Reviewed, UniProtKB/Swiss-Prot Q4TWH8 (NS1AB_HASV5)

Names and origin

Protein names

Recommended name:
    Non-structural polyprotein 1AB
Cleaved into the following 5 chains:
    1- Recommended name:
            Protein p19
    2- Recommended name:
            Transmembrane protein 1A
    3- Recommended name:
            Serine protease p27
                Short name=p27
              EC=3.4.21.-
    4- Recommended name:
            Protein p20
    5- Recommended name:
            RNA-directed RNA polymerase p57
                Short name=p57
              EC=2.7.7.48

Gene names

Name:

ORF1

Organism

Human astrovirus-5 (HAstV-5)

Taxonomic identifier

35741 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Astroviridae › Mamastrovirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	1416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Non-structural polyprotein 1AB contains the viral protease participating in the cleavage of the polyprotein into functional products. It contains also the activities necessary for replication of genomic RNA, as well as transcription of subgenomic mRNA By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Host membrane; Multi-pass membrane protein Potential.
Post-translational modification	Cleaved presumably by viral and host proteases By similarity.
Sequence similarities	Belongs to the astroviridae polyprotein 1AB family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Membrane
Coding sequence diversity	Ribosomal frameshifting
Domain	Coiled coil Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Nucleotidyltransferase Protease RNA-directed RNA polymerase Thiol protease Transferase
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]

Isoform nsp1ab (identifier: Q4TWH8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Generated by a ribosomal frameshift at position 905.

Isoform nsp1a (identifier: Q4TWH9-1)

