Q4R6X7 (UBP16_MACFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 16
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 16
Ubiquitin thioesterase 16
Ubiquitin-specific-processing protease 16

Gene names

Name:	USP16
ORF Names:	QtsA-16913, QtsA-18064

Organism

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Cercopithecoidea › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	826 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Specifically deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-10' of histone H3, and is required for chromosome segregation when cells enter into mitosis. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit structure	Homotetramer By similarity.
Subcellular location	Nucleus By similarity.
Domain	The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin By similarity.
Post-translational modification	Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. The phosphorylated form of the protein is also enzymatically active By similarity.
Sequence similarities	Belongs to the peptidase C19 family. USP16 subfamily. Contains 1 UBP-type zinc finger.
Sequence caution	The sequence BAE01308.1 differs from that shown. Reason: Frameshift at position 287.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Activator Chromatin regulator Hydrolase Protease Thiol protease
PTM	Phosphoprotein
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW histone H2A K63-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB mitosis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of translational elongation Inferred from sequence or structural similarity. Source: UniProtKB protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB response to DNA damage stimulus Inferred from sequence or structural similarity. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	cysteine-type endopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB histone binding Inferred from sequence or structural similarity. Source: UniProtKB transcription coactivator activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin binding Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin thiolesterase activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q4R6X7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q4R6X7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     150-150: Missing.
     735-826: NVAEENTRVL...AYLLFYERIL → ILKWNQKGSGFTSATHMCKLCLQLKY

