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Q4UJT5

- SYA_RICFE

UniProt

Q4UJT5 - SYA_RICFE

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Protein

Alanine--tRNA ligase

Gene

alaS

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.UniRule annotation

Catalytic activityi

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi567 – 5671ZincUniRule annotation
Metal bindingi571 – 5711ZincUniRule annotation
Metal bindingi669 – 6691ZincUniRule annotation
Metal bindingi673 – 6731ZincUniRule annotation

GO - Molecular functioni

  1. alanine-tRNA ligase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP
  3. tRNA binding Source: UniProtKB-KW
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. alanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-1390-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine--tRNA ligaseUniRule annotation (EC:6.1.1.7UniRule annotation)
Alternative name(s):
Alanyl-tRNA synthetaseUniRule annotation
Short name:
AlaRSUniRule annotation
Gene namesi
Name:alaSUniRule annotation
Ordered Locus Names:RF_1353
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000008548: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 878878Alanine--tRNA ligasePRO_0000075191Add
BLAST

Proteomic databases

PRIDEiQ4UJT5.

Interactioni

Protein-protein interaction databases

STRINGi315456.RF_1353.

Structurei

3D structure databases

ProteinModelPortaliQ4UJT5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0013.
HOGENOMiHOG000156965.
KOiK01872.
OMAiYDIDIFQ.
OrthoDBiEOG6Q2SQ2.

Family and domain databases

HAMAPiMF_00036_B. Ala_tRNA_synth_B.
InterProiIPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR003156. DHHA1_dom.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR00980. TRNASYNTHALA.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsiTIGR00344. alaS. 1 hit.
PROSITEiPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4UJT5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTKFTTEEVR SKFITYFKAN NHTHVPASSL IPHNDPSLMF VNSGMVQFKN
60 70 80 90 100
VFTGQEKRPY NKAVTSQKSL RAGGKHNDLE NVGYTARHHT FFEMLGNFSF
110 120 130 140 150
GDYFKEQAIY YAWNLLTKEF ELPKDKLYVT VYHTDEEAAS YWKKIAGFRD
160 170 180 190 200
DRIIRIKTND NFWSMGDTGP CGPCSEIFYD HGEQIYGGLP GTKDEDGDRF
210 220 230 240 250
IEIWNMVFMQ YEQIDKDTRI ELPQKSIDTG MGLERMTAVL QHVNNNYDID
260 270 280 290 300
LFQEIINFTE NIVKVKIEGE AKFSYRVIAD HLRASSFLIA DGVIPSNEGR
310 320 330 340 350
GYVLRRIMRR AMRHAHMLGS KEPLMYKLLP KLVDLMGNVY PELKRAESFI
360 370 380 390 400
SSILEQEEIR FKTTLERGLK LLTEETETLT KGNELSGEIA FKLYDTYGFP
410 420 430 440 450
LDLTEDILKN RDISVDHKRF EEQMLAQKER ARKSWLGSGE SKTDQLWFDI
460 470 480 490 500
KEQHGSTEFL GYTLNEAECK IIALIKDNNL VNDIKEIDTQ FLLISNQTPF
510 520 530 540 550
YGESGGQMGD IGMIFSKDSE IEVIDTLKYL GSIIVHKCIL KKGQIKIGEN
560 570 580 590 600
ANFSIDIKYR QNLRIHHSAT HILHAVLHEV LGKHVTQKGS LVAPTYLRFD
610 620 630 640 650
ISHSKAVTNE EITLIEDKVN EIIRDNHEVD TTLMTTEDAV KQGAMALFGE
660 670 680 690 700
KYDSEVRVVK MGETSLELCG GTHVRRTGDI GCFKITSESA IAAGVRRIEA
710 720 730 740 750
VCGEFVIKLM REKDSLLKSI ESSLKTNKNE LITKVNNILE RNKELEKELE
760 770 780 790 800
KVHLARLDLS IEQIEKQAED IKGVKLIYRY IENLDNKVLR QAAENLTKKV
810 820 830 840 850
EDLIVVYITG NNDKLSITVA VSKAITDKYK AGIIAKELSL FLGGSGGGGQ
860 870
ASLAQAGGND IGKLTNIQEK LHGLLTVS
Length:878
Mass (Da):99,621
Last modified:July 5, 2005 - v1
Checksum:iCA941B9A40D10B98
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000053 Genomic DNA. Translation: AAY62204.1.
RefSeqiYP_247369.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY62204; AAY62204; RF_1353.
GeneIDi3400909.
KEGGirfe:RF_1353.
PATRICi17893638. VBIRicFel64634_1629.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000053 Genomic DNA. Translation: AAY62204.1 .
RefSeqi YP_247369.1. NC_007109.1.

3D structure databases

ProteinModelPortali Q4UJT5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 315456.RF_1353.

Proteomic databases

PRIDEi Q4UJT5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY62204 ; AAY62204 ; RF_1353 .
GeneIDi 3400909.
KEGGi rfe:RF_1353.
PATRICi 17893638. VBIRicFel64634_1629.

Phylogenomic databases

eggNOGi COG0013.
HOGENOMi HOG000156965.
KOi K01872.
OMAi YDIDIFQ.
OrthoDBi EOG6Q2SQ2.

Enzyme and pathway databases

BioCyci RFEL315456:GKEK-1390-MONOMER.

Family and domain databases

HAMAPi MF_00036_B. Ala_tRNA_synth_B.
InterProi IPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR003156. DHHA1_dom.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view ]
Pfami PF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view ]
PRINTSi PR00980. TRNASYNTHALA.
SMARTi SM00863. tRNA_SAD. 1 hit.
[Graphical view ]
SUPFAMi SSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsi TIGR00344. alaS. 1 hit.
PROSITEi PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.

Entry informationi

Entry nameiSYA_RICFE
AccessioniPrimary (citable) accession number: Q4UJT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: July 5, 2005
Last modified: October 29, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3