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Q4R6L3

- PLCD4_MACFA

UniProt

Q4R6L3 - PLCD4_MACFA

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4

Gene

PLCD4

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (19 Jul 2005)
      Previous versions | rss
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    Functioni

    Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca2+ from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca2+ mobilization in the zona pellucida-induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation By similarity.By similarity

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Cofactori

    Binds 3 calcium ions per subunit. Two of the calcium ions are bound to the C2 domain By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei305 – 3051PROSITE-ProRule annotation
    Metal bindingi306 – 3061Calcium 1; catalyticBy similarity
    Metal bindingi335 – 3351Calcium 1; catalyticBy similarity
    Metal bindingi337 – 3371Calcium 1; catalyticBy similarity
    Active sitei350 – 3501PROSITE-ProRule annotation
    Metal bindingi384 – 3841Calcium 1; catalyticBy similarity
    Binding sitei433 – 4331SubstrateBy similarity
    Binding sitei435 – 4351SubstrateBy similarity
    Binding sitei559 – 5591SubstrateBy similarity
    Binding sitei586 – 5861SubstrateBy similarity
    Metal bindingi687 – 6871Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi689 – 6891Calcium 2By similarity
    Metal bindingi713 – 7131Calcium 2By similarity
    Metal bindingi742 – 7421Calcium 3By similarity
    Metal bindingi743 – 7431Calcium 3; via carbonyl oxygenBy similarity
    Metal bindingi744 – 7441Calcium 3By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi147 – 158121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi183 – 194122PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
    3. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. acrosome reaction Source: InterPro
    2. intracellular signal transduction Source: InterPro
    3. lipid catabolic process Source: UniProtKB-KW
    4. phosphatidylinositol metabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase C-delta-4
    Phospholipase C-delta-4
    Short name:
    PLC-delta-4
    Gene namesi
    Name:PLCD4
    ORF Names:QtsA-17742
    OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
    Taxonomic identifieri9541 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

    Subcellular locationi

    Membrane By similarity; Peripheral membrane protein By similarity. Nucleus By similarity. Cytoplasm By similarity. Endoplasmic reticulum By similarity
    Note: Localizes primarily to intracellular membranes mostly to the endoplasmic reticulum.By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB-SubCell
    2. membrane Source: UniProtKB-SubCell
    3. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7997991-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4PRO_0000306825Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with GRIP1.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ4R6L3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini16 – 124109PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini134 – 16936EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini170 – 20536EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini203 – 23735EF-hand 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini290 – 435146PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini530 – 646117PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini651 – 756106C2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni26 – 5328Substrate bindingBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi768 – 7714PDZ-binding

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi440 – 47839Glu-richAdd
    BLAST

    Domaini

    The PDZ-binding motif mediates the interaction with GRIP1.By similarity
    The C2 domain mediates pre-localization to the membrane prior to Ca2+ import and non-selective Ca2+-mediated targeting to various cellular membranes.By similarity
    The PH domain is not a critical determinant of the membrane localization.By similarity

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 3 EF-hand domains.PROSITE-ProRule annotation
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    HOVERGENiHBG053610.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    2.30.29.30. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001192. PI-PLC_fam.
    IPR028387. PLC-delta4.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF31. PTHR10336:SF31. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view]
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00054. EFh. 3 hits.
    SM00233. PH. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 2 hits.
    PROSITEiPS50004. C2. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 3 hits.
    PS50003. PH_DOMAIN. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4R6L3-1 [UniParc]FASTAAdd to Basket

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    MASLLQDRLT TDQDLLLMQE GMPMRKVRSK SWKKLRYFRL QNDGMTVWHA    50
    RQARGSAKPS FSISDVETIR NGHDSELLRS LTEELPLEQG FTVVFHGRRS 100
    NLDLVANSVE EAQIWMRGLH LLVDLVTSMD HQERLDQWLS DWFQRGDKNQ 150
    DGKMSFQEVQ RLLHLMNVEM DQEYAFSLFQ AADTSQSGTL EGEEFVEFYK 200
    ALTKRAEVQE LFESFSADGQ KLTLLEFSDF LREEQKERDC TSELALELID 250
    RYEPSDSGKL RHVLSMDGFL SYLCSKDGDI FNPACLPIYQ DMTQPLNHYF 300
    ICSSHNTYLV GDQLCGQSSV EGYIRALKRG CRCVEVDVWD GPSGEPVVYH 350
    GHTLTSRILF KDVVATVAQY AFQTSDYPVI LSLETHCSWE QQQTMARHLT 400
    EILGEQLLST TLDGVLPTQL PSPEELRRKI LVKGKKLTLE EDLEYEEEEV 450
    EPGLEGEHES ELALESQFET ESEPEPQEQN LQIKDKKKVV TCPLFCPSIC 500
    CQIVAQAPIS KPGSLLLSQQ KSKTILCPAL SSLVIYLKSV SFRSFTHSKK 550
    HYHFYEISSF SETKAKRLIK EAGNEFVQHN TWQLSRVYPS GLRTDSSNYN 600
    PQELWNAGCQ MVAMNMQTAG LEMDICDGHF RQNGGCGYVL KPDFLRDNQS 650
    SFHPERPISP FKAQTLLIQV ISGQQLPKLN KTKEGSIVDP LVKVQIFGVR 700
    LDTARQETNY VENNGFNPYW GQTLCFRVLV PELAMLRFVV MDYDWKSRND 750
    FIGQYTLPWS CMQQGYRHIH LLSKDGISLC PASIFVYICI REGLEGDES 799
    Length:799
    Mass (Da):91,437
    Last modified:July 19, 2005 - v1
    Checksum:iFE30A582F5FB7FAF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB169170 mRNA. Translation: BAