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Q4R6X7

- UBP16_MACFA

UniProt

Q4R6X7 - UBP16_MACFA

Protein

Ubiquitin carboxyl-terminal hydrolase 16

Gene

USP16

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 2 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.UniRule annotation

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi24 – 241Zinc 1UniRule annotation
    Metal bindingi26 – 261Zinc 1UniRule annotation
    Metal bindingi48 – 481Zinc 2UniRule annotation
    Metal bindingi51 – 511Zinc 2UniRule annotation
    Metal bindingi74 – 741Zinc 3UniRule annotation
    Metal bindingi77 – 771Zinc 3UniRule annotation
    Metal bindingi82 – 821Zinc 2UniRule annotation
    Metal bindingi90 – 901Zinc 2UniRule annotation
    Metal bindingi94 – 941Zinc 3UniRule annotation
    Metal bindingi103 – 1031Zinc 3UniRule annotation
    Metal bindingi116 – 1161Zinc 1UniRule annotation
    Metal bindingi119 – 1191Zinc 1UniRule annotation
    Active sitei205 – 2051NucleophileUniRule annotation
    Active sitei761 – 7611Proton acceptorUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri60 – 12566UBP-typeUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: UniProtKB
    2. histone binding Source: UniProtKB
    3. transcription coactivator activity Source: UniProtKB
    4. ubiquitin binding Source: UniProtKB
    5. ubiquitin-specific protease activity Source: UniProtKB
    6. ubiquitin thiolesterase activity Source: UniProtKB
    7. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: UniProtKB
    2. histone deubiquitination Source: UniProtKB
    3. histone H2A K63-linked deubiquitination Source: UniProtKB
    4. mitotic nuclear division Source: UniProtKB
    5. monoubiquitinated histone H2A deubiquitination Source: UniProtKB
    6. positive regulation of transcription, DNA-templated Source: UniProtKB
    7. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    8. positive regulation of translational elongation Source: UniProtKB
    9. protein homotetramerization Source: UniProtKB
    10. regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    11. transcription, DNA-templated Source: UniProtKB-KW
    12. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Activator, Chromatin regulator, Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 16UniRule annotation (EC:3.4.19.12UniRule annotation)
    Alternative name(s):
    Deubiquitinating enzyme 16UniRule annotation
    Ubiquitin thioesterase 16UniRule annotation
    Ubiquitin-specific-processing protease 16UniRule annotation
    Gene namesi
    Name:USP16UniRule annotation
    ORF Names:QtsA-16913, QtsA-18064
    OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
    Taxonomic identifieri9541 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

    Subcellular locationi

    Nucleus UniRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 826826Ubiquitin carboxyl-terminal hydrolase 16PRO_0000367502Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei415 – 4151PhosphoserineBy similarity
    Modified residuei552 – 5521PhosphoserineBy similarity
    Modified residuei557 – 5571PhosphothreonineBy similarity

    Post-translational modificationi

    Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. Phosphorylation by AURKB enhances the deubiquitinase activity.UniRule annotation

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ4R6X7.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini196 – 825630USPAdd
    BLAST

