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Q4UJT0

- DNAG_RICFE

UniProt

Q4UJT0 - DNAG_RICFE

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Protein
DNA primase
Gene
dnaG, RF_1358
Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication By similarity.UniRule annotation

Cofactori

Binds 1 zinc ion per monomer By similarity.UniRule annotation
Magnesium. Binds two Mg2+ per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi256 – 2561Magnesium 1; catalytic By similarity
Metal bindingi300 – 3001Magnesium 1; catalytic By similarity
Metal bindingi300 – 3001Magnesium 2 By similarity
Metal bindingi302 – 3021Magnesium 2 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri38 – 6225CHC2-type By similarity
Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA primase activity Source: InterPro
  3. zinc ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication, Transcription

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciRFEL315456:GKEK-1395-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA primase (EC:2.7.7.-)
    Gene namesi
    Name:dnaG
    Ordered Locus Names:RF_1358
    OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
    Taxonomic identifieri315456 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
    ProteomesiUP000008548: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. primosome complex Source: UniProtKB-KW
    Complete GO annotation...

    Keywords - Cellular componenti

    DNA-directed RNA polymerase, Primosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 595595DNA primaseUniRule annotation
    PRO_0000280943Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer By similarity. Interacts with DnaB By similarity.UniRule annotation

    Protein-protein interaction databases

    STRINGi315456.RF_1358.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4UJT0.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini250 – 33283Toprim
    Add
    BLAST

    Domaini

    Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain (By similarity).UniRule annotation

    Sequence similaritiesi

    Belongs to the DnaG primase family.
    Contains 1 Toprim domain.

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0358.
    HOGENOMiHOG000272927.
    KOiK02316.
    OMAiMMESAIT.
    OrthoDBiEOG6XDGTR.

    Family and domain databases

    Gene3Di3.90.580.10. 1 hit.
    3.90.980.10. 1 hit.
    HAMAPiMF_00974. DNA_primase_DnaG.
    InterProiIPR013264. DNA_primase_core_N.
    IPR006295. DNA_primase_DnaG.
    IPR006171. Toprim_domain.
    IPR002694. Znf_CHC2.
    [Graphical view]
    PfamiPF13662. Toprim_4. 1 hit.
    PF08275. Toprim_N. 1 hit.
    PF01807. zf-CHC2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002811. DnaG. 1 hit.
    SMARTiSM00493. TOPRIM. 1 hit.
    SM00400. ZnF_CHCC. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01391. dnaG. 1 hit.
    PROSITEiPS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4UJT0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRVAPEFYEL LRNRINISDV VRQKVALTRK SGNYVGLCPF HQEKTPSFTV    50
    SDSKRFFYCF GCKAAGDVIK FTSNISGLSY NESAIKLATD YGIEIPKLTA 100
    KQKEFYEESD EILNILELAN KFFRTQLTPE ILNYLHERGI TEETVKEFSI 150
    GFAPKNNKFE KFFHDKNIDI IKLGKAGLIG KRENGEIYNL FSNRITIPIR 200
    NIYNKIVGFG GRVLGEGLPK YLNSPETTVF QKSETLYGEH KAISSSYKKN 250
    HSILVEGYFD VIALHQAGFS EAVASLGTSV TENHLHKLWR AGDEIILCLD 300
    GDNAGIKASI RTINLALPLI NSEKKISFIR LPNGLDPDDA VNKNGADFFA 350
    KLIDKRISLS EMIWHIEYSG KSFKTAEEKA NLEKNLKDYC SKISDSNLKA 400
    SYYRFFKEQI WQNLVTKQKK ATTKNSNLAP IISSHGYSEL EMLEHAFCAL 450
    LVKFPIILEE KDIRDFILNL NFNNKSLEEF RNWYLNEIID NNVEASEITA 500
    IVEKTSFFDI FLLLSKTDNL FLDISFNKNN IRLDLLWQWL HKKYYLINLQ 550
    QEYSITINST DNHDFEKVLL YKKEILKIAN ELQVLNESFI NHTIT 595
    Length:595
    Mass (Da):68,399
    Last modified:July 5, 2005 - v1
    Checksum:i9B5EAA9908871984
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000053 Genomic DNA. Translation: AAY62209.1.
    RefSeqiYP_247374.1. NC_007109.1.

    Genome annotation databases

    EnsemblBacteriaiAAY62209; AAY62209; RF_1358.
    GeneIDi3400914.
    KEGGirfe:RF_1358.
    PATRICi17893652. VBIRicFel64634_1636.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000053 Genomic DNA. Translation: AAY62209.1 .
    RefSeqi YP_247374.1. NC_007109.1.

    3D structure databases

    ProteinModelPortali Q4UJT0.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 315456.RF_1358.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY62209 ; AAY62209 ; RF_1358 .
    GeneIDi 3400914.
    KEGGi rfe:RF_1358.
    PATRICi 17893652. VBIRicFel64634_1636.

    Phylogenomic databases

    eggNOGi COG0358.
    HOGENOMi HOG000272927.
    KOi K02316.
    OMAi MMESAIT.
    OrthoDBi EOG6XDGTR.

    Enzyme and pathway databases

    BioCyci RFEL315456:GKEK-1395-MONOMER.

    Family and domain databases

    Gene3Di 3.90.580.10. 1 hit.
    3.90.980.10. 1 hit.
    HAMAPi MF_00974. DNA_primase_DnaG.
    InterProi IPR013264. DNA_primase_core_N.
    IPR006295. DNA_primase_DnaG.
    IPR006171. Toprim_domain.
    IPR002694. Znf_CHC2.
    [Graphical view ]
    Pfami PF13662. Toprim_4. 1 hit.
    PF08275. Toprim_N. 1 hit.
    PF01807. zf-CHC2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002811. DnaG. 1 hit.
    SMARTi SM00493. TOPRIM. 1 hit.
    SM00400. ZnF_CHCC. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01391. dnaG. 1 hit.
    PROSITEi PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
      Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
      PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC VR-1525 / URRWXCal2.

    Entry informationi

    Entry nameiDNAG_RICFE
    AccessioniPrimary (citable) accession number: Q4UJT0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 2007
    Last sequence update: July 5, 2005
    Last modified: May 14, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Rickettsia felis (Rickettsia azadi)
      Rickettsia felis (strain URRWXCal2): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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