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Q4UJT0

- DNAG_RICFE

UniProt

Q4UJT0 - DNAG_RICFE

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Protein

DNA primase

Gene

dnaG

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.UniRule annotation

Cofactori

Binds 1 zinc ion per monomer.UniRule annotation
Magnesium. Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi256 – 2561Magnesium 1; catalyticUniRule annotation
Metal bindingi300 – 3001Magnesium 1; catalyticUniRule annotation
Metal bindingi300 – 3001Magnesium 2UniRule annotation
Metal bindingi302 – 3021Magnesium 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri38 – 6225CHC2-typeUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA primase activity Source: InterPro
  3. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication, Transcription

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-1395-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA primaseUniRule annotation (EC:2.7.7.-UniRule annotation)
Gene namesi
Name:dnaGUniRule annotation
Ordered Locus Names:RF_1358
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000008548: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. primosome complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 595595DNA primasePRO_0000280943Add
BLAST

Interactioni

Subunit structurei

Monomer. Interacts with DnaB.UniRule annotation

Protein-protein interaction databases

STRINGi315456.RF_1358.

Structurei

3D structure databases

ProteinModelPortaliQ4UJT0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini250 – 33283ToprimUniRule annotationAdd
BLAST

Domaini

Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain.UniRule annotation

Sequence similaritiesi

Belongs to the DnaG primase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri38 – 6225CHC2-typeUniRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG0358.
HOGENOMiHOG000272927.
KOiK02316.
OMAiMMESAIT.
OrthoDBiEOG6XDGTR.

Family and domain databases

Gene3Di3.90.580.10. 1 hit.
3.90.980.10. 1 hit.
HAMAPiMF_00974. DNA_primase_DnaG.
InterProiIPR013264. DNA_primase_core_N.
IPR006295. DNA_primase_DnaG.
IPR006171. Toprim_domain.
IPR002694. Znf_CHC2.
[Graphical view]
PfamiPF13662. Toprim_4. 1 hit.
PF08275. Toprim_N. 1 hit.
PF01807. zf-CHC2. 1 hit.
[Graphical view]
PIRSFiPIRSF002811. DnaG. 1 hit.
SMARTiSM00493. TOPRIM. 1 hit.
SM00400. ZnF_CHCC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01391. dnaG. 1 hit.
PROSITEiPS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4UJT0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRVAPEFYEL LRNRINISDV VRQKVALTRK SGNYVGLCPF HQEKTPSFTV
60 70 80 90 100
SDSKRFFYCF GCKAAGDVIK FTSNISGLSY NESAIKLATD YGIEIPKLTA
110 120 130 140 150
KQKEFYEESD EILNILELAN KFFRTQLTPE ILNYLHERGI TEETVKEFSI
160 170 180 190 200
GFAPKNNKFE KFFHDKNIDI IKLGKAGLIG KRENGEIYNL FSNRITIPIR
210 220 230 240 250
NIYNKIVGFG GRVLGEGLPK YLNSPETTVF QKSETLYGEH KAISSSYKKN
260 270 280 290 300
HSILVEGYFD VIALHQAGFS EAVASLGTSV TENHLHKLWR AGDEIILCLD
310 320 330 340 350
GDNAGIKASI RTINLALPLI NSEKKISFIR LPNGLDPDDA VNKNGADFFA
360 370 380 390 400
KLIDKRISLS EMIWHIEYSG KSFKTAEEKA NLEKNLKDYC SKISDSNLKA
410 420 430 440 450
SYYRFFKEQI WQNLVTKQKK ATTKNSNLAP IISSHGYSEL EMLEHAFCAL
460 470 480 490 500
LVKFPIILEE KDIRDFILNL NFNNKSLEEF RNWYLNEIID NNVEASEITA
510 520 530 540 550
IVEKTSFFDI FLLLSKTDNL FLDISFNKNN IRLDLLWQWL HKKYYLINLQ
560 570 580 590
QEYSITINST DNHDFEKVLL YKKEILKIAN ELQVLNESFI NHTIT
Length:595
Mass (Da):68,399
Last modified:July 5, 2005 - v1
Checksum:i9B5EAA9908871984
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000053 Genomic DNA. Translation: AAY62209.1.
RefSeqiYP_247374.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY62209; AAY62209; RF_1358.
GeneIDi3400914.
KEGGirfe:RF_1358.
PATRICi17893652. VBIRicFel64634_1636.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000053 Genomic DNA. Translation: AAY62209.1 .
RefSeqi YP_247374.1. NC_007109.1.

3D structure databases

ProteinModelPortali Q4UJT0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 315456.RF_1358.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY62209 ; AAY62209 ; RF_1358 .
GeneIDi 3400914.
KEGGi rfe:RF_1358.
PATRICi 17893652. VBIRicFel64634_1636.

Phylogenomic databases

eggNOGi COG0358.
HOGENOMi HOG000272927.
KOi K02316.
OMAi MMESAIT.
OrthoDBi EOG6XDGTR.

Enzyme and pathway databases

BioCyci RFEL315456:GKEK-1395-MONOMER.

Family and domain databases

Gene3Di 3.90.580.10. 1 hit.
3.90.980.10. 1 hit.
HAMAPi MF_00974. DNA_primase_DnaG.
InterProi IPR013264. DNA_primase_core_N.
IPR006295. DNA_primase_DnaG.
IPR006171. Toprim_domain.
IPR002694. Znf_CHC2.
[Graphical view ]
Pfami PF13662. Toprim_4. 1 hit.
PF08275. Toprim_N. 1 hit.
PF01807. zf-CHC2. 1 hit.
[Graphical view ]
PIRSFi PIRSF002811. DnaG. 1 hit.
SMARTi SM00493. TOPRIM. 1 hit.
SM00400. ZnF_CHCC. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01391. dnaG. 1 hit.
PROSITEi PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.

Entry informationi

Entry nameiDNAG_RICFE
AccessioniPrimary (citable) accession number: Q4UJT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: July 5, 2005
Last modified: October 1, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3