Reviewed, UniProtKB/Swiss-Prot Q4R741 (RIR2B_MACFA)

Names and origin

Protein names

Recommended name:
    Ribonucleoside-diphosphate reductase subunit M2 B
    EC=1.17.4.1

Gene names

Name:	RRM2B
ORF Names:	QtsA-16381

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Cofactor	Binds 2 iron ions per subunit By similarity.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Heterotetramer with large (RRM1) subunit. Interacts with p53/TP53. Interacts with RRM1 in response to DNA damage. DNA damage By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: Translocates from cytoplasm to nucleus in response to DNA damage By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase small chain family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm Nucleus
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW deoxyribonucleoside diphosphate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside-diphosphate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Ribonucleoside-diphosphate reductase subunit M2 B		PRO_0000228151
Sites
	Active site	138	1	By similarity
	Metal binding	100	1	Iron 1 By similarity
	Metal binding	131	1	Iron 1 By similarity
	Metal binding	131	1	Iron 2 By similarity
	Metal binding	134	1	Iron 1 By similarity
	Metal binding	194	1	Iron 2 By similarity
	Metal binding	228	1	Iron 2 By similarity
	Metal binding	231	1	Iron 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4R741-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 9887F862C3FD498A	FASTA	351	40,763
	10 20 30 40 50 60 MGDPERPEAA GLDLDERSSS DTNDNEIKSN EEPLLRKSSR RFVIFPIQYP DIWKMYKQAQ 70 80 90 100 110 120 ASFWTAEEVD LSKDLPHWNK LKADEKYFIS HILAFFAASD GIVNENLVER FSQEVQVPEA 130 140 150 160 170 180 RCFYGFQILI ENVHSEMYSL LIDTYIRDPK KREFLFNAIE TMPYVKKKAD WALRWIADRK 190 200 210 220 230 240 STFGERVVAF AAVEGIFFSG SFAAIFWLKK RGLMPGLTFS NELISRDEGL HCDFACLMFQ 250 260 270 280 290 300 YLVNKPSEER VREIIVDAVQ IEQEFLTEAL PVGLIGMNCI LMKQYIEFVA DRLLVELGFS 310 320 330 340 350 KIFQAENPFD FMENISLEGK TNFFEKRVSE YQRFAVMAET TDNVFTLDAD F

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB168988 mRNA. Translation: BAE01083.1.
3D structure databases
SMR	Q4R741. Positions 31-313.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q4R741.
Enzyme and pathway databases
BRENDA	1.17.4.1. 3438.
Family and domain databases
InterPro	IPR012348. Ribncl_red_rel. IPR000358. Ribonucl_redctse. [Graphical view]
Gene3D	G3DSA:1.10.620.20. Ribncl_red_rel. 1 hit.
PANTHER	PTHR23409. Ribonucl_redctse. 1 hit.
Pfam	PF00268. Ribonuc_red_sm. 1 hit. [Graphical view]
PROSITE	PS00368. RIBORED_SMALL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR2B_MACFA

Accession

Primary (citable) accession number: Q4R741

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	July 19, 2005
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)