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Protein

Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A

Gene

MGAT4A

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-(beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6))-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-(beta-D-GlcNAc-(1->4))-alpha-D-Man-(1->3)-(beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6))-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein].

Cofactori

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandMetal-binding

Enzyme and pathway databases

UniPathwayiUPA00378

Protein family/group databases

CAZyiGT54 Glycosyltransferase Family 54

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A (EC:2.4.1.145)
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa
Short name:
GlcNAc-T IVa
Short name:
GnT-IVa
Short name:
N-acetylglucosaminyltransferase IVa
UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVa
Cleaved into the following chain:
Gene namesi
Name:MGAT4A
ORF Names:QtsA-13423
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4CytoplasmicSequence analysis4
Transmembranei5 – 27Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST23
Topological domaini28 – 535LumenalSequence analysisAdd BLAST508

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002885831 – 535Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase AAdd BLAST535
ChainiPRO_000028858493 – 535Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble formBy similarityAdd BLAST443

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi77N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi458N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei474PhosphoserineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili28 – 63Sequence analysisAdd BLAST36

Sequence similaritiesi

Belongs to the glycosyltransferase 54 family.Curated

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000074578
HOVERGENiHBG053194
KOiK00738

Family and domain databases

InterProiView protein in InterPro
IPR006759 Glyco_transf_54
PANTHERiPTHR12062 PTHR12062, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4R854-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLRNGTVAT ALAFITSFLT LSWYTTWQNG KEKLIAYQRE FLALKERLRI
60 70 80 90 100
AEHRISQRSS ELNTIVQQFK RVGAETNGSK DALNKFSDNT LKLLKELTSK
110 120 130 140 150
KSLQVPSIYY HLPHLLKNEG SLRPAVQIGN GRTGVSIVMG IPTVKREVKS
160 170 180 190 200
YLIETLHSLI DNLYPEEKLD CVIVVFIGET DTDYVHGVVA NLEKEFSKEI
210 220 230 240 250
SSGLVEVISP PESYYPDLTN LKETFGDSKE RVRWRTKQNL DYCFLMMYAQ
260 270 280 290 300
EKGIYYIQLE DDIIVKQNYF NTIKNFALQL SSEEWMILEF SQLGFIGKMF
310 320 330 340 350
QAPDLTLIVE FIFMFYKEKP IDWLLDHILW VKVCNPEKDA KHCDRQKANL
360 370 380 390 400
RIRFRPSLFQ HVGLHSSLSG KIQKLTDKDY MKPLLLKIHV NPPAEVSTSL
410 420 430 440 450
KVYQGHTLEK TYMGEDFFWA ITPIAGDYIL FKFDKPVNVE SYLFHSGNQE
460 470 480 490 500
HPGDILLNTT VEVLPFKSEG LEISKETKDK RLEDGYFRIG KFENGVAEGM
510 520 530
VDPSLNPISA FRLSVIQNSA VWAILNEIHI KKATK
Length:535
Mass (Da):61,574
Last modified:July 19, 2005 - v1
Checksum:iB0FA05A1B9B04E29
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB168604 mRNA Translation: BAE00718.1
RefSeqiNP_001271500.1, NM_001284571.1
XP_005575110.1, XM_005575053.2
UniGeneiMfa.76

Genome annotation databases

EnsembliENSMFAT00000033176; ENSMFAP00000025029; ENSMFAG00000044000
GeneIDi101866133
KEGGimcf:101866133

Entry informationi

Entry nameiMGT4A_MACFA
AccessioniPrimary (citable) accession number: Q4R854
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: July 19, 2005
Last modified: May 23, 2018
This is version 52 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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