Q4UJM4 (CYB_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome b

Gene names

Name:	petB
Synonyms:	cytB
Ordered Locus Names:	RF_1009

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis By similarity.
Cofactor	Binds 2 heme groups non-covalently By similarity.
Subunit structure	The main subunits of complex b-c1 are: cytochrome b, cytochrome c1 and the Rieske protein By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Miscellaneous	Heme 1 (or BL or b562) is low-potential and absorbs at about 562 nm, and heme 2 (or BH or b566) is high-potential and absorbs at about 566 nm By similarity.
Sequence similarities	Belongs to the cytochrome b family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	respiratory electron transport chain Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Cytochrome b		PRO_0000280935
Regions
	Transmembrane	45 – 65	21	Helical; Potential
	Transmembrane	96 – 116	21	Helical; Potential
	Transmembrane	129 – 149	21	Helical; Potential
	Transmembrane	164 – 184	21	Helical; Potential
	Transmembrane	192 – 212	21	Helical; Potential
	Transmembrane	245 – 265	21	Helical; Potential
	Transmembrane	304 – 324	21	Helical; Potential
	Transmembrane	339 – 359	21	Helical; Potential
	Transmembrane	366 – 386	21	Helical; Potential
Sites
	Metal binding	95	1	Iron 1 (heme b562 axial ligand)
	Metal binding	109	1	Iron 2 (heme b566 axial ligand)
	Metal binding	196	1	Iron 1 (heme b562 axial ligand)
	Metal binding	210	1	Iron 2 (heme b566 axial ligand)

Sequences

	Sequence		Length	Mass (Da)
	Q4UJM4 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: DA27E4E4EF28599B	FASTA	398	45,684
	10 20 30 40 50 60 MNEDITPKKP NAIIEWIDYR LPIFSFLKHF SHYQTPKNLS YLWNLGSIAG IALVIQIITG 70 80 90 100 110 120 VILAMHYTPH VDHAFDSVER IMRNVNYGWL LRYTHAVGAS MFFAAVYLHI ARGLYYGSYK 130 140 150 160 170 180 APRELLWHIG IIIFLTMMAT AFMGYVLPWG QMSYWGATVI TNLFSAIPLI GESIVTWLWG 190 200 210 220 230 240 GFSVDNPTLN RFFSLHYLLP FIIVALVMLH LVALHQHGSN NPKGIDVKSP KDTIPFHPYY 250 260 270 280 290 300 TVKDFVGFGV YFIIFAYFIF YEPNYLGHPD NYVPANPLVT PAHIVPEWYF LPFYAILRAV 310 320 330 340 350 360 PSKLGGVLLM FGSIFVLFLL PWLDTSKVRS ANYRPIYRIA FWIFMTDCLL LGYLGGQPAE 370 380 390 GPYITISRFA ACYYFFHFLV ALPLIGKYEK PLPLPEEL

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY61860.1.
RefSeq	YP_247025.1. NC_007109.1.
3D structure databases
HSSP	HSSP built from PDB template 1EZV based on UniProtKB P00163.
ProteinModelPortal	Q4UJM4.
SMR	Q4UJM4. Positions 8-398.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_1009.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY61860; AAY61860; RF_1009.
GeneID	3401019.
KEGG	rfe:RF_1009.
PATRIC	17892783. VBIRicFel64634_1207.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1290.
HOGENOM	HOG000255206.
KO	K00412.
OMA	FTGWIDQ.
ProtClustDB	CLSK870835.
Family and domain databases
InterPro	IPR016175. Cyt_b/b6. IPR005798. Cyt_b/b6_C. IPR005797. Cyt_b/b6_N. IPR016174. Di-haem_cyt_TM. [Graphical view]
PANTHER	PTHR19271. PTHR19271. 1 hit.
Pfam	PF00032. Cytochrom_B_C. 1 hit. PF13631. Cytochrom_B_N_2. 1 hit. [Graphical view]
SUPFAM	SSF81648. Cytochrome_b/b6_C. 1 hit. SSF81342. Transmembr_di-haem_cytochrome. 1 hit.
PROSITE	PS51003. CYTB_CTER. 1 hit. PS51002. CYTB_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYB_RICFE

Accession

Primary (citable) accession number: Q4UJM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	July 5, 2005
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program