Reviewed, UniProtKB/Swiss-Prot Q4R7L8 (NUD12_MACFA)

Names and origin

Protein names

Recommended name:
    Peroxisomal NADH pyrophosphatase NUDT12
    EC=3.6.1.22
Alternative name(s):
    Nucleoside diphosphate-linked moiety X motif 12
      Short name=Nudix motif 12

Gene names

Name:	NUDT12
ORF Names:	QtsA-14876

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes NAD(P)H to NMNH and AMP (2',5'-ADP), and diadenosine diphosphate to AMP. Has also activity towards NAD(P)+, ADP-ribose and diadenosine triphosphate. May act to regulate the concentration of peroxisomal nicotinamide nucleotide cofactors required for oxidative metabolism in this organelle By similarity.
Catalytic activity	NAD+ + H2O = AMP + NMN.
Cofactor	Magnesium or manganese By similarity.
Subcellular location	Peroxisome By similarity.
Sequence similarities	Belongs to the Nudix hydrolase family. NudC subfamily. Contains 3 ANK repeats.

Ontologies

Keywords
Cellular component	Peroxisome
Domain	ANK repeat Repeat
Ligand	Magnesium Manganese Metal-binding NAD NADP
Molecular function	Hydrolase
Gene Ontology (GO)
Cellular component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD+ diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	Peroxisomal NADH pyrophosphatase NUDT12		PRO_0000260766
Regions
	Repeat	11 – 40	30	ANK 1
	Repeat	45 – 74	30	ANK 2
	Repeat	78 – 98	21	ANK 3
	Motif	355 – 376	22	Nudix box
	Motif	460 – 462	3	Microbody targeting signal By similarity
Sites
	Metal binding	370	1	Magnesium or manganese By similarity
	Metal binding	374	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4R7L8-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: BD4B6171E80DCB25	FASTA	462	52,092
	10 20 30 40 50 60 MSSVKRSPKQ EIVTQFHCSA AEGDIAKLTG ILSHSPSLLN ETSENGWTAL MYAARNGHPE 70 80 90 100 110 120 IVQFLLEKGC DRSIVNKSRQ TALDIAVFWG YKHIANLLAT AKGGKKPWFL TNEVEECENY 130 140 150 160 170 180 FSKTLLDRKS EKRNNADWLL AKESHPATVF ILFSDLNPLV TLGGNKESFQ QPEVRLCQLN 190 200 210 220 230 240 YKDIKDYLAQ PEEITLIFLG VELEMKDKLL NYAGEVPREE EDGLVAWFAL GIDPIAAEEF 250 260 270 280 290 300 KQRHENCYFL HPPMPALLQL KEKEAGVVAQ ARSVLAWHSR YKFCPTCGNG TKIEEGGYKR 310 320 330 340 350 360 VCLKEDCPSL NGVHNTSYPR VDPVVIMQVI HPDGTRCLLG RQKRFPPGMF TCLAGFIEPG 370 380 390 400 410 420 ETIEDAVRRE VEEESGVKVG HVQYVSCQPW PMPSSLMIGC LAVAVSTEIK VDKNEIEDAR 430 440 450 460 WFTREQVLDV LTKGKQQAFF VPPSRAIAHQ LIKHWIRINP NL

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB168797 mRNA. Translation: BAE00904.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q4R7L8.
Enzyme and pathway databases
BRENDA	3.6.1.22. 3438.
Family and domain databases
InterPro	IPR002110. ANK. IPR015375. NADH_PPase-like_N. IPR000086. NUDIX_hydrolase_core. IPR015376. Znr_NADH_PPase. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 1 hit. G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
Pfam	PF00023. Ank. 2 hits. PF00293. NUDIX. 1 hit. PF09296. NUDIX-like. 1 hit. PF09297. zf-NADH-PPase. 1 hit. [Graphical view]
SMART	SM00248. ANK. 3 hits. [Graphical view]
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 1 hit. PS00893. NUDIX. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUD12_MACFA

Accession

Primary (citable) accession number: Q4R7L8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	July 19, 2005
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)