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 826	826	Ubiquitin carboxyl-terminal hydrolase 16		PRO_0000367502
Regions
	Zinc finger	60 – 125	66	UBP-type
Sites
	Active site	205	1	Nucleophile By similarity
	Active site	761	1	Proton acceptor By similarity
	Metal binding	24	1	Zinc 1 By similarity
	Metal binding	26	1	Zinc 1 By similarity
	Metal binding	48	1	Zinc 2 By similarity
	Metal binding	51	1	Zinc 2 By similarity
	Metal binding	74	1	Zinc 3 By similarity
	Metal binding	77	1	Zinc 3 By similarity
	Metal binding	82	1	Zinc 2 By similarity
	Metal binding	88	1	Zinc 2 By similarity
	Metal binding	90	1	Zinc 2 By similarity
	Metal binding	94	1	Zinc 3 By similarity
	Metal binding	103	1	Zinc 3 By similarity
	Metal binding	116	1	Zinc 1 By similarity
	Metal binding	119	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	415	1	Phosphoserine By similarity
	Modified residue	552	1	Phosphoserine By similarity
	Modified residue	557	1	Phosphothreonine By similarity
Natural variations
	Alternative sequence	150	1	Missing in isoform 2.		VSP_036723
	Alternative sequence	735 – 826	92	NVAEE…YERIL → ILKWNQKGSGFTSATHMCKL CLQLKY in isoform 2.		VSP_036724
Experimental info
	Sequence conflict	55	1	N → D in BAE01308. Ref.1
	Sequence conflict	128	1	S → P in BAE01308. Ref.1
	Sequence conflict	136	1	D → E in BAE01308. Ref.1
	Sequence conflict	143	1	S → G in BAE01147. Ref.1
	Sequence conflict	208	1	N → D in BAE01308. Ref.1
	Sequence conflict	531	1	P → S in BAE01147. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 24, 2009. Version 2. Checksum: EEBDB3D17A4CD634	FASTA	826	94,059
	10 20 30 40 50 60 MGKKRTKGKT VPIDDSSETL EPMCRHIRKG LEQGNLKKAL VNVEWNICQD CKTDNKVKDK 70 80 90 100 110 120 AEEETEEKPS VWLCLKCGHQ GCGRNSQEQH ALKHYLTPRS EPHCLVLSLD NWSVWCYICD 130 140 150 160 170 180 NEVQYCSSNQ LGQVVDYVRK QASITTPKPA EKDNGNIELE NKKLEKESKN EQEREKKENM 190 200 210 220 230 240 AKENPPMNSP SQITVKGLSN LGNTCFFNAV MQNLSQTPVL RELLKEVKMS GTIVKIEPPD 250 260 270 280 290 300 LALTEPLEIN LEPPGPLTLA MSQFLNEMQE TKKGIVTPKE LFSQVCKKAV RFKGYQQQDS 310 320 330 340 350 360 QELLRYLLDG MRAEEHQRVS KGILKAFGNS TEKLDEELKN KVKDYEKKKS IPSFVDRIFG 370 380 390 400 410 420 GELTSTIMCD QCRTVSLVHE SFLDLSLPVL DDQSGKKSVN DKNLKKTMED EDQDSEEEKD 430 440 450 460 470 480 NDSYIKERSD IPSGTSKHLQ KKAKKQAKKQ AKNQRRQQKI QGKVLHLNDI CTIDHPEDNE 490 500 510 520 530 540 YEAEMSLQGE VNIKSNHISQ EGVMHKEYCV NQKDLNGQEK MIESVTDNQK PTEEVDMKNI 550 560 570 580 590 600 NMDNDLEVLT SSPTECTRNL NGAYLTEASN GEVDISNGFK NLNLNAALHP DEINIEILND 610 620 630 640 650 660 SHTPGTKVYE VVNEDPETAF CTLANREVFN TDECSIQHCL YQFTRNEKLR DANKLLCEVC 670 680 690 700 710 720 TRRQCNGPKA NMKGERKHVY TNAKKQMLIS LAPPVLTLHL KRFQQAGFNL RKVNKHIKFP 730 740 750 760 770 780 EILDLAPFCT LKCKNVAEEN TRVLYSLYGV VEHSGTMRSG HYTAYAKART ANSHLSNLVL 790 800 810 820 HGDIPQDFEM ESKGQWFHIS DTHVQAVPTT KVLNSQAYLL FYERIL
	Isoform 2 [UniParc]. Checksum: A982B3FAD51D9112	FASTA	759	86,532
	10 20 30 40 50 60 MGKKRTKGKT VPIDDSSETL EPMCRHIRKG LEQGNLKKAL VNVEWNICQD CKTDNKVKDK 70 80 90 100 110 120 AEEETEEKPS VWLCLKCGHQ GCGRNSQEQH ALKHYLTPRS EPHCLVLSLD NWSVWCYICD 130 140 150 160 170 180 NEVQYCSSNQ LGQVVDYVRK QASITTPKPE KDNGNIELEN KKLEKESKNE QEREKKENMA 190 200 210 220 230 240 KENPPMNSPS QITVKGLSNL GNTCFFNAVM QNLSQTPVLR ELLKEVKMSG TIVKIEPPDL 250 260 270 280 290 300 ALTEPLEINL EPPGPLTLAM SQFLNEMQET KKGIVTPKEL FSQVCKKAVR FKGYQQQDSQ 310 320 330 340 350 360 ELLRYLLDGM RAEEHQRVSK GILKAFGNST EKLDEELKNK VKDYEKKKSI PSFVDRIFGG 370 380 390 400 410 420 ELTSTIMCDQ CRTVSLVHES FLDLSLPVLD DQSGKKSVND KNLKKTMEDE DQDSEEEKDN 430 440 450 460 470 480 DSYIKERSDI PSGTSKHLQK KAKKQAKKQA KNQRRQQKIQ GKVLHLNDIC TIDHPEDNEY 490 500 510 520 530 540 EAEMSLQGEV NIKSNHISQE GVMHKEYCVN QKDLNGQEKM IESVTDNQKP TEEVDMKNIN 550 560 570 580 590 600 MDNDLEVLTS SPTECTRNLN GAYLTEASNG EVDISNGFKN LNLNAALHPD EINIEILNDS 610 620 630 640 650 660 HTPGTKVYEV VNEDPETAFC TLANREVFNT DECSIQHCLY QFTRNEKLRD ANKLLCEVCT 670 680 690 700 710 720 RRQCNGPKAN MKGERKHVYT NAKKQMLISL APPVLTLHLK RFQQAGFNLR KVNKHIKFPE 730 740 750 ILDLAPFCTL KCKILKWNQK GSGFTSATHM CKLCLQLKY

References

[1]

"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB169053 mRNA. Translation: BAE01147.1. AB169216 mRNA. Translation: BAE01308.1. Frameshift.
3D structure databases
HSSP	HSSP built from PDB template 2GFO based on UniProtKB P40818.
ModBase	Search...
Proteomic databases
PRIDE	Q4R6X7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG062704.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR013083. Znf_RING/FYVE/PHD. IPR001607. Znf_UBP. [Graphical view]
Pfam	PF00443. UCH. 1 hit. PF02148. zf-UBP. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. PS50271. ZF_UBP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP16_MACFA

Accession

Primary (citable) accession number: Q4R6X7
Secondary accession number(s): Q4R6G7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	April 3, 2013
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program