    Domaini

    The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin.UniRule annotation

    Sequence similaritiesi

    Belongs to the peptidase C19 family. USP16 subfamily.UniRule annotation
    Contains 1 UBP-type zinc finger.UniRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri60 – 12566UBP-typeUniRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    HOVERGENiHBG062704.
    KOiK11844.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    HAMAPiMF_03062. UBP16.
    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q4R6X7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGKKRTKGKT VPIDDSSETL EPMCRHIRKG LEQGNLKKAL VNVEWNICQD    50
    CKTDNKVKDK AEEETEEKPS VWLCLKCGHQ GCGRNSQEQH ALKHYLTPRS 100
    EPHCLVLSLD NWSVWCYICD NEVQYCSSNQ LGQVVDYVRK QASITTPKPA 150
    EKDNGNIELE NKKLEKESKN EQEREKKENM AKENPPMNSP SQITVKGLSN 200
    LGNTCFFNAV MQNLSQTPVL RELLKEVKMS GTIVKIEPPD LALTEPLEIN 250
    LEPPGPLTLA MSQFLNEMQE TKKGIVTPKE LFSQVCKKAV RFKGYQQQDS 300
    QELLRYLLDG MRAEEHQRVS KGILKAFGNS TEKLDEELKN KVKDYEKKKS 350
    IPSFVDRIFG GELTSTIMCD QCRTVSLVHE SFLDLSLPVL DDQSGKKSVN 400
    DKNLKKTMED EDQDSEEEKD NDSYIKERSD IPSGTSKHLQ KKAKKQAKKQ 450
    AKNQRRQQKI QGKVLHLNDI CTIDHPEDNE YEAEMSLQGE VNIKSNHISQ 500
    EGVMHKEYCV NQKDLNGQEK MIESVTDNQK PTEEVDMKNI NMDNDLEVLT 550
    SSPTECTRNL NGAYLTEASN GEVDISNGFK NLNLNAALHP DEINIEILND 600
    SHTPGTKVYE VVNEDPETAF CTLANREVFN TDECSIQHCL YQFTRNEKLR 650
    DANKLLCEVC TRRQCNGPKA NMKGERKHVY TNAKKQMLIS LAPPVLTLHL 700
    KRFQQAGFNL RKVNKHIKFP EILDLAPFCT LKCKNVAEEN TRVLYSLYGV 750
    VEHSGTMRSG HYTAYAKART ANSHLSNLVL HGDIPQDFEM ESKGQWFHIS 800
    DTHVQAVPTT KVLNSQAYLL FYERIL 826
    Length:826
    Mass (Da):94,059
    Last modified:March 24, 2009 - v2
    Checksum:iEEBDB3D17A4CD634
    GO
    Isoform 2 (identifier: Q4R6X7-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         150-150: Missing.
         735-826: NVAEENTRVL...AYLLFYERIL → ILKWNQKGSGFTSATHMCKLCLQLKY

    Note: No experimental confirmation available.

    Show »
    Length:759
    Mass (Da):86,532
    Checksum:iA982B3FAD51D9112
    GO

    Sequence cautioni

    The sequence BAE01308.1 differs from that shown. Reason: Frameshift at position 287.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti55 – 551N → D in BAE01308. 1 PublicationCurated
    Sequence conflicti128 – 1281S → P in BAE01308. 1 PublicationCurated
    Sequence conflicti136 – 1361D → E in BAE01308. 1 PublicationCurated
    Sequence conflicti143 – 1431S → G in BAE01147. 1 PublicationCurated
    Sequence conflicti208 – 2081N → D in BAE01308. 1 PublicationCurated
    Sequence conflicti531 – 5311P → S in BAE01147. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei150 – 1501Missing in isoform 2. 1 PublicationVSP_036723
    Alternative sequencei735 – 82692NVAEE…YERIL → ILKWNQKGSGFTSATHMCKL CLQLKY in isoform 2. 1 PublicationVSP_036724Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB169053 mRNA. Translation: BAE01147.1.
    AB169216 mRNA. Translation: BAE01308.1. Frameshift.
    RefSeqiXP_005548930.1. XM_005548873.1.
    UniGeneiMfa.956.

    Genome annotation databases

    GeneIDi101925147.
    KEGGimcf:101925147.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB169053 mRNA. Translation: BAE01147.1 .
    AB169216 mRNA. Translation: BAE01308.1 . Frameshift.
    RefSeqi XP_005548930.1. XM_005548873.1.
    UniGenei Mfa.956.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q4R6X7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 101925147.
    KEGGi mcf:101925147.

    Organism-specific databases

    CTDi 10600.

    Phylogenomic databases

    HOVERGENi HBG062704.
    KOi K11844.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    HAMAPi MF_03062. UBP16.
    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
      International consortium for macaque cDNA sequencing and analysis
      Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Testis.

    Entry informationi

    Entry nameiUBP16_MACFA
    AccessioniPrimary (citable) accession number: Q4R6X7
    Secondary accession number(s): Q4R6G7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 50 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3