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Q4R8S6 (CP51A_MACFA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lanosterol 14-alpha demethylase

Short name=LDM
EC=1.14.13.70
Alternative name(s):
CYPLI
Cytochrome P450 51A1
Cytochrome P450-14DM
Short name=Cytochrome P45014DM
Cytochrome P450LI
Sterol 14-alpha demethylase
Gene names
Name:CYP51A1
Synonyms:CYP51
ORF Names:QtsA-11582, QtsA-20162
OrganismMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifier9541 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol By similarity.

Catalytic activity

A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.

Cofactor

Heme group By similarity.

Pathway

Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.

Subcellular location

Endoplasmic reticulum membrane By similarity. Microsome membrane By similarity.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence caution

The sequence AAZ29450.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAE00495.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAE01508.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 503503Lanosterol 14-alpha demethylase
PRO_0000052000

Regions

Transmembrane24 – 4421Helical; Potential

Sites

Metal binding4491Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4R8S6 [UniParc].

Last modified May 26, 2009. Version 2.
Checksum: DEC563E20B0666DB

FASTA50356,815
        10         20         30         40         50         60 
MMLLGLLQAG GSVLGQAMEK VTGGNLLSML LIACAFTLSL VYLFRLAAGH LVQLPAGAKS 

        70         80         90        100        110        120 
PPYIFSPIPF LGHAIAFGKS PVEFLENAYE KYGPVFSFTM VGKTFTYLLG SDAAALLFNS 

       130        140        150        160        170        180 
KNEDLNAEDV YSRLTTPVFG KGVAYDVPNP VFLEQKKMLK SGLNIAHFKQ HVSIIEKETK 

       190        200        210        220        230        240 
EYFQSWGESG EKNVFEALSE LIILTASHCL HGKEIRSQLN EKVAQLYADL DGGFSHAAWL 

       250        260        270        280        290        300 
LPGWLPLPSF RRRDRAHREI KNIFYKAIQK RRQSQEKIDD ILQTLLDATY KDGRPLTDDE 

       310        320        330        340        350        360 
VAGMLIGLLL AGQHTSSTTS AWMGFFLARD KTLQEKCYLE QKTVCGENLP PLTYDQLKDL 

       370        380        390        400        410        420 
NLLDRCIKET LRLRPPIMIM MRMARTPQTV AGYTIPPGHQ VCVSPTVNQR LKDSWVERLD 

       430        440        450        460        470        480 
FNPDRYLQDN PASGEKFAYV PFGAGRHRCI GENFAYVQIK TIWSTMLRLY EFDLIDGYFP 

       490        500 
TVNYTTMIHT PENPVIRYKR RSK 

« Hide

References

« Hide 'large scale' references
[1]"Identification of CYP cDNAs from cynomolgus monkey liver."
Uno Y., Kito G., Kamataki T., Nagata R.
Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
International consortium for macaque cDNA sequencing and analysis
Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ074804 mRNA. Translation: AAZ29450.1. Different initiation.
AB168372 mRNA. Translation: BAE00495.1. Different initiation.
AB169425 mRNA. Translation: BAE01508.1. Different initiation.
RefSeqNP_001274625.1. NM_001287696.1.
UniGeneMfa.1856.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ4R8S6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID102146764.
KEGGmcf:102146764.

Phylogenomic databases

KOK05917.

Enzyme and pathway databases

UniPathwayUPA00770; UER00754.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00465. EP450IV.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP51A_MACFA
AccessionPrimary (citable) accession number: Q4R8S6
Secondary accession number(s): Q4R5W7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: May 26, 2009
Last modified: June 11, 2014
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways