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Protein

Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A

Gene

MGAT4A

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Cofactori

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT54. Glycosyltransferase Family 54.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A (EC:2.4.1.145)
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa
Short name:
GlcNAc-T IVa
Short name:
GnT-IVa
Short name:
N-acetylglucosaminyltransferase IVa
UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVa
Cleaved into the following chain:
Gene namesi
Name:MGAT4A
ORF Names:QtsA-13423
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 44CytoplasmicSequence analysis
Transmembranei5 – 2723Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini28 – 535508LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 535535Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase APRO_0000288583Add
BLAST
Chaini93 – 535443Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble formBy similarityPRO_0000288584Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi77 – 771N-linked (GlcNAc...)Sequence analysis
Glycosylationi458 – 4581N-linked (GlcNAc...)Sequence analysis
Modified residuei474 – 4741PhosphoserineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili28 – 6336Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyltransferase 54 family.Curated

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG053194.
KOiK00738.

Family and domain databases

InterProiIPR006759. Glyco_transf_54.
[Graphical view]
PANTHERiPTHR12062. PTHR12062. 1 hit.
PfamiPF04666. Glyco_transf_54. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4R854-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLRNGTVAT ALAFITSFLT LSWYTTWQNG KEKLIAYQRE FLALKERLRI
60 70 80 90 100
AEHRISQRSS ELNTIVQQFK RVGAETNGSK DALNKFSDNT LKLLKELTSK
110 120 130 140 150
KSLQVPSIYY HLPHLLKNEG SLRPAVQIGN GRTGVSIVMG IPTVKREVKS
160 170 180 190 200
YLIETLHSLI DNLYPEEKLD CVIVVFIGET DTDYVHGVVA NLEKEFSKEI
210 220 230 240 250
SSGLVEVISP PESYYPDLTN LKETFGDSKE RVRWRTKQNL DYCFLMMYAQ
260 270 280 290 300
EKGIYYIQLE DDIIVKQNYF NTIKNFALQL SSEEWMILEF SQLGFIGKMF
310 320 330 340 350
QAPDLTLIVE FIFMFYKEKP IDWLLDHILW VKVCNPEKDA KHCDRQKANL
360 370 380 390 400
RIRFRPSLFQ HVGLHSSLSG KIQKLTDKDY MKPLLLKIHV NPPAEVSTSL
410 420 430 440 450
KVYQGHTLEK TYMGEDFFWA ITPIAGDYIL FKFDKPVNVE SYLFHSGNQE
460 470 480 490 500
HPGDILLNTT VEVLPFKSEG LEISKETKDK RLEDGYFRIG KFENGVAEGM
510 520 530
VDPSLNPISA FRLSVIQNSA VWAILNEIHI KKATK
Length:535
Mass (Da):61,574
Last modified:July 19, 2005 - v1
Checksum:iB0FA05A1B9B04E29
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB168604 mRNA. Translation: BAE00718.1.
RefSeqiNP_001271500.1. NM_001284571.1.
XP_005575110.1. XM_005575053.2.
UniGeneiMfa.76.

Genome annotation databases

GeneIDi101866133.
KEGGimcf:101866133.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB168604 mRNA. Translation: BAE00718.1.
RefSeqiNP_001271500.1. NM_001284571.1.
XP_005575110.1. XM_005575053.2.
UniGeneiMfa.76.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT54. Glycosyltransferase Family 54.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi101866133.
KEGGimcf:101866133.

Organism-specific databases

CTDi11320.

Phylogenomic databases

HOVERGENiHBG053194.
KOiK00738.

Enzyme and pathway databases

UniPathwayiUPA00378.

Family and domain databases

InterProiIPR006759. Glyco_transf_54.
[Graphical view]
PANTHERiPTHR12062. PTHR12062. 1 hit.
PfamiPF04666. Glyco_transf_54. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMGT4A_MACFA
AccessioniPrimary (citable) accession number: Q4R854
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: July 19, 2005
Last modified: April 13, 2016
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.