Q4R8S6 (CP51A_MACFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lanosterol 14-alpha demethylase
Short name=LDM
EC=1.14.13.70

Alternative name(s):
CYPLI
Cytochrome P450 51A1
Cytochrome P450-14DM
Short name=Cytochrome P45014DM
Cytochrome P450LI
Sterol 14-alpha demethylase

Gene names

Name:	CYP51A1
Synonyms:	CYP51
ORF Names:	QtsA-11582, QtsA-20162

Organism

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Cercopithecoidea › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol By similarity.
Catalytic activity	A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.
Cofactor	Heme group By similarity.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.
Subcellular location	Endoplasmic reticulum membrane By similarity. Microsome membrane By similarity.
Sequence similarities	Belongs to the cytochrome P450 family.
Sequence caution	The sequence AAZ29450.1 differs from that shown. Reason: Erroneous initiation. The sequence BAE00495.1 differs from that shown. Reason: Erroneous initiation. The sequence BAE01508.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Cholesterol metabolism Lipid biosynthesis Lipid metabolism Steroid biosynthesis Steroid metabolism Sterol biosynthesis Sterol metabolism
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological_process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro sterol 14-demethylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Lanosterol 14-alpha demethylase		PRO_0000052000
Regions
	Transmembrane	24 – 44	21	Helical; Potential
Sites
	Metal binding	449	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4R8S6 [UniParc]. Last modified May 26, 2009. Version 2. Checksum: DEC563E20B0666DB	FASTA	503	56,815
	10 20 30 40 50 60 MMLLGLLQAG GSVLGQAMEK VTGGNLLSML LIACAFTLSL VYLFRLAAGH LVQLPAGAKS 70 80 90 100 110 120 PPYIFSPIPF LGHAIAFGKS PVEFLENAYE KYGPVFSFTM VGKTFTYLLG SDAAALLFNS 130 140 150 160 170 180 KNEDLNAEDV YSRLTTPVFG KGVAYDVPNP VFLEQKKMLK SGLNIAHFKQ HVSIIEKETK 190 200 210 220 230 240 EYFQSWGESG EKNVFEALSE LIILTASHCL HGKEIRSQLN EKVAQLYADL DGGFSHAAWL 250 260 270 280 290 300 LPGWLPLPSF RRRDRAHREI KNIFYKAIQK RRQSQEKIDD ILQTLLDATY KDGRPLTDDE 310 320 330 340 350 360 VAGMLIGLLL AGQHTSSTTS AWMGFFLARD KTLQEKCYLE QKTVCGENLP PLTYDQLKDL 370 380 390 400 410 420 NLLDRCIKET LRLRPPIMIM MRMARTPQTV AGYTIPPGHQ VCVSPTVNQR LKDSWVERLD 430 440 450 460 470 480 FNPDRYLQDN PASGEKFAYV PFGAGRHRCI GENFAYVQIK TIWSTMLRLY EFDLIDGYFP 490 500 TVNYTTMIHT PENPVIRYKR RSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of CYP cDNAs from cynomolgus monkey liver." Uno Y., Kito G., Kamataki T., Nagata R. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ074804 mRNA. Translation: AAZ29450.1. Different initiation. AB168372 mRNA. Translation: BAE00495.1. Different initiation. AB169425 mRNA. Translation: BAE01508.1. Different initiation.
3D structure databases
HSSP	HSSP built from PDB template 1X8V based on UniProtKB P0A512.
ModBase	Search...
Proteomic databases
PRIDE	Q4R8S6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00770; UER00754.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP51A_MACFA

Accession

Primary (citable) accession number: Q4R8S6
Secondary accession number(s): Q4R5W7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	May 26, 2009
Last modified:	May 1, 2013
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program