Reviewed, UniProtKB/Swiss-Prot Q4R8B6 (F264_MACFA)

Names and origin

Protein names

Recommended name:
    6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
Alternative name(s):
    6PF-2-K/Fru-2,6-P2ASE testis-type isozyme
Including the following 2 domains:
    1- Recommended name:
            6-phosphofructo-2-kinase
              EC=2.7.1.105
    2- Recommended name:
            Fructose-2,6-bisphosphatase
              EC=3.1.3.46

Gene names

Name:	PFKFB4
ORF Names:	QtsA-12874

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Synthesis and degradation of fructose 2,6-bisphosphate By similarity.
Catalytic activity	Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.
Enzyme regulation	The most important regulatory mechanism of these opposing activities is by phosphorylation and dephosphorylation of the enzyme By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	In the C-terminal section; belongs to the phosphoglycerate mutase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Transferase
PTM	Phosphoprotein
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	fructose 2,6-bisphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructo-2-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructose-2,6-bisphosphate 2-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 469	468	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4		PRO_0000268186
Regions
	Nucleotide binding	46 – 53	8	ATP Potential
	Region	2 – 249	248	6-phosphofructo-2-kinase
	Region	250 – 469	220	Fructose-2,6-bisphosphatase
Sites
	Active site	129	1	Potential
	Active site	159	1	Potential
	Active site	257	1	Tele-phosphohistidine intermediate By similarity
	Active site	326	1	Potential
	Active site	391	1	Proton donor By similarity
	Binding site	103	1	Fructose-6-phosphate By similarity
	Binding site	194	1	Fructose-6-phosphate By similarity
Amino acid modifications
	Modified residue	444	1	Phosphothreonine; by PKC Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4R8B6-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 0ABB1148962456D8	FASTA	469	54,071
	10 20 30 40 50 60 MASPRELTQN PLKKIWMPYS NGRPALHACQ RGVCMTNCPT LIVMVGLPAR GKTYISKKLT 70 80 90 100 110 120 RYLNWIGVPT REFNVGQYRR NMVKTYKSFE FFLPDNEEGL KIRKQCALAA LRDVRRFLSE 130 140 150 160 170 180 EGGHVAVFDA TNTTRERRAT IFNFGEQNGY KTFFVESICV DPEVIAANIV QVKLGSPDYV 190 200 210 220 230 240 NRDSDEATED FMRRIECYEN SYESLDEDLD RDLSYIKIMD VGQSYVVNRV ADHIQSRIVY 250 260 270 280 290 300 YLMNIHVTPR SIYLCRHGES ELNLKGRIGG DPGLSPRGRE FAKSLAQFIS DQNIKDLKVW 310 320 330 340 350 360 TSQMKRTIQT AEALGVPYEQ WKVLNEIDAG VCEEMTYEEI QDNYPLEFAL RDQDKYRYRY 370 380 390 400 410 420 PKGESYEDLV QRLEPVIMEL ERQENVLVIC HQAVMRCLLA YFLDKAAEQL PYLKCPLHTV 430 440 450 460 LKLTPVAYGC KVESIFLNVA AVNTHRDRPQ NVDISRPPEE ALVTVPAHQ

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB168541 mRNA. Translation: BAE00656.1.
3D structure databases
SMR	Q4R8B6. Positions 38-469.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q4R8B6.
Enzyme and pathway databases
BRENDA	2.7.1.105. 3438. 3.1.3.46. 3438.
Family and domain databases
InterPro	IPR003094. 6Pfruct_kin. IPR013079. 6Phosfructo_kin. IPR016260. Bifunct_6PFK/fruc_bisP_Ptase. IPR001345. PG/BPGM_mutase. IPR013078. PG_mutase. [Graphical view]
PANTHER	PTHR10606. 6Pfruct_kin. 1 hit.
Pfam	PF01591. 6PF2K. 1 hit. PF00300. PGAM. 1 hit. [Graphical view]
PIRSF	PIRSF000709. 6PFK_2-Ptase. 1 hit.
PRINTS	PR00991. 6PFRUCTKNASE.
PROSITE	PS00175. PG_MUTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F264_MACFA

Accession

Primary (citable) accession number: Q4R8B6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 24 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)