Reviewed, UniProtKB/Swiss-Prot Q4R802 (AKCL2_MACFA)

Names and origin

Protein names

Recommended name:
    1,5-anhydro-D-fructose reductase
      Short name=AF reductase
    EC=1.1.1.263
Alternative name(s):
    Aldo-keto reductase family 1 member C-like protein 2

Gene names

Name:	AKR1CL2
ORF Names:	QtsA-13895

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 1,5-anhydro-D-fructose (AF) to 1,5-anhydro-D-glucitol. Can also catalyze the reduction of various aldehydes and quinones By similarity.
Catalytic activity	1,5-anhydro-D-glucitol + NADP+ = 1,5-anhydro-D-fructose + NADPH.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	1,5-anhydro-D-fructose reductase		PRO_0000287881
Sites
	Active site	40	1	Proton donor By similarity
	Binding site	102	1	Substrate By similarity
	Site	69	1	Lowers pKa of active site Tyr By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4R802-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 5D7D2F96E3FBD662	FASTA	320	36,418
	10 20 30 40 50 60 MGDIPAVGLS CWKASPGKVT EAVKVAIDAG YRHFNCAYFY HNEKEVGAGI RYKIKEGAVR 70 80 90 100 110 120 REDLFIASKL WCTCHKKSLV KTACRRSLKA LKLNYLDLYL IHWPMGFKPP HPEWIMSCSE 130 140 150 160 170 180 LSFCLSHPGV HDLPLDESDM VIPGDTDFLD TWEAMEDLVI TGLVKNIGVS NFNHEQLERL 190 200 210 220 230 240 LNKPGLRFKP VTNQIECHPY LTQKNLISFC QSRGVSVTAY RPLGGSCEGV DLIDDPVIQR 250 260 270 280 290 300 IAKEHSKSPA QILIRFQTQR NVIVIPGSIT PSHIKENIQV FDFELTQHDM DNILSLDRNL 310 320 RLATFPITKN HKDYPFHIEY

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB168659 mRNA. Translation: BAE00770.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q4R802.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. False negative. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AKCL2_MACFA

Accession

Primary (citable) accession number: Q4R802

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	July 19, 2005
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)