c. 1 hit.
    PF07973. tRNA_SAD. 1 hit.
    [Graphical view ]
    PRINTSi PR00980. TRNASYNTHALA.
    SMARTi SM00863. tRNA_SAD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF101353. SSF101353. 1 hit.
    SSF50447. SSF50447. 1 hit.
    SSF55186. SSF55186. 1 hit.
    TIGRFAMsi TIGR00344. alaS. 1 hit.
    PROSITEi PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
      Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
      PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC VR-1525 / URRWXCal2.

    Entry informationi

    Entry nameiSYA_RICFE
    AccessioniPrimary (citable) accession number: Q4UJT5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: July 5, 2005
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Rickettsia felis (Rickettsia azadi)
      Rickettsia felis (strain URRWXCal2): entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3