Reviewed, UniProtKB/Swiss-Prot Q4R7K1 (MCES_MACFA)

Names and origin

Protein names

Recommended name:
    mRNA cap guanine-N7 methyltransferase
    EC=2.1.1.56
Alternative name(s):
    mRNA (guanine-N(7)-)-methyltransferase
    RG7MT1
    mRNA cap methyltransferase

Gene names

Name:	RNMT
ORF Names:	QtsA-15044

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC By similarity.
Catalytic activity	S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with importin alpha, leading to stimulate both RNA-binding and methyltransferase activity. Interaction with importin alpha and beta is required for its nuclear localization, importin beta dissociating in response to RanGTP, allowing RNMT-importin alpha to bind RNA substrates. Interacts with elongating form of polymerase II and RNGTT By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the mRNA cap methyltransferase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	mRNA capping Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	RNA binding Inferred from sequence or structural similarity. Source: UniProtKB mRNA (guanine-N7-)-methyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	mRNA cap guanine-N7 methyltransferase		PRO_0000248322
Regions
	Region	176 – 177	2	mRNA cap binding By similarity
	Motif	126 – 128	3	Nuclear localization signal By similarity
Sites
	Binding site	180	1	S-adenosyl-L-methionine By similarity
	Binding site	205	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	208	1	mRNA cap By similarity
	Binding site	214	1	mRNA cap By similarity
	Binding site	227	1	S-adenosyl-L-methionine By similarity
	Binding site	239	1	mRNA cap By similarity
	Binding site	261	1	S-adenosyl-L-methionine By similarity
	Binding site	284	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	288	1	mRNA cap By similarity
	Binding site	370	1	mRNA cap By similarity
	Binding site	467	1	mRNA cap By similarity
Amino acid modifications
	Modified residue	24	1	Phosphoserine By similarity
	Modified residue	28	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4R7K1-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: D22D4B4CE5A3A1E0	FASTA	476	54,797
	10 20 30 40 50 60 MANSTKAEEY EKMSVEQAKA SVNSEAESSF SINENTTASG TGLSGKTSVC RQVDTARKRK 70 80 90 100 110 120 EFEDDLVKES SSCGEGTPSK KRKLDPEIVP EEKDCGDDEG NSKKRKRETE DVPKDEYSTG 130 140 150 160 170 180 DGTQNKRKIA LEDVPEKQKN LEEGHSSAVA AHYNELQEVG LEKRSQSRIF YLRNFNNWMK 190 200 210 220 230 240 SVLIGEFLEK VRQKKKRDIT VLDLGCGKGG DLLKWKKGRI NKLVCTDIAD VSIKQCQQRY 250 260 270 280 290 300 EDMKNRRDSE YIFSAEFITA DCSKELLIEK FRDPQMCFDI CSCQFVCHYS FESYEQADMM 310 320 330 340 350 360 LRNACERLSP GGYFIGTTPN SFELIRRLEA SETESFGNEI YTVKFQKKGD YPLFGCKYDF 370 380 390 400 410 420 NLEGVVDVPE FLVYFPLLNE MAKKYNMKLV YKKTFLEFYE EKIKNNENKM LLKRMQALEP 430 440 450 460 470 YPANESSKLV SERVDDYEHA AKYMKNSQVK LPLGTLSKSE WEATSIYLVF AFEKQQ

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB168817 mRNA. Translation: BAE00921.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q4R7K1.
Enzyme and pathway databases
BRENDA	2.1.1.56. 3438.
Family and domain databases
InterPro	IPR016899. mRNA_G-N7_MeTrfase. IPR004971. Pox_MCEL. [Graphical view]
Pfam	PF03291. Pox_MCEL. 1 hit. [Graphical view]
PIRSF	PIRSF028762. ABD1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCES_MACFA

Accession

Primary (citable) accession number: Q4R7K1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	July 19, 2005
Last modified:	January 20, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)