Reviewed, UniProtKB/Swiss-Prot Q4R945 (TTK_MACFA)

Names and origin

Protein names

Recommended name:
    Dual specificity protein kinase TTK
    EC=2.7.12.1

Gene names

Name:	TTK
ORF Names:	QtsA-10742

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	856 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Inhibited by the ATP-competitive kinase inhibitor, SP600125 By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 856	856	Dual specificity protein kinase TTK		PRO_0000311351
Regions
	Domain	524 – 790	267	Protein kinase
	Nucleotide binding	530 – 538	9	ATP By similarity
Sites
	Active site	646	1	Proton acceptor By similarity
	Binding site	552	1	ATP By similarity
Amino acid modifications
	Modified residue	7	1	Phosphoserine By similarity
	Modified residue	33	1	Phosphothreonine By similarity
	Modified residue	37	1	Phosphoserine By similarity
	Modified residue	80	1	Phosphoserine By similarity
	Modified residue	281	1	Phosphoserine By similarity
	Modified residue	317	1	Phosphoserine By similarity
	Modified residue	321	1	Phosphoserine By similarity
	Modified residue	393	1	Phosphoserine By similarity
	Modified residue	435	1	Phosphoserine By similarity
	Modified residue	820	1	Phosphoserine By similarity
	Modified residue	823	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4R945-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 194168C4A238E22B	FASTA	856	96,783
	10 20 30 40 50 60 MESEDLSGRE LTIDSIMNKV RDIKNKFKNE DLTDELSLNK ISADTTDNSG TVNQIMMMAN 70 80 90 100 110 120 SPEDWLSLLL KLEKNSVPLS DALLNKLIGR YSQAIEALPP DKYGQNESFA RIQVRFAELK 130 140 150 160 170 180 AIQEPDDARD YFQMARANCK KFAFVHISFA QFELSQGNVK KSKQLLQEAV ERGAVPLEML 190 200 210 220 230 240 EIALRNLNLQ KKQLLSEEEK KTLSASMVLT AQESFSSSLG HLQNRNNSCD SRGQTTKARF 250 260 270 280 290 300 LYGENMPPQD AEIGYRNSLK QTNKTKQSCP FGRVPVNLLN SPDCDVKTDD SVVPCFIKRQ 310 320 330 340 350 360 TSRSECRDLV VAGSKPSGND SCELRNLKSV QNINCKEPLV SDEKSSELII TDSVTLKNKT 370 380 390 400 410 420 ESSLRAKLEE TKEYQEPEVP ESNQKQWQSK RKSACINENP VASSNQWQIP ELARKVNIEK 430 440 450 460 470 480 HTTFEQPVFS VSKQSPPMSA SKWFDPKSIC KTPSSNTLDD YMSCFRTPVV KNDFPPACQL 490 500 510 520 530 540 STPYGQPACF QQQQQQIPAT PLQNLQVLAS SSANECISVK GRIYSILKQI GSGGSSKVFQ 550 560 570 580 590 600 VLNEKKQIYA IKFVNLEEAD NQTLDSYRNE IAYLNKLQQH SDKIIRLYDY EITDQYIYMV 610 620 630 640 650 660 MECGNIDLNS WLKKKKSIDP WERKSYWKNM LEAVHTIHQH GIVHSDLKPA NFLIVDGMLK 670 680 690 700 710 720 LIDFGIANQM QPDTTSIVKD SQVGTVNYMP PEAIKDMSSS RENGKSKSKI SPKSDVWSLG 730 740 750 760 770 780 CILYYMTYGK TPFQHIINQI SKLHAIIDPN HEIEFPDIPE KDLQDVLKCC LKRDPKQRIS 790 800 810 820 830 840 IPELLAHPYV QIQTHPGNQM AKGTTEEMKY VLGQLVGLNS PNSILKAAKT LYEHYSGGES 850 HNSSSSKTFG KKREKK

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB168252 mRNA. Translation: BAE00376.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q4R945.
Enzyme and pathway databases
BRENDA	2.7.12.1. 3438.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TTK_MACFA

Accession

Primary (citable) accession number: Q4R945

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	July 19, 2005
Last modified:	October 13, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)