Q4UJT0 (PRIM_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA primase
EC=2.7.7.-

Gene names

Name:	dnaG
Ordered Locus Names:	RF_1358

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	595 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis.
Cofactor	Binds 1 zinc ion per monomer By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the DNA primase family. Contains 1 Toprim domain.

Ontologies

Keywords
Biological process	DNA replication Transcription
Cellular component	DNA-directed RNA polymerase Primosome
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	replisome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro DNA primase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 595	595	DNA primase		PRO_0000280943
Regions
	Domain	250 – 332	83	Toprim
	Zinc finger	38 – 62	25	CHC2-type By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UJT0 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 9B5EAA9908871984	FASTA	595	68,399
	10 20 30 40 50 60 MRVAPEFYEL LRNRINISDV VRQKVALTRK SGNYVGLCPF HQEKTPSFTV SDSKRFFYCF 70 80 90 100 110 120 GCKAAGDVIK FTSNISGLSY NESAIKLATD YGIEIPKLTA KQKEFYEESD EILNILELAN 130 140 150 160 170 180 KFFRTQLTPE ILNYLHERGI TEETVKEFSI GFAPKNNKFE KFFHDKNIDI IKLGKAGLIG 190 200 210 220 230 240 KRENGEIYNL FSNRITIPIR NIYNKIVGFG GRVLGEGLPK YLNSPETTVF QKSETLYGEH 250 260 270 280 290 300 KAISSSYKKN HSILVEGYFD VIALHQAGFS EAVASLGTSV TENHLHKLWR AGDEIILCLD 310 320 330 340 350 360 GDNAGIKASI RTINLALPLI NSEKKISFIR LPNGLDPDDA VNKNGADFFA KLIDKRISLS 370 380 390 400 410 420 EMIWHIEYSG KSFKTAEEKA NLEKNLKDYC SKISDSNLKA SYYRFFKEQI WQNLVTKQKK 430 440 450 460 470 480 ATTKNSNLAP IISSHGYSEL EMLEHAFCAL LVKFPIILEE KDIRDFILNL NFNNKSLEEF 490 500 510 520 530 540 RNWYLNEIID NNVEASEITA IVEKTSFFDI FLLLSKTDNL FLDISFNKNN IRLDLLWQWL 550 560 570 580 590 HKKYYLINLQ QEYSITINST DNHDFEKVLL YKKEILKIAN ELQVLNESFI NHTIT

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY62209.1.
RefSeq	YP_247374.1. NC_007109.1.
3D structure databases
HSSP	HSSP built from PDB template 1D0Q based on UniProtKB Q9X4D0.
ProteinModelPortal	Q4UJT0.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_1358.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY62209; AAY62209; RF_1358.
GeneID	3400914.
KEGG	rfe:RF_1358.
PATRIC	17893652. VBIRicFel64634_1636.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0358.
HOGENOM	HOG000272927.
KO	K02316.
OMA	PLLLEMT.
ProtClustDB	PRK05667.
Family and domain databases
Gene3D	3.90.580.10. 1 hit. 3.90.980.10. 1 hit.
InterPro	IPR013264. DNA_primase_core_N. IPR006295. DNA_primase_DnaG. IPR006171. Toprim_domain. IPR002694. Znf_CHC2. [Graphical view]
Pfam	PF13662. Toprim_4. 1 hit. PF08275. Toprim_N. 1 hit. PF01807. zf-CHC2. 1 hit. [Graphical view]
PIRSF	PIRSF002811. DnaG. 1 hit.
SMART	SM00493. TOPRIM. 1 hit. SM00400. ZnF_CHCC. 1 hit. [Graphical view]
TIGRFAMs	TIGR01391. dnaG. 1 hit.
PROSITE	PS50880. TOPRIM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRIM_RICFE

Accession

Primary (citable) accession number: Q4UJT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	July 5, 2005
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program