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Protein

Endochitinase 2

Gene

chi2

Organism
Metarhizium anisopliae (Entomophthora anisopliae)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Secreted chitinase involved in the degradation of chitin, a component of the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods). Participates in the infection process and directly acts in the penetration process of the host cuticle.1 Publication

Catalytic activityi

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei173Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Chitin degradation, Polysaccharide degradation, Virulence
LigandChitin-binding

Protein family/group databases

CAZyiCBM1. Carbohydrate-Binding Module Family 1.
GH18. Glycoside Hydrolase Family 18.
mycoCLAPiCHI18B_METAN.

Names & Taxonomyi

Protein namesi
Recommended name:
Endochitinase 2 (EC:3.2.1.14)
Alternative name(s):
Chitinase 2
Gene namesi
Name:chi2
OrganismiMetarhizium anisopliae (Entomophthora anisopliae)
Taxonomic identifieri5530 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesClavicipitaceaeMetarhizium

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Decreases the mortality of the host cotton stainer bug Dysdercus peruvianus after infection.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000042986619 – 419Endochitinase 2Add BLAST401

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi153N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi237N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi256N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Expressioni

Inductioni

Only transcribed when chitin is the carbon source. The promoter contains the consensus motif for the creA/crel/crr1 carbon catabolic repressor.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ4U4T0.
SMRiQ4U4T0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini383 – 419CBM1PROSITE-ProRule annotationAdd BLAST37

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

InterProiView protein in InterPro
IPR035971. CBD_sf.
IPR000254. Cellulose-bd_dom_fun.
IPR001223. Glyco_hydro18_cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR017853. Glycoside_hydrolase_SF.
PfamiView protein in Pfam
PF00734. CBM_1. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001821. CBD_fun. 1 hit.
SMARTiView protein in SMART
SM00236. fCBD. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF57180. SSF57180. 1 hit.
PROSITEiView protein in PROSITE
PS51164. CBM1_2. 1 hit.
PS01095. CHITINASE_18. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform chi2-2 (identifier: Q4U4T0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHHLRALVGV GLAGLAAGVP LTDKISVKPR QAPGAQNVVY WGQNGGGTIE
60 70 80 90 100
NNDLAAYCQP NSGIDVLVLA FLYQFGNGGN IPSGTIGQSC YISTSGQGQN
110 120 130 140 150
CEALTAAIHT CQSAGVKIVL SLGGATSSYS LQTQAQAEQI GQYLWDSYGN
160 170 180 190 200
SGNKTVQRPF GSNFVNGFDF DIEVNGGSSQ YYQYMIAKLR ANFASDKSNT
210 220 230 240 250
YLITGAPQCP IPEPNMGVII SNSVFDHLYV QFYNNNNYTV PCALGINGNA
260 270 280 290 300
PFNYNNWTSF IADTPSAGAK IFIGVPASPL ASTGTPSGAQ YYAAPEQLAA
310 320 330 340 350
IVGEYRSDAH FGGIMMWSAG FSDANVNDGC TYAQQAKSIL VNGAPCPSSG
360 370 380 390 400
PPSSTPATAP APTATTMPSS TSVSSPTASP TGGTVPQWGQ CGGEGYSGPT
410
QCVPPYQCVK QGDWWSSCR
Length:419
Mass (Da):43,687
Last modified:July 5, 2005 - v1
Checksum:i485A1D6116A52FCA
GO
Isoform chi2-1 (identifier: Q4U4T0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     391-419: CGGEGYSGPTQCVPPYQCVKQGDWWSSCR → VILSMCSLKKKRISRGSSHSSDV

Show »
Length:413
Mass (Da):43,026
Checksum:i2021FE08223BAC50
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_055327391 – 419CGGEG…WSSCR → VILSMCSLKKKRISRGSSHS SDV in isoform chi2-1. CuratedAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ011663 Genomic DNA. Translation: AAY34347.1.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCHI2_METAN
AccessioniPrimary (citable) accession number: Q4U4T0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 9, 2014
Last sequence update: July 5, 2005
Last modified: October 25, 2017
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families