Q4U2R8 (S22A6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Solute carrier family 22 member 6

Alternative name(s):
Organic anion transporter 1
Short name=hOAT1
PAH transporter
Short name=hPAHT
Renal organic anion transporter 1
Short name=hROAT1

Gene names

Name:	SLC22A6
Synonyms:	OAT1, PAHT

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	563 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of 2,3-dimercapto-1-propanesulfonic acid (DMPS) By similarity. Mediates the sodium-independent uptake of p-aminohippurate (PAH), ochratoxin (OTA), acyclovir (ACV), 3'-azido-3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF), cidofovir, adefovir, 9-(2-phosphonylmethoxyethyl) guanine (PMEG), 9-(2-phosphonylmethoxyethyl) diaminopurine (PMEDAP) and edaravone sulfate. PAH uptake is inhibited by p-chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), sulindac, diclofenac, carprofen, glutarate and okadaic acid By similarity. PAH uptake is inhibited by benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, furosemide, indomethacin, bumetamide, losartan, probenecid, phenol red, urate, and alpha-ketoglutarate. Ref.1 Ref.2 Ref.11 Ref.14 Ref.16 Ref.17
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Tissue specificity	Strongly expressed in kidney and to a lower extent in liver, skeletal muscle, brain and placenta. Found at the basolateral membrane of the proximal tubule. Ref.2 Ref.3 Ref.4 Ref.5 Ref.6
Domain	Multiple cysteine residues are necessary for proper targeting to the plasma membrane By similarity.
Post-translational modification	Glycosylated. Glycosylation at Asn-113 may occur at a secondary level. Glycosylation is necessary for proper targeting of the transporter to the plasma membrane. Ref.5 Ref.12
Sequence similarities	Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. [View classification]
Biophysicochemical properties	Kinetic parameters: KM=9.3 µM for PAH (isoform 1) Ref.5 Ref.6 Ref.11 Ref.15 KM=4 µM for PAH (isoform 2) KM=11 µM for edaravone KM=46 µM for cidofovir KM=30 µM for adefovir KM=5.77 µM for 2,4-D KM=23.5 µM for HA KM=14 µM for IA KM=20.5 µM for IS KM=141 µM for CMPF Vmax=534 pmol/min/mg enzyme for 2,4-D uptake Vmax=430 pmol/min/mg enzyme for HA uptake Vmax=110 pmol/min/mg enzyme for IA uptake Vmax=216 pmol/min/mg enzyme for IS uptake Vmax=801 pmol/min/mg enzyme for CMPF uptake

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	alpha-ketoglutarate transport Inferred from direct assay Ref.3. Source: UniProtKB renal tubular secretion Inferred from mutant phenotype PubMed 22169006. Source: UniProtKB response to methotrexate Inferred from electronic annotation. Source: Compara sodium-independent organic anion transport Inferred from direct assay Ref.2. Source: UniProtKB
Cellular_component	basolateral plasma membrane Inferred from direct assay PubMed 15037815Ref.2. Source: UniProtKB caveola Inferred from electronic annotation. Source: Compara integral to plasma membrane Inferred from direct assay Ref.12. Source: UniProtKB protein complex Inferred from electronic annotation. Source: Compara
Molecular_function	inorganic anion exchanger activity Inferred from direct assay PubMed 15037815. Source: UniProtKB organic anion transmembrane transporter activity Inferred from direct assay Ref.4Ref.3. Source: UniProtKB sodium-independent organic anion transmembrane transporter activity Inferred from direct assay Ref.2. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APPBP2	Q92624	3	EBI-749741,EBI-743771

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q4U2R8-1)

Also known as: OAT1-1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q4U2R8-2)

Also known as: OAT1-2;

The sequence of this isoform differs from the canonical sequence as follows:
     523-535: Missing.

Isoform 3 (identifier: Q4U2R8-3)

Also known as: OAT1-3;

The sequence of this isoform differs from the canonical sequence as follows:
     455-498: Missing.
     523-535: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q4U2R8-4)

Also known as: OAT1-4;

