Reviewed, UniProtKB/Swiss-Prot Q4R8S6 (CP51A_MACFA)

Names and origin

Protein names

Recommended name:
    Lanosterol 14-alpha demethylase
      Short name=LDM
    EC=1.14.13.70
Alternative name(s):
    Cytochrome P450 51A1
    CYPLI
    Cytochrome P450LI
    Sterol 14-alpha demethylase
    Cytochrome P450-14DM
      Short name=Cytochrome P45014DM

Gene names

Name:	CYP51A1
Synonyms:	CYP51
ORF Names:	QtsA-11582, QtsA-20162

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol By similarity.
Catalytic activity	Obtusifoliol + 3 O2 + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3 NADP+ + 4 H2O.
Cofactor	Heme group By similarity.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.
Subcellular location	Endoplasmic reticulum membrane By similarity. Microsome membrane By similarity.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sterol 14-demethylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Lanosterol 14-alpha demethylase		PRO_0000052000
Regions
	Transmembrane	24 – 44	21	Potential
Sites
	Metal binding	449	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4R8S6-1 [UniParc]. Last modified May 26, 2009. Version 2. Checksum: DEC563E20B0666DB	FASTA	503	56,815
	10 20 30 40 50 60 MMLLGLLQAG GSVLGQAMEK VTGGNLLSML LIACAFTLSL VYLFRLAAGH LVQLPAGAKS 70 80 90 100 110 120 PPYIFSPIPF LGHAIAFGKS PVEFLENAYE KYGPVFSFTM VGKTFTYLLG SDAAALLFNS 130 140 150 160 170 180 KNEDLNAEDV YSRLTTPVFG KGVAYDVPNP VFLEQKKMLK SGLNIAHFKQ HVSIIEKETK 190 200 210 220 230 240 EYFQSWGESG EKNVFEALSE LIILTASHCL HGKEIRSQLN EKVAQLYADL DGGFSHAAWL 250 260 270 280 290 300 LPGWLPLPSF RRRDRAHREI KNIFYKAIQK RRQSQEKIDD ILQTLLDATY KDGRPLTDDE 310 320 330 340 350 360 VAGMLIGLLL AGQHTSSTTS AWMGFFLARD KTLQEKCYLE QKTVCGENLP PLTYDQLKDL 370 380 390 400 410 420 NLLDRCIKET LRLRPPIMIM MRMARTPQTV AGYTIPPGHQ VCVSPTVNQR LKDSWVERLD 430 440 450 460 470 480 FNPDRYLQDN PASGEKFAYV PFGAGRHRCI GENFAYVQIK TIWSTMLRLY EFDLIDGYFP 490 500 TVNYTTMIHT PENPVIRYKR RSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of CYP cDNAs from cynomolgus monkey liver." Uno Y., Kito G., Kamataki T., Nagata R. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ074804 mRNA. Translation: AAZ29450.1. Different initiation. AB168372 mRNA. Translation: BAE00495.1. Different initiation. AB169425 mRNA. Translation: BAE01508.1. Different initiation.
3D structure databases
HSSP	HSSP built from PDB template 1X8V based on UniProtKB P0A512.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q4R8S6.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP51A_MACFA

Accession

Primary (citable) accession number: Q4R8S6
Secondary accession number(s): Q4R5W7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	May 26, 2009
Last modified:	January 19, 2010
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)