Reviewed, UniProtKB/Swiss-Prot Q4TWH9 (NS1A_HASV5)

Names and origin

Protein names

Recommended name:
    Non-structural polyprotein 1A
Cleaved into the following 4 chains:
    1- Recommended name:
            Protein p19
    2- Recommended name:
            Transmembrane protein 1A
    3- Recommended name:
            Serine protease p27
                Short name=p27
              EC=3.4.21.-
    4- Recommended name:
            Protein p20'

Gene names

Name:

ORF1

Organism

Human astrovirus-5 (HAstV-5)

Taxonomic identifier

35741 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Astroviridae › Mamastrovirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	920 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Non-structural polyprotein 1A encodes for protease and some functions involved in viral replication/transcription.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Host membrane; Multi-pass membrane protein Potential.
Post-translational modification	Cleaved presumably by viral and host proteases.
Sequence similarities	Belongs to the astroviridae polyprotein 1A family.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Membrane
Coding sequence diversity	Ribosomal frameshifting
Domain	Coiled coil Transmembrane
Molecular function	Hydrolase Protease Thiol protease
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]

Isoform nsp1a (identifier: Q4TWH9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform nsp1ab (identifier: Q4TWH8-1)

The sequence of this isoform can be found in the external entry Q4TWH8-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Generated by a ribosomal frameshift at position 905.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 920	920	Non-structural polyprotein 1A		PRO_0000327335
	Chain	1 – 175	175	Protein p19 Potential		PRO_0000327336
	Chain	176 – 419	244	Transmembrane protein 1A Potential		PRO_0000327337
	Chain	420 – 664	245	Serine protease p27 Potential		PRO_0000327338
	Chain	665 – 920	256	Protein p20' Potential		PRO_0000327339
Regions
	Transmembrane	239 – 259	21	Potential
	Transmembrane	286 – 306	21	Potential
	Transmembrane	313 – 333	21	Potential
	Transmembrane	344 – 364	21	Potential
Sites
	Active site	461	1	Charge relay system; for serine protease activity By similarity
	Active site	489	1	Charge relay system; for serine protease activity By similarity
	Active site	551	1	Charge relay system; for serine protease activity By similarity
	Site	175 – 176	2	Cleavage Potential
	Site	419 – 420	2	Cleavage Potential
	Site	664 – 665	2	Cleavage Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform nsp1a [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 6EB457F9C65B49F4	FASTA	920	103,531
	10 20 30 40 50 60 MAHGEPYYSS KPDKDFNFGS TMARRQMTPT MVTKLPKFVR NSPQVYDWIV RGLIFPTTGK 70 80 90 100 110 120 TYFQRVVVIT GGFEDGTYGS FAFDGREWVE IYPIEHLNLM SSLKLIHKAN ALQERLRLSQ 130 140 150 160 170 180 EEKATLTLDV QFLQHENVRL KELISKPEPR KIQMKWIIVG AVLTFLSLIP GGYAQSQTNN 190 200 210 220 230 240 TIFTDVIAAC KYSTETLTEN LDLRIKLALA NITISDKLDA VRQILNFAFV PRSHWLRTVF 250 260 270 280 290 300 YYIHYYEMWN IFMFVLAIGT VMRSTRPGTD LITLATSHLS GFRMAVLPTI PFHTTMTLWV 310 320 330 340 350 360 MNTLMVCYYF DNLLAITMAI LAPILGIIFL CFMEDSNYVS QIRGLIATAV LIAGGHACLT 370 380 390 400 410 420 LTGTTTSLFV VILTCRFVRM ATIFIGTRFE IRDANGKVVA TVPTRIKNAA FDFFQRLKQS 430 440 450 460 470 480 GVRVGVNEFV VIKPGALCVI DTPEGKGTGF FSGNDIVTAA HVVGNNTFVN VCYEGLMYEA 490 500 510 520 530 540 KVRYMPEKDI AFITCPGDLH PTARLKLSKN PDYSCVTVMA YVNEDLVVST AAAMVHGNTL 550 560 570 580 590 600 SYAVRTQDGM SGAPVCDKYG RVLAVHQTNT GYTGGAVIID PADFHPVKAP SQVELLKEEI 610 620 630 640 650 660 ERLKAQLNSA AENPSTVITQ QPTATLEQKS VNDSDVVDLV RTAMEREMKI LRDEINGILA 670 680 690 700 710 720 PFLQKKKGKT KHGRGRVRRN LRKGVKLLTE EEYRELLEKG LDRETFLDLI DRIIGERSGY 730 740 750 760 770 780 PDYDDEDYYD EDDDGWGMVG DDVEFDYTEV INFDQAKPTP APRTTKPKPC PEPEAETQPL 790 800 810 820 830 840 DLSQKKDKQL EHEQQVVKPT KPQKNDPQPY SQTYGKAPIW ESYDFDWDED DAKFILPAPP 850 860 870 880 890 900 RLTKADEIVL GSKIVKLRTI IETAIKTQNY SALPEAVFEL DKAAYEAGLE GFLQRVKSKN 910 920 KAPKNYKGPQ KTKGPKTIIH
	Isoform nsp1ab. See Q4TWH8.	FASTA	–	–

References

[1]

"Molecular characterization of human astroviruses isolated in Brazil, including the complete sequences of astrovirus genotypes 4 and 5." Silva P.A., Cardoso D.D., Schreier E. Arch. Virol. 151:1405-1417(2006) [PubMed: 16421636] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Goiania/GO/12/94/Brazil.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ028633 Genomic RNA. Translation: AAY46272.1.
3D structure databases
ModBase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

NS1A_HASV5

Accession

Primary (citable) accession number: Q4TWH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	July 19, 2005
Last modified:	May 5, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)