The sequence of this isoform differs from the canonical sequence as follows:
     455-498: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 563	563	Solute carrier family 22 member 6		PRO_0000324166
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 30	21	Helical; Potential
	Topological domain	31 – 135	105	Extracellular Potential
	Transmembrane	136 – 156	21	Helical; Potential
	Topological domain	157 – 164	8	Cytoplasmic Potential
	Transmembrane	165 – 187	23	Helical; Potential
	Topological domain	188 – 190	3	Extracellular Potential
	Transmembrane	191 – 213	23	Helical; Potential
	Topological domain	214 – 224	11	Cytoplasmic Potential
	Transmembrane	225 – 245	21	Helical; Potential
	Topological domain	246 – 248	3	Extracellular Potential
	Transmembrane	249 – 269	21	Helical; Potential
	Topological domain	270 – 337	68	Cytoplasmic Potential
	Transmembrane	338 – 358	21	Helical; Potential
	Topological domain	359 – 368	10	Extracellular Potential
	Transmembrane	369 – 389	21	Helical; Potential
	Topological domain	390 – 395	6	Cytoplasmic Potential
	Transmembrane	396 – 416	21	Helical; Potential
	Topological domain	417 – 425	9	Extracellular Potential
	Transmembrane	426 – 446	21	Helical; Potential
	Topological domain	447 – 455	9	Cytoplasmic Potential
	Transmembrane	456 – 475	20	Helical; Potential
	Topological domain	476 – 484	9	Extracellular Potential
	Transmembrane	485 – 505	21	Helical; Potential
	Topological domain	506 – 563	58	Cytoplasmic Potential
Sites
	Site	230	1	Important for interaction with cidofovir
	Site	438	1	Important for interaction with cidofovir and PAH
Amino acid modifications
	Glycosylation	39	1	N-linked (GlcNAc...) Ref.12
	Glycosylation	56	1	N-linked (GlcNAc...) Ref.12
	Glycosylation	92	1	N-linked (GlcNAc...) Ref.12
	Glycosylation	97	1	N-linked (GlcNAc...) Ref.12
	Glycosylation	113	1	N-linked (GlcNAc...)
Natural variations
	Alternative sequence	455 – 498	44	Missing in isoform 3 and isoform 4.		VSP_032168
	Alternative sequence	523 – 535	13	Missing in isoform 2 and isoform 3.		VSP_032169
	Natural variant	7	1	L → P. Ref.19		VAR_039682
	Natural variant	50	1	R → H Lower Vmax; increase in substrate affinity and increase in the affinity for the nucleoside phosphonate analogs cidofovir, adefovir and tenofovir. Ref.18 Ref.19 Corresponds to variant rs11568626 [ dbSNP | Ensembl ].		VAR_039683
	Natural variant	104	1	P → L. Ref.7 Corresponds to variant rs11568627 [ dbSNP | Ensembl ].		VAR_047878
	Natural variant	293	1	R → W Increase in substrate affinity. Corresponds to variant rs45607933 [ dbSNP | Ensembl ].		VAR_039684
Experimental info
	Mutagenesis	30	1	L → A: Complete loss of PAH transport activity. Ref.13
	Mutagenesis	36	1	T → A: Complete loss of PAH transport activity. Ref.13
	Mutagenesis	39	1	N → Q: Complete loss of PAH transport activity. Ref.12
	Mutagenesis	230	1	Y → A: Loss of membrane protein expression and little uptake of cidofovir. Ref.16
	Mutagenesis	431	1	K → A: Decrease in the level of membrane protein expression and 70 % loss of PAH uptake. Ref.16
	Mutagenesis	438	1	F → A: Decrease in the level of membrane protein expression, 70 % loss of PAH uptake, increased affinity for cidofovir, lower Vmax for PAH, and lower Km and Vmax for cidofovir. Ref.16
	Sequence conflict	14	1	G → S in AAD10052. Ref.3
	Sequence conflict	563	1	L → F in AAC70004. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (OAT1-1) [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 74AD3EA2678032E4	FASTA	563	61,816
	10 20 30 40 50 60 MAFNDLLQQV GGVGRFQQIQ VTLVVLPLLL MASHNTLQNF TAAIPTHHCR PPADANLSKN 70 80 90 100 110 120 GGLEVWLPRD RQGQPESCLR FTSPQWGLPF LNGTEANGTG ATEPCTDGWI YDNSTFPSTI 130 140 150 160 170 180 VTEWDLVCSH RALRQLAQSL YMVGVLLGAM VFGYLADRLG RRKVLILNYL QTAVSGTCAA 190 200 210 220 230 240 FAPNFPIYCA FRLLSGMALA GISLNCMTLN VEWMPIHTRA CVGTLIGYVY SLGQFLLAGV 250 260 270 280 290 300 AYAVPHWRHL QLLVSAPFFA FFIYSWFFIE SARWHSSSGR LDLTLRALQR VARINGKREE 310 320 330 340 350 360 GAKLSMEVLR ASLQKELTMG KGQASAMELL RCPTLRHLFL CLSMLWFATS FAYYGLVMDL 370 380 390 400 410 420 QGFGVSIYLI QVIFGAVDLP AKLVGFLVIN SLGRRPAQMA ALLLAGICIL LNGVIPQDQS 430 440 450 460 470 480 IVRTSLAVLG KGCLAASFNC IFLYTGELYP TMIRQTGMGM GSTMARVGSI VSPLVSMTAE 490 500 510 520 530 540 LYPSMPLFIY GAVPVAASAV TVLLPETLGQ PLPDTVQDLE SRWAPTQKEA GIYPRKGKQT 550 560 RQQQEHQKYM VPLQASAQEK NGL
	Isoform 2 (OAT1-2) [UniParc]. Checksum: BC5D6DBDD0072D92	FASTA	550	60,318
	10 20 30 40 50 60 MAFNDLLQQV GGVGRFQQIQ VTLVVLPLLL MASHNTLQNF TAAIPTHHCR PPADANLSKN 70 80 90 100 110 120 GGLEVWLPRD RQGQPESCLR FTSPQWGLPF LNGTEANGTG ATEPCTDGWI YDNSTFPSTI 130 140 150 160 170 180 VTEWDLVCSH RALRQLAQSL YMVGVLLGAM VFGYLADRLG RRKVLILNYL QTAVSGTCAA 190 200 210 220 230 240 FAPNFPIYCA FRLLSGMALA GISLNCMTLN VEWMPIHTRA CVGTLIGYVY SLGQFLLAGV 250 260 270 280 290 300 AYAVPHWRHL QLLVSAPFFA FFIYSWFFIE SARWHSSSGR LDLTLRALQR VARINGKREE 310 320 330 340 350 360 GAKLSMEVLR ASLQKELTMG KGQASAMELL RCPTLRHLFL CLSMLWFATS FAYYGLVMDL 370 380 390 400 410 420 QGFGVSIYLI QVIFGAVDLP AKLVGFLVIN SLGRRPAQMA ALLLAGICIL LNGVIPQDQS 430 440 450 460 470 480 IVRTSLAVLG KGCLAASFNC IFLYTGELYP TMIRQTGMGM GSTMARVGSI VSPLVSMTAE 490 500 510 520 530 540 LYPSMPLFIY GAVPVAASAV TVLLPETLGQ PLPDTVQDLE SRKGKQTRQQ QEHQKYMVPL 550 QASAQEKNGL
	Isoform 3 (OAT1-3) [UniParc]. Checksum: D8EBAE8A113E6C5E	FASTA	506	55,858
	10 20 30 40 50 60 MAFNDLLQQV GGVGRFQQIQ VTLVVLPLLL MASHNTLQNF TAAIPTHHCR PPADANLSKN 70 80 90 100 110 120 GGLEVWLPRD RQGQPESCLR FTSPQWGLPF LNGTEANGTG ATEPCTDGWI YDNSTFPSTI 130 140 150 160 170 180 VTEWDLVCSH RALRQLAQSL YMVGVLLGAM VFGYLADRLG RRKVLILNYL QTAVSGTCAA 190 200 210 220 230 240 FAPNFPIYCA FRLLSGMALA GISLNCMTLN VEWMPIHTRA CVGTLIGYVY SLGQFLLAGV 250 260 270 280 290 300 AYAVPHWRHL QLLVSAPFFA FFIYSWFFIE SARWHSSSGR LDLTLRALQR VARINGKREE 310 320 330 340 350 360 GAKLSMEVLR ASLQKELTMG KGQASAMELL RCPTLRHLFL CLSMLWFATS FAYYGLVMDL 370 380 390 400 410 420 QGFGVSIYLI QVIFGAVDLP AKLVGFLVIN SLGRRPAQMA ALLLAGICIL LNGVIPQDQS 430 440 450 460 470 480 IVRTSLAVLG KGCLAASFNC IFLYTGELYP TMIRAVTVLL PETLGQPLPD TVQDLESRKG 490 500 KQTRQQQEHQ KYMVPLQASA QEKNGL
	Isoform 4 (OAT1-4) [UniParc]. Checksum: E1748C6F9E2002F2	FASTA	519	57,357
	10 20 30 40 50 60 MAFNDLLQQV GGVGRFQQIQ VTLVVLPLLL MASHNTLQNF TAAIPTHHCR PPADANLSKN 70 80 90 100 110 120 GGLEVWLPRD RQGQPESCLR FTSPQWGLPF LNGTEANGTG ATEPCTDGWI YDNSTFPSTI 130 140 150 160 170 180 VTEWDLVCSH RALRQLAQSL YMVGVLLGAM VFGYLADRLG RRKVLILNYL QTAVSGTCAA 190 200 210 220 230 240 FAPNFPIYCA FRLLSGMALA GISLNCMTLN VEWMPIHTRA CVGTLIGYVY SLGQFLLAGV 250 260 270 280 290 300 AYAVPHWRHL QLLVSAPFFA FFIYSWFFIE SARWHSSSGR LDLTLRALQR VARINGKREE 310 320 330 340 350 360 GAKLSMEVLR ASLQKELTMG KGQASAMELL RCPTLRHLFL CLSMLWFATS FAYYGLVMDL 370 380 390 400 410 420 QGFGVSIYLI QVIFGAVDLP AKLVGFLVIN SLGRRPAQMA ALLLAGICIL LNGVIPQDQS 430 440 450 460 470 480 IVRTSLAVLG KGCLAASFNC IFLYTGELYP TMIRAVTVLL PETLGQPLPD TVQDLESRWA 490 500 510 PTQKEAGIYP RKGKQTRQQQ EHQKYMVPLQ ASAQEKNGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a human renal p-aminohippurate transporter, hROAT1." Reid G., Wolff N.A., Dautzenberg F.M., Burckhardt G. Kidney Blood Press. Res. 21:233-237(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION.