The sequence of this isoform can be found in the external entry Q4TWH9-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1416	1416	Non-structural polyprotein 1AB		PRO_0000327283
	Chain	1 – 175	175	Protein p19 Potential		PRO_0000327284
	Chain	176 – 419	244	Transmembrane protein 1A Potential		PRO_0000327285
	Chain	420 – 664	245	Serine protease p27 Potential		PRO_0000327286
	Chain	665 – 913	249	Protein p20 Potential		PRO_0000327287
	Chain	914 – 1416	503	RNA-directed RNA polymerase p57 Potential		PRO_0000327288
Regions
	Transmembrane	154 – 174	21	Potential
	Transmembrane	239 – 259	21	Potential
	Transmembrane	286 – 306	21	Potential
	Transmembrane	313 – 333	21	Potential
	Transmembrane	344 – 364	21	Potential
	Domain	1161 – 1287	127	RdRp catalytic
	Coiled coil	104 – 146	43	Potential
	Coiled coil	587 – 614	28	Potential
Sites
	Active site	461	1	Charge relay system; for serine protease activity By similarity
	Active site	489	1	Charge relay system; for serine protease activity By similarity
	Active site	551	1	Charge relay system; for serine protease activity By similarity
	Site	175 – 176	2	Cleavage Potential
	Site	419 – 420	2	Cleavage Potential
	Site	664 – 665	2	Cleavage Potential
	Site	913 – 914	2	Cleavage Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform nsp1ab [UniParc]. Last modified April 8, 2008. Version 2. Checksum: CB75E8DC9B60DE0D	FASTA	1,416	161,728
	10 20 30 40 50 60 MAHGEPYYSS KPDKDFNFGS TMARRQMTPT MVTKLPKFVR NSPQVYDWIV RGLIFPTTGK 70 80 90 100 110 120 TYFQRVVVIT GGFEDGTYGS FAFDGREWVE IYPIEHLNLM SSLKLIHKAN ALQERLRLSQ 130 140 150 160 170 180 EEKATLTLDV QFLQHENVRL KELISKPEPR KIQMKWIIVG AVLTFLSLIP GGYAQSQTNN 190 200 210 220 230 240 TIFTDVIAAC KYSTETLTEN LDLRIKLALA NITISDKLDA VRQILNFAFV PRSHWLRTVF 250 260 270 280 290 300 YYIHYYEMWN IFMFVLAIGT VMRSTRPGTD LITLATSHLS GFRMAVLPTI PFHTTMTLWV 310 320 330 340 350 360 MNTLMVCYYF DNLLAITMAI LAPILGIIFL CFMEDSNYVS QIRGLIATAV LIAGGHACLT 370 380 390 400 410 420 LTGTTTSLFV VILTCRFVRM ATIFIGTRFE IRDANGKVVA TVPTRIKNAA FDFFQRLKQS 430 440 450 460 470 480 GVRVGVNEFV VIKPGALCVI DTPEGKGTGF FSGNDIVTAA HVVGNNTFVN VCYEGLMYEA 490 500 510 520 530 540 KVRYMPEKDI AFITCPGDLH PTARLKLSKN PDYSCVTVMA YVNEDLVVST AAAMVHGNTL 550 560 570 580 590 600 SYAVRTQDGM SGAPVCDKYG RVLAVHQTNT GYTGGAVIID PADFHPVKAP SQVELLKEEI 610 620 630 640 650 660 ERLKAQLNSA AENPSTVITQ QPTATLEQKS VNDSDVVDLV RTAMEREMKI LRDEINGILA 670 680 690 700 710 720 PFLQKKKGKT KHGRGRVRRN LRKGVKLLTE EEYRELLEKG LDRETFLDLI DRIIGERSGY 730 740 750 760 770 780 PDYDDEDYYD EDDDGWGMVG DDVEFDYTEV INFDQAKPTP APRTTKPKPC PEPEAETQPL 790 800 810 820 830 840 DLSQKKDKQL EHEQQVVKPT KPQKNDPQPY SQTYGKAPIW ESYDFDWDED DAKFILPAPP 850 860 870 880 890 900 RLTKADEIVL GSKIVKLRTI IETAIKTQNY SALPEAVFEL DKAAYEAGLE GFLQRVKSKN 910 920 930 940 950 960 KAPKKLQRAP EDQGAQNYHS LDAWKSLLEP PRERRCVPAN FPLLGHLPID RPIFDDKKPR 970 980 990 1000 1010 1020 DDLLGLLPEP TWHAFEEYGP TTWGPQAFIK SFDKFFYAEP IDFFSEYPQL CAFADWATYR 1030 1040 1050 1060 1070 1080 EFRYLEDTRV IHITATEKNT DSTPAYPKMN YFDTEEKYLE SYGWAPYIRE FTRVFKGDKP 1090 1100 1110 1120 1130 1140 EVLWYLFLKK EIIKEEKIRN SDIRQIVCAD PIYTRIGACL EAHQNALMKQ HTETSVGQCG 1150 1160 1170 1180 1190 1200 WSPMEGGFKK TMQRLVNKGN KYFIEFDWTR YDGTIPPSLF RHIKEIRWNF INKDQREKYR 1210 1220 1230 1240 1250 1260 HVHEWYVDNL LNRHVLLPSG EVTLQTRGNP SGQFSTTMDN NMINFWLQAF EFAYFNGPNK 1270 1280 1290 1300 1310 1320 DLWKTYDTVV YGDDRLSTTP SVPENYEERV IDMYRDIFGM WVKPGKVICR ESIVGLSFCG 1330 1340 1350 1360 1370 1380 FTVNADLEPV PTSPEKLMAS LLKPYKILPD LESLHGKLLC YQLLAAFMAE DHPFKVYVEH 1390 1400 1410 CLSRTAKQLR DSGLPARLTE EQLHRIWRGG PKKCDG
	Isoform nsp1a. See Q4TWH9.	FASTA	–	–

References

[1]

"Molecular characterization of human astroviruses isolated in Brazil, including the complete sequences of astrovirus genotypes 4 and 5." Silva P.A., Cardoso D.D., Schreier E. Arch. Virol. 151:1405-1417(2006) [PubMed: 16421636] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Goiania/GO/12/94/Brazil.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ028633 Genomic RNA. Translation: AAY46273.1. Sequence problems.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR001205. RNA_pol_P3D. IPR007094. RNA_pol_PSvir. [Graphical view]
Pfam	PF00680. RdRP_1. 1 hit. [Graphical view]
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NS1AB_HASV5

Accession

Primary (citable) accession number: Q4TWH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	April 8, 2008
Last modified:	June 16, 2009
This is version 22 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)