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Q4R7K1 (MCES_MACFA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
mRNA cap guanine-N7 methyltransferase
EC=2.1.1.56
Alternative name(s):
RG7MT1
mRNA (guanine-N(7)-)-methyltransferase
mRNA cap methyltransferase
Gene names
Name:RNMT
ORF Names:QtsA-15044
OrganismMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifier9541 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length476 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC By similarity.

Catalytic activity

S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

Subunit structure

Interacts with importin alpha, leading to stimulate both RNA-binding and methyltransferase activity. Interaction with importin alpha and beta is required for its nuclear localization, importin beta dissociating in response to RanGTP, allowing RNMT-importin alpha to bind RNA substrates. Interacts with elongating form of polymerase II and RNGTT. Interacts with RAM/FAM103A1, the interaction significantly enhances RNA-binding and cap methyltransferase activity By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the methyltransferase superfamily. mRNA cap methyltransferase family.

Ontologies

Keywords
   Biological processmRNA capping
mRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   PTMPhosphoprotein
Gene Ontology (GO)
   Biological processmRNA capping

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular componentnucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular functionRNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

mRNA (guanine-N7-)-methyltransferase activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 476476mRNA cap guanine-N7 methyltransferase
PRO_0000248322

Regions

Region176 – 1772mRNA cap binding By similarity
Motif126 – 1283Nuclear localization signal By similarity

Sites

Binding site1801S-adenosyl-L-methionine By similarity
Binding site2051S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site2081mRNA cap By similarity
Binding site2141mRNA cap By similarity
Binding site2271S-adenosyl-L-methionine By similarity
Binding site2391mRNA cap By similarity
Binding site2611S-adenosyl-L-methionine By similarity
Binding site2841S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site2881mRNA cap By similarity
Binding site3701mRNA cap By similarity
Binding site4671mRNA cap By similarity

Amino acid modifications

Modified residue241Phosphoserine By similarity
Modified residue281Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4R7K1 [UniParc].

Last modified July 19, 2005. Version 1.
Checksum: D22D4B4CE5A3A1E0

FASTA47654,797
        10         20         30         40         50         60 
MANSTKAEEY EKMSVEQAKA SVNSEAESSF SINENTTASG TGLSGKTSVC RQVDTARKRK 

        70         80         90        100        110        120 
EFEDDLVKES SSCGEGTPSK KRKLDPEIVP EEKDCGDDEG NSKKRKRETE DVPKDEYSTG 

       130        140        150        160        170        180 
DGTQNKRKIA LEDVPEKQKN LEEGHSSAVA AHYNELQEVG LEKRSQSRIF YLRNFNNWMK 

       190        200        210        220        230        240 
SVLIGEFLEK VRQKKKRDIT VLDLGCGKGG DLLKWKKGRI NKLVCTDIAD VSIKQCQQRY 

       250        260        270        280        290        300 
EDMKNRRDSE YIFSAEFITA DCSKELLIEK FRDPQMCFDI CSCQFVCHYS FESYEQADMM 

       310        320        330        340        350        360 
LRNACERLSP GGYFIGTTPN SFELIRRLEA SETESFGNEI YTVKFQKKGD YPLFGCKYDF 

       370        380        390        400        410        420 
NLEGVVDVPE FLVYFPLLNE MAKKYNMKLV YKKTFLEFYE EKIKNNENKM LLKRMQALEP 

       430        440        450        460        470 
YPANESSKLV SERVDDYEHA AKYMKNSQVK LPLGTLSKSE WEATSIYLVF AFEKQQ

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References

[1]"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
International consortium for macaque cDNA sequencing and analysis
Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB168817 mRNA. Translation: BAE00921.1.

3D structure databases

HSSPHSSP built from PDB template 1RI5 based on UniProtKB Q8SR66.
ProteinModelPortalQ4R7K1.
SMRQ4R7K1. Positions 169-476.
ModBaseSearch...

Proteomic databases

PRIDEQ4R7K1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG081963.

Family and domain databases

InterProIPR016899. mRNA_G-N7_MeTrfase.
IPR004971. Pox_MCEL.
[Graphical view]
PfamPF03291. Pox_MCEL. 1 hit.
[Graphical view]
PIRSFPIRSF028762. ABD1. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMCES_MACFA
AccessionPrimary (citable) accession number: Q4R7K1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: July 19, 2005
Last modified: January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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