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Q4UJT0 (DNAG_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA primase

EC=2.7.7.-
Gene names
Name:dnaG
Ordered Locus Names:RF_1358
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length595 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication By similarity. HAMAP-Rule MF_00974

Cofactor

Binds 1 zinc ion per monomer By similarity. HAMAP-Rule MF_00974

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00974

Subunit structure

Monomer By similarity. Interacts with DnaB By similarity. HAMAP-Rule MF_00974

Domain

Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain By similarity. HAMAP-Rule MF_00974

Sequence similarities

Belongs to the DnaG primase family.

Contains 1 Toprim domain.

Ontologies

Keywords
   Biological processDNA replication
Transcription
   Cellular componentDNA-directed RNA polymerase
Primosome
   DomainZinc-finger
   LigandDNA-binding
Magnesium
Metal-binding
Zinc
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentprimosome complex

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA primase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 595595DNA primase HAMAP-Rule MF_00974
PRO_0000280943

Regions

Domain250 – 33283Toprim
Zinc finger38 – 6225CHC2-type By similarity

Sites

Metal binding2561Magnesium 1; catalytic By similarity
Metal binding3001Magnesium 1; catalytic By similarity
Metal binding3001Magnesium 2 By similarity
Metal binding3021Magnesium 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4UJT0 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: 9B5EAA9908871984

FASTA59568,399
        10         20         30         40         50         60 
MRVAPEFYEL LRNRINISDV VRQKVALTRK SGNYVGLCPF HQEKTPSFTV SDSKRFFYCF 

        70         80         90        100        110        120 
GCKAAGDVIK FTSNISGLSY NESAIKLATD YGIEIPKLTA KQKEFYEESD EILNILELAN 

       130        140        150        160        170        180 
KFFRTQLTPE ILNYLHERGI TEETVKEFSI GFAPKNNKFE KFFHDKNIDI IKLGKAGLIG 

       190        200        210        220        230        240 
KRENGEIYNL FSNRITIPIR NIYNKIVGFG GRVLGEGLPK YLNSPETTVF QKSETLYGEH 

       250        260        270        280        290        300 
KAISSSYKKN HSILVEGYFD VIALHQAGFS EAVASLGTSV TENHLHKLWR AGDEIILCLD 

       310        320        330        340        350        360 
GDNAGIKASI RTINLALPLI NSEKKISFIR LPNGLDPDDA VNKNGADFFA KLIDKRISLS 

       370        380        390        400        410        420 
EMIWHIEYSG KSFKTAEEKA NLEKNLKDYC SKISDSNLKA SYYRFFKEQI WQNLVTKQKK 

       430        440        450        460        470        480 
ATTKNSNLAP IISSHGYSEL EMLEHAFCAL LVKFPIILEE KDIRDFILNL NFNNKSLEEF 

       490        500        510        520        530        540 
RNWYLNEIID NNVEASEITA IVEKTSFFDI FLLLSKTDNL FLDISFNKNN IRLDLLWQWL 

       550        560        570        580        590 
HKKYYLINLQ QEYSITINST DNHDFEKVLL YKKEILKIAN ELQVLNESFI NHTIT 

« Hide

References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000053 Genomic DNA. Translation: AAY62209.1.
RefSeqYP_247374.1. NC_007109.1.

3D structure databases

ProteinModelPortalQ4UJT0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING315456.RF_1358.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY62209; AAY62209; RF_1358.
GeneID3400914.
KEGGrfe:RF_1358.
PATRIC17893652. VBIRicFel64634_1636.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0358.
HOGENOMHOG000272927.
KOK02316.
OMAMMESAIT.
OrthoDBEOG6XDGTR.
ProtClustDBPRK05667.

Enzyme and pathway databases

BioCycRFEL315456:GKEK-1395-MONOMER.

Family and domain databases

Gene3D3.90.580.10. 1 hit.
3.90.980.10. 1 hit.
HAMAPMF_00974. DNA_primase_DnaG.
InterProIPR013264. DNA_primase_core_N.
IPR006295. DNA_primase_DnaG.
IPR006171. Toprim_domain.
IPR002694. Znf_CHC2.
[Graphical view]
PfamPF13662. Toprim_4. 1 hit.
PF08275. Toprim_N. 1 hit.
PF01807. zf-CHC2. 1 hit.
[Graphical view]
PIRSFPIRSF002811. DnaG. 1 hit.
SMARTSM00493. TOPRIM. 1 hit.
SM00400. ZnF_CHCC. 1 hit.
[Graphical view]
TIGRFAMsTIGR01391. dnaG. 1 hit.
PROSITEPS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNAG_RICFE
AccessionPrimary (citable) accession number: Q4UJT0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: July 5, 2005
Last modified: February 19, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names