Reviewed, UniProtKB/Swiss-Prot Q4R755 (GPDM_MACFA)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate dehydrogenase, mitochondrial
      Short name=GPDH-M
      Short name=GPD-M
    EC=1.1.5.3
Alternative name(s):
    mtGPD

Gene names

Name:	GPD2
ORF Names:	QtsA-16270

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	727 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.
Cofactor	FAD By similarity.
Enzyme regulation	Calcium-binding enhance the activity of the enzyme By similarity.
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (aerobic route): step 1/1.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family. Contains 2 EF-hand domains.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Repeat Transit peptide
Ligand	Calcium FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol-3-phosphate dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 42	42	Mitochondrion By similarity
	Chain	43 – 727	685	Glycerol-3-phosphate dehydrogenase, mitochondrial		PRO_0000270069
Regions
	Domain	623 – 658	36	EF-hand 1
	Domain	659 – 694	36	EF-hand 2
	Nucleotide binding	71 – 99	29	FAD Potential
	Calcium binding	672 – 683	12	Potential
Amino acid modifications
	Modified residue	600	1	Phosphotyrosine By similarity
	Modified residue	601	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4R755-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: CFB9E66271721B66	FASTA	727	80,801
	10 20 30 40 50 60 MAFQKAVKGT ILVGGGALAT VLGLSQFAHY RRKQMNLAYV KAADYISEPV NREPPSREAQ 70 80 90 100 110 120 LLTLQNTSEF DILVIGGGAT GSGCALDAVT RGLKTALVER DDFSSGTSSR STKLIHGGVR 130 140 150 160 170 180 YLQKAIMKLD IEQYRMVKEA LHERANLLEI APHLSAPLPI VLPVYKWWQL PYYWVGIKLY 190 200 210 220 230 240 DLVAGSNCLK SSYVLSKSRA LEHFPMLQKD KLVGAIVYYD GQHNDARMNL AIALTAARYG 250 260 270 280 290 300 AATANYMEVV SLLKKTDPQT GKVRVSGARC KDVLTGQEFD VRAKCVINAT GPFTDSVRKM 310 320 330 340 350 360 DDKDAAAICQ PSAGVHIVMP GYYSPESMGL LDPATSDGRV IFFLPWQKMT IAGTTDTPTD 370 380 390 400 410 420 VTPHPIPSEE DINFILNEVR NYLSCDVEVR RGDVLAAWSG IRPLVTDPKS ADTQSISRNH 430 440 450 460 470 480 VVDISESGLI TIAGGKWTTY RSMAEDTINA AIKTHNLKAG PSRTVGLFLQ GGKDWSPTLY 490 500 510 520 530 540 IRLVQDYGLE SEVAQHLAAT YGDKAFEVAK MASVTGKRWP IVGVRLVSEF PYIEAEVKYG 550 560 570 580 590 600 IKEYACTAVD MISRRTRLAF LNVQAAEEAL PRIVELMGRE LNWDDHKKQE QLETAKKFLY 610 620 630 640 650 660 YEMGYKSRSE QLTDRSEISL LPSDIDRYKK RFHKFDADKK GFITIVDVQR VLESINVQMD 670 680 690 700 710 720 ENTLHEILNE VDLNKNGQVE LNEFLQLMSA IQKGRVSGSR LAILMKTAEE NLDRRVPIPV DRSCGGL

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB168970 mRNA. Translation: BAE01068.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q4R755.
Enzyme and pathway databases
BRENDA	1.1.99.5. 3438.
Family and domain databases
InterPro	IPR011992. EF-Hand_type. IPR018248. EF_hand. IPR018247. EF_HAND_1. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. IPR000447. FAD-dep_Gly3P_DH. IPR006076. FAD-dep_OxRdtase. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
Pfam	PF01266. DAO. 1 hit. PF00036. efhand. 1 hit. [Graphical view]
PRINTS	PR01001. FADG3PDH.
ProDom	PD000012. EF-hand. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 2 hits. PS00977. FAD_G3PDH_1. 1 hit. PS00978. FAD_G3PDH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPDM_MACFA

Accession

Primary (citable) accession number: Q4R755

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	July 19, 2005
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)