[2]	"Molecular cloning and functional expression of a multispecific organic anion transporter from human kidney." Hosoyamada M., Sekine T., Kanai Y., Endou H. Am. J. Physiol. 276:F122-F128(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, FUNCTION. Tissue: Kidney.
[3]	"Cloning of the human kidney PAH transporter: narrow substrate specificity and regulation by protein kinase C." Lu R., Chan B.S., Schuster V.L. Am. J. Physiol. 276:F295-F303(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY. Tissue: Kidney.
[4]	"Molecular cloning and characterization of two novel human renal organic anion transporters (hOAT1 and hOAT3)." Race J.E., Grassl S.M., Williams W.J., Holtzman E.J. Biochem. Biophys. Res. Commun. 255:508-514(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY. Tissue: Kidney.
[5]	"The antiviral nucleotide analogs cidofovir and adefovir are novel substrates for human and rat renal organic anion transporter 1." Cihlar T., Lin D.C., Pritchard J.B., Fuller M.D., Mendel D.B., Sweet D.H. Mol. Pharmacol. 56:570-580(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), GLYCOSYLATION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY. Tissue: Kidney.
[6]	"Genomic structure and in vivo expression of the human organic anion transporter 1 (hOAT1) gene." Bahn A., Prawitt D., Buttler D., Reid G., Enklaar T., Wolff N.A., Ebbinghaus C., Hillemann A., Schulten H.-J., Gunawan B., Fuezesi L., Zabel B., Burckhardt G. Biochem. Biophys. Res. Commun. 275:623-630(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 3 AND 4), BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY. Tissue: Kidney.
[7]	NIEHS SNPs program Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT LEU-104.
[8]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Colon.
[11]	"Interaction of cysteine conjugates with human and rabbit organic anion transporter 1." Groves C.E., Munoz L., Bahn A., Burckhardt G., Wright S.H. J. Pharmacol. Exp. Ther. 304:560-566(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[12]	"Role of glycosylation in the organic anion transporter OAT1." Tanaka K., Xu W., Zhou F., You G. J. Biol. Chem. 279:14961-14966(2004) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF ASN-39, GLYCOSYLATION AT ASN-39; ASN-56; ASN-92 AND ASN-97.
[13]	"Critical amino acid residues in transmembrane domain 1 of the human organic anion transporter hOAT1." Hong M., Zhou F., You G. J. Biol. Chem. 279:31478-31482(2004) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF LEU-30 AND THR-36.
[14]	"Transport of the natural sweetener stevioside and its aglycone steviol by human organic anion transporter (hOAT1; SLC22A6) and hOAT3 (SLC22A8)." Srimaroeng C., Chatsudthipong V., Aslamkhan A.G., Pritchard J.B. J. Pharmacol. Exp. Ther. 313:621-628(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[15]	"Molecular cloning and functional analyses of OAT1 and OAT3 from cynomolgus monkey kidney." Tahara H., Shono M., Kusuhara H., Kinoshita H., Fuse E., Takadate A., Otagiri M., Sugiyama Y. Pharm. Res. 22:647-660(2005) [PubMed] [Europe PMC] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
[16]	"A three-dimensional model of human organic anion transporter 1: aromatic amino acids required for substrate transport." Perry J.L., Dembla-Rajpal N., Hall L.A., Pritchard J.B. J. Biol. Chem. 281:38071-38079(2006) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF TYR-230; LYS-431 AND PHE-438, SUBSTRATE-BINDING SITES, FUNCTION.
[17]	"Human organic anion transporters 1 (hOAT1/SLC22A6) and 3 (hOAT3/SLC22A8) transport edaravone (MCI-186; 3-methyl-1-phenyl-2-pyrazolin-5-one) and its sulfate conjugate." Mizuno N., Takahashi T., Iwase Y., Kusuhara H., Niwa T., Sugiyama Y. Drug Metab. Dispos. 35:1429-1434(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[18]	"Functional consequences of single nucleotide polymorphisms in the human organic anion transporter hOAT1 (SLC22A6)." Bleasby K., Hall L.A., Perry J.L., Mohrenweiser H.W., Pritchard J.B. J. Pharmacol. Exp. Ther. 314:923-931(2005) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT HIS-50, CHARACTERIZATION OF VARIANT HIS-50.
[19]	"Analyses of coding region polymorphisms in apical and basolateral human organic anion transporter (OAT) genes [OAT1 (NKT), OAT2, OAT3, OAT4, URAT (RST)]." Xu G., Bhatnagar V., Wen G., Hamilton B.A., Eraly S.A., Nigam S.K. Kidney Int. 68:1491-1499(2005) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS PRO-7 AND HIS-50.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF057039 mRNA. Translation: AAC70004.1. AB009697 mRNA. Translation: BAA75072.1. AB009698 mRNA. Translation: BAA75073.1. AF104038 mRNA. Translation: AAD10052.1. AF097490 mRNA. Translation: AAD19356.1. AF124373 mRNA. Translation: AAD55356.1. AJ249369 Genomic DNA. Translation: CAB77184.1. AJ251529 mRNA. Translation: CAB94830.1. AJ271205 mRNA. Translation: CAB97249.1. EU567146 Genomic DNA. Translation: ACB21049.1. AP001858 Genomic DNA. No translation available. CH471076 Genomic DNA. Translation: EAW74129.1. CH471076 Genomic DNA. Translation: EAW74130.1. CH471076 Genomic DNA. Translation: EAW74131.1. CH471076 Genomic DNA. Translation: EAW74132.1. BC033682 mRNA. Translation: AAH33682.1.
IPI	IPI00006296. IPI00011035. IPI00645624. IPI00654872.
RefSeq	NP_004781.2. NM_004790.4. NP_695008.1. NM_153276.2. NP_695009.1. NM_153277.2. NP_695010.1. NM_153278.2.
UniGene	Hs.369252.
3D structure databases
ProteinModelPortal	Q4U2R8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q4U2R8. 1 interaction.
MINT	MINT-1474237.
Polymorphism databases
DMDM	74762955.
Proteomic databases
PaxDb	Q4U2R8.
PRIDE	Q4U2R8.
Protocols and materials databases
DNASU	9356.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000360421; ENSP00000353597; ENSG00000197901. ENST00000377871; ENSP00000367102; ENSG00000197901. ENST00000421062; ENSP00000404441; ENSG00000197901. ENST00000458333; ENSP00000396401; ENSG00000197901.
GeneID	9356.
KEGG	hsa:9356.
UCSC	uc001nwj.3. human. uc001nwk.3. human. uc001nwl.3. human. uc001nwm.3. human.
Organism-specific databases
CTD	9356.
GeneCards	GC11M062744.
HGNC	HGNC:10970. SLC22A6.
MIM	607582. gene.
neXtProt	NX_Q4U2R8.
PharmGKB	PA388.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0477.
HOVERGEN	HBG108433.
InParanoid	Q4U2R8.
KO	K08203.
OMA	APFFAFF.
OrthoDB	EOG418BPG.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules.
Gene expression databases
ArrayExpress	Q4U2R8.
Bgee	Q4U2R8.
Genevestigator	Q4U2R8.
Family and domain databases
InterPro	IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. IPR004749. Orgcat_transp. IPR005828. Sub_transporter. [Graphical view]
Pfam	PF00083. Sugar_tr. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00898. 2A0119. 1 hit.
PROSITE	PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q4U2R8.
ChEMBL	CHEMBL1641347.
GenomeRNAi	9356.
NextBio	35037.
SOURCE	Search...

Entry information

Entry name

S22A6_HUMAN

Accession

Primary (citable) accession number: Q4U2R8
Secondary accession number(s): A8MY93 , B2D0R6, O95187, O95742, Q7LDA0, Q8N192, Q9NQA6, Q9NQC2, Q9UBG6, Q9UEQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	July 19, 2005
Last modified:	April 3, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.