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Q4R6X7

- UBP16_MACFA

UniProt

Q4R6X7 - UBP16_MACFA

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Protein

Ubiquitin carboxyl-terminal hydrolase 16

Gene

USP16

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.UniRule annotation

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi24 – 241Zinc 1UniRule annotation
Metal bindingi26 – 261Zinc 1UniRule annotation
Metal bindingi48 – 481Zinc 2UniRule annotation
Metal bindingi51 – 511Zinc 2UniRule annotation
Metal bindingi74 – 741Zinc 3UniRule annotation
Metal bindingi77 – 771Zinc 3UniRule annotation
Metal bindingi82 – 821Zinc 2UniRule annotation
Metal bindingi90 – 901Zinc 2UniRule annotation
Metal bindingi94 – 941Zinc 3UniRule annotation
Metal bindingi103 – 1031Zinc 3UniRule annotation
Metal bindingi116 – 1161Zinc 1UniRule annotation
Metal bindingi119 – 1191Zinc 1UniRule annotation
Active sitei205 – 2051NucleophileUniRule annotation
Active sitei761 – 7611Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri60 – 12566UBP-typeUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: UniProtKB
  2. histone binding Source: UniProtKB
  3. transcription coactivator activity Source: UniProtKB
  4. ubiquitin binding Source: UniProtKB
  5. ubiquitin-specific protease activity Source: UniProtKB
  6. ubiquitin thiolesterase activity Source: UniProtKB
  7. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. cellular response to DNA damage stimulus Source: UniProtKB
  2. histone deubiquitination Source: UniProtKB
  3. histone H2A K63-linked deubiquitination Source: UniProtKB
  4. mitotic nuclear division Source: UniProtKB
  5. monoubiquitinated histone H2A deubiquitination Source: UniProtKB
  6. positive regulation of transcription, DNA-templated Source: UniProtKB
  7. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  8. positive regulation of translational elongation Source: UniProtKB
  9. protein homotetramerization Source: UniProtKB
  10. regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  11. transcription, DNA-templated Source: UniProtKB-KW
  12. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 16UniRule annotation (EC:3.4.19.12UniRule annotation)
Alternative name(s):
Deubiquitinating enzyme 16UniRule annotation
Ubiquitin thioesterase 16UniRule annotation
Ubiquitin-specific-processing protease 16UniRule annotation
Gene namesi
Name:USP16UniRule annotation
ORF Names:QtsA-16913, QtsA-18064
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

Nucleus UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 826826Ubiquitin carboxyl-terminal hydrolase 16PRO_0000367502Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei415 – 4151PhosphoserineBy similarity
Modified residuei552 – 5521PhosphoserineBy similarity
Modified residuei557 – 5571PhosphothreonineBy similarity

Post-translational modificationi

Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. Phosphorylation by AURKB enhances the deubiquitinase activity.UniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ4R6X7.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini196 – 825630USPAdd
BLAST

Domaini

The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin.UniRule annotation

Sequence similaritiesi

Belongs to the peptidase C19 family. USP16 subfamily.UniRule annotation
Contains 1 UBP-type zinc finger.UniRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri60 – 12566UBP-typeUniRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOVERGENiHBG062704.
KOiK11844.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
HAMAPiMF_03062. UBP16.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q4R6X7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKKRTKGKT VPIDDSSETL EPMCRHIRKG LEQGNLKKAL VNVEWNICQD
60 70 80 90 100
CKTDNKVKDK AEEETEEKPS VWLCLKCGHQ GCGRNSQEQH ALKHYLTPRS
110 120 130 140 150
EPHCLVLSLD NWSVWCYICD NEVQYCSSNQ LGQVVDYVRK QASITTPKPA
160 170 180 190 200
EKDNGNIELE NKKLEKESKN EQEREKKENM AKENPPMNSP SQITVKGLSN
210 220 230 240 250
LGNTCFFNAV MQNLSQTPVL RELLKEVKMS GTIVKIEPPD LALTEPLEIN
260 270 280 290 300
LEPPGPLTLA MSQFLNEMQE TKKGIVTPKE LFSQVCKKAV RFKGYQQQDS
310 320 330 340 350
QELLRYLLDG MRAEEHQRVS KGILKAFGNS TEKLDEELKN KVKDYEKKKS
360 370 380 390 400
IPSFVDRIFG GELTSTIMCD QCRTVSLVHE SFLDLSLPVL DDQSGKKSVN
410 420 430 440 450
DKNLKKTMED EDQDSEEEKD NDSYIKERSD IPSGTSKHLQ KKAKKQAKKQ
460 470 480 490 500
AKNQRRQQKI QGKVLHLNDI CTIDHPEDNE YEAEMSLQGE VNIKSNHISQ
510 520 530 540 550
EGVMHKEYCV NQKDLNGQEK MIESVTDNQK PTEEVDMKNI NMDNDLEVLT
560 570 580 590 600
SSPTECTRNL NGAYLTEASN GEVDISNGFK NLNLNAALHP DEINIEILND
610 620 630 640 650
SHTPGTKVYE VVNEDPETAF CTLANREVFN TDECSIQHCL YQFTRNEKLR
660 670 680 690 700
DANKLLCEVC TRRQCNGPKA NMKGERKHVY TNAKKQMLIS LAPPVLTLHL
710 720 730 740 750
KRFQQAGFNL RKVNKHIKFP EILDLAPFCT LKCKNVAEEN TRVLYSLYGV
760 770 780 790 800
VEHSGTMRSG HYTAYAKART ANSHLSNLVL HGDIPQDFEM ESKGQWFHIS
810 820
DTHVQAVPTT KVLNSQAYLL FYERIL
Length:826
Mass (Da):94,059
Last modified:March 24, 2009 - v2
Checksum:iEEBDB3D17A4CD634
GO
Isoform 2 (identifier: Q4R6X7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     150-150: Missing.
     735-826: NVAEENTRVL...AYLLFYERIL → ILKWNQKGSGFTSATHMCKLCLQLKY

Note: No experimental confirmation available.

Show »
Length:759
Mass (Da):86,532
Checksum:iA982B3FAD51D9112
GO

Sequence cautioni

The sequence BAE01308.1 differs from that shown. Reason: Frameshift at position 287.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti55 – 551N → D in BAE01308. 1 PublicationCurated
Sequence conflicti128 – 1281S → P in BAE01308. 1 PublicationCurated
Sequence conflicti136 – 1361D → E in BAE01308. 1 PublicationCurated
Sequence conflicti143 – 1431S → G in BAE01147. 1 PublicationCurated
Sequence conflicti208 – 2081N → D in BAE01308. 1 PublicationCurated
Sequence conflicti531 – 5311P → S in BAE01147. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei150 – 1501Missing in isoform 2. 1 PublicationVSP_036723
Alternative sequencei735 – 82692NVAEE…YERIL → ILKWNQKGSGFTSATHMCKL CLQLKY in isoform 2. 1 PublicationVSP_036724Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB169053 mRNA. Translation: BAE01147.1.
AB169216 mRNA. Translation: BAE01308.1. Frameshift.
RefSeqiXP_005548930.1. XM_005548873.1.
UniGeneiMfa.956.

Genome annotation databases

GeneIDi101925147.
KEGGimcf:101925147.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB169053 mRNA. Translation: BAE01147.1 .
AB169216 mRNA. Translation: BAE01308.1 . Frameshift.
RefSeqi XP_005548930.1. XM_005548873.1.
UniGenei Mfa.956.

3D structure databases

ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q4R6X7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 101925147.
KEGGi mcf:101925147.

Organism-specific databases

CTDi 10600.

Phylogenomic databases

HOVERGENi HBG062704.
KOi K11844.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
HAMAPi MF_03062. UBP16.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
    International consortium for macaque cDNA sequencing and analysis
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Testis.

Entry informationi

Entry nameiUBP16_MACFA
AccessioniPrimary (citable) accession number: Q4R6X7
Secondary accession number(s): Q4R6G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 24, 2009
Last modified: October 1, 2014
This is version 50 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3