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Protein

DNA primase

Gene

dnaG

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Zn2+UniRule annotationNote: Binds 1 zinc ion per monomer.UniRule annotation
  • Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi256Magnesium 1; catalyticUniRule annotation1
Metal bindingi300Magnesium 1; catalyticUniRule annotation1
Metal bindingi300Magnesium 2UniRule annotation1
Metal bindingi302Magnesium 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri38 – 62CHC2-typeUniRule annotationAdd BLAST25

GO - Molecular functioni

Keywordsi

Molecular functionDNA-binding, Nucleotidyltransferase, Transferase
Biological processDNA replication, Transcription
LigandMagnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA primaseUniRule annotation (EC:2.7.7.-UniRule annotation)
Gene namesi
Name:dnaGUniRule annotation
Ordered Locus Names:RF_1358
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
Proteomesi
  • UP000008548 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

DNA-directed RNA polymerase, Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002809431 – 595DNA primaseAdd BLAST595

Interactioni

Subunit structurei

Monomer. Interacts with DnaB.UniRule annotation

Protein-protein interaction databases

STRINGi315456.RF_1358

Structurei

3D structure databases

ProteinModelPortaliQ4UJT0
SMRiQ4UJT0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini250 – 332ToprimUniRule annotationAdd BLAST83

Domaini

Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain.UniRule annotation

Sequence similaritiesi

Belongs to the DnaG primase family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri38 – 62CHC2-typeUniRule annotationAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG4105C9G Bacteria
COG0358 LUCA
HOGENOMiHOG000272927
KOiK02316
OMAiDVMEMAT
OrthoDBiPOG091H0141

Family and domain databases

CDDicd03364 TOPRIM_DnaG_primases, 1 hit
Gene3Di3.90.580.101 hit
3.90.980.101 hit
HAMAPiMF_00974 DNA_primase_DnaG, 1 hit
InterProiView protein in InterPro
IPR013264 DNA_primase_core_N
IPR037068 DNA_primase_core_N_sf
IPR006295 DNA_primase_DnaG
IPR036977 DNA_primase_Znf_CHC2
IPR030846 DnaG_bac
IPR034151 TOPRIM_DnaG_bac
IPR006171 TOPRIM_domain
IPR002694 Znf_CHC2
PfamiView protein in Pfam
PF13662 Toprim_4, 1 hit
PF08275 Toprim_N, 1 hit
PF01807 zf-CHC2, 1 hit
PIRSFiPIRSF002811 DnaG, 1 hit
SMARTiView protein in SMART
SM00493 TOPRIM, 1 hit
SM00400 ZnF_CHCC, 1 hit
TIGRFAMsiTIGR01391 dnaG, 1 hit
PROSITEiView protein in PROSITE
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

Q4UJT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVAPEFYEL LRNRINISDV VRQKVALTRK SGNYVGLCPF HQEKTPSFTV
60 70 80 90 100
SDSKRFFYCF GCKAAGDVIK FTSNISGLSY NESAIKLATD YGIEIPKLTA
110 120 130 140 150
KQKEFYEESD EILNILELAN KFFRTQLTPE ILNYLHERGI TEETVKEFSI
160 170 180 190 200
GFAPKNNKFE KFFHDKNIDI IKLGKAGLIG KRENGEIYNL FSNRITIPIR
210 220 230 240 250
NIYNKIVGFG GRVLGEGLPK YLNSPETTVF QKSETLYGEH KAISSSYKKN
260 270 280 290 300
HSILVEGYFD VIALHQAGFS EAVASLGTSV TENHLHKLWR AGDEIILCLD
310 320 330 340 350
GDNAGIKASI RTINLALPLI NSEKKISFIR LPNGLDPDDA VNKNGADFFA
360 370 380 390 400
KLIDKRISLS EMIWHIEYSG KSFKTAEEKA NLEKNLKDYC SKISDSNLKA
410 420 430 440 450
SYYRFFKEQI WQNLVTKQKK ATTKNSNLAP IISSHGYSEL EMLEHAFCAL
460 470 480 490 500
LVKFPIILEE KDIRDFILNL NFNNKSLEEF RNWYLNEIID NNVEASEITA
510 520 530 540 550
IVEKTSFFDI FLLLSKTDNL FLDISFNKNN IRLDLLWQWL HKKYYLINLQ
560 570 580 590
QEYSITINST DNHDFEKVLL YKKEILKIAN ELQVLNESFI NHTIT
Length:595
Mass (Da):68,399
Last modified:July 5, 2005 - v1
Checksum:i9B5EAA9908871984
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA Translation: AAY62209.1
RefSeqiWP_011271658.1, NC_007109.1

Genome annotation databases

EnsemblBacteriaiAAY62209; AAY62209; RF_1358
KEGGirfe:RF_1358

Similar proteinsi

Entry informationi

Entry nameiDNAG_RICFE
AccessioniPrimary (citable) accession number: Q4UJT0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: July 5, 2005
Last modified: February 28, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome