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Q4R6X7 (UBP16_MACFA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 16

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 16
Ubiquitin thioesterase 16
Ubiquitin-specific-processing protease 16
Gene names
Name:USP16
ORF Names:QtsA-16913, QtsA-18064
OrganismMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifier9541 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Protein attributes

Sequence length826 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B By similarity. HAMAP-Rule MF_03062

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). HAMAP-Rule MF_03062

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_03062

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03062.

Domain

The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin By similarity. HAMAP-Rule MF_03062

Post-translational modification

Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. Phosphorylation by AURKB enhances the deubiquitinase activity By similarity. HAMAP-Rule MF_03062

Sequence similarities

Belongs to the peptidase C19 family. USP16 subfamily.

Contains 1 UBP-type zinc finger.

Contains 1 USP domain.

Sequence caution

The sequence BAE01308.1 differs from that shown. Reason: Frameshift at position 287.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
Transcription
Transcription regulation
Ubl conjugation pathway
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainZinc-finger
   LigandMetal-binding
Zinc
   Molecular functionActivator
Chromatin regulator
Hydrolase
Protease
Thiol protease
   PTMPhosphoprotein
Gene Ontology (GO)
   Biological_processcellular response to DNA damage stimulus

Inferred from sequence or structural similarity. Source: UniProtKB

histone H2A K63-linked deubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

histone deubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

mitosis

Inferred from sequence or structural similarity. Source: UniProtKB

monoubiquitinated histone H2A deubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of translational elongation

Inferred from sequence or structural similarity. Source: UniProtKB

protein homotetramerization

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity. Source: UniProtKB

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentnucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functioncysteine-type endopeptidase activity

Inferred from sequence or structural similarity. Source: UniProtKB

histone binding

Inferred from sequence or structural similarity. Source: UniProtKB

transcription coactivator activity

Inferred from sequence or structural similarity. Source: UniProtKB

ubiquitin binding

Inferred from sequence or structural similarity. Source: UniProtKB

ubiquitin thiolesterase activity

Inferred from sequence or structural similarity. Source: UniProtKB

ubiquitin-specific protease activity

Inferred from sequence or structural similarity. Source: UniProtKB

zinc ion binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q4R6X7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q4R6X7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     150-150: Missing.
     735-826: NVAEENTRVL...AYLLFYERIL → ILKWNQKGSGFTSATHMCKLCLQLKY
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 826826Ubiquitin carboxyl-terminal hydrolase 16 HAMAP-Rule MF_03062
PRO_0000367502

Regions

Domain196 – 825630USP
Zinc finger60 – 12566UBP-type HAMAP-Rule MF_03062

Sites

Active site2051Nucleophile By similarity
Active site7611Proton acceptor By similarity
Metal binding241Zinc 1 By similarity
Metal binding261Zinc 1 By similarity
Metal binding481Zinc 2 By similarity
Metal binding511Zinc 2 By similarity
Metal binding741Zinc 3 By similarity
Metal binding771Zinc 3 By similarity
Metal binding821Zinc 2 By similarity
Metal binding901Zinc 2 By similarity
Metal binding941Zinc 3 By similarity
Metal binding1031Zinc 3 By similarity
Metal binding1161Zinc 1 By similarity
Metal binding1191Zinc 1 By similarity

Amino acid modifications

Modified residue4151Phosphoserine By similarity
Modified residue5521Phosphoserine By similarity
Modified residue5571Phosphothreonine By similarity

Natural variations

Alternative sequence1501Missing in isoform 2.
VSP_036723
Alternative sequence735 – 82692NVAEE…YERIL → ILKWNQKGSGFTSATHMCKL CLQLKY in isoform 2.
VSP_036724

Experimental info

Sequence conflict551N → D in BAE01308. Ref.1
Sequence conflict1281S → P in BAE01308. Ref.1
Sequence conflict1361D → E in BAE01308. Ref.1
Sequence conflict1431S → G in BAE01147. Ref.1
Sequence conflict2081N → D in BAE01308. Ref.1
Sequence conflict5311P → S in BAE01147. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 24, 2009. Version 2.
Checksum: EEBDB3D17A4CD634

FASTA82694,059
        10         20         30         40         50         60 
MGKKRTKGKT VPIDDSSETL EPMCRHIRKG LEQGNLKKAL VNVEWNICQD CKTDNKVKDK 

        70         80         90        100        110        120 
AEEETEEKPS VWLCLKCGHQ GCGRNSQEQH ALKHYLTPRS EPHCLVLSLD NWSVWCYICD 

       130        140        150        160        170        180 
NEVQYCSSNQ LGQVVDYVRK QASITTPKPA EKDNGNIELE NKKLEKESKN EQEREKKENM 

       190        200        210        220        230        240 
AKENPPMNSP SQITVKGLSN LGNTCFFNAV MQNLSQTPVL RELLKEVKMS GTIVKIEPPD 

       250        260        270        280        290        300 
LALTEPLEIN LEPPGPLTLA MSQFLNEMQE TKKGIVTPKE LFSQVCKKAV RFKGYQQQDS 

       310        320        330        340        350        360 
QELLRYLLDG MRAEEHQRVS KGILKAFGNS TEKLDEELKN KVKDYEKKKS IPSFVDRIFG 

       370        380        390        400        410        420 
GELTSTIMCD QCRTVSLVHE SFLDLSLPVL DDQSGKKSVN DKNLKKTMED EDQDSEEEKD 

       430        440        450        460        470        480 
NDSYIKERSD IPSGTSKHLQ KKAKKQAKKQ AKNQRRQQKI QGKVLHLNDI CTIDHPEDNE 

       490        500        510        520        530        540 
YEAEMSLQGE VNIKSNHISQ EGVMHKEYCV NQKDLNGQEK MIESVTDNQK PTEEVDMKNI 

       550        560        570        580        590        600 
NMDNDLEVLT SSPTECTRNL NGAYLTEASN GEVDISNGFK NLNLNAALHP DEINIEILND 

       610        620        630        640        650        660 
SHTPGTKVYE VVNEDPETAF CTLANREVFN TDECSIQHCL YQFTRNEKLR DANKLLCEVC 

       670        680        690        700        710        720 
TRRQCNGPKA NMKGERKHVY TNAKKQMLIS LAPPVLTLHL KRFQQAGFNL RKVNKHIKFP 

       730        740        750        760        770        780 
EILDLAPFCT LKCKNVAEEN TRVLYSLYGV VEHSGTMRSG HYTAYAKART ANSHLSNLVL 

       790        800        810        820 
HGDIPQDFEM ESKGQWFHIS DTHVQAVPTT KVLNSQAYLL FYERIL 

« Hide

Isoform 2 [UniParc].

Checksum: A982B3FAD51D9112
Show »

FASTA75986,532

References

[1]"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
International consortium for macaque cDNA sequencing and analysis
Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Testis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB169053 mRNA. Translation: BAE01147.1.
AB169216 mRNA. Translation: BAE01308.1. Frameshift.
RefSeqXP_005548930.1. XM_005548873.1.
UniGeneMfa.956.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ4R6X7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID101925147.
KEGGmcf:101925147.

Organism-specific databases

CTD10600.

Phylogenomic databases

HOVERGENHBG062704.
KOK11844.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
HAMAPMF_03062. UBP16.
InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBP16_MACFA
AccessionPrimary (citable) accession number: Q4R6X7
Secondary accession number(s): Q4R6G7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 24, 2009
Last modified: April 16, 2014
This is version 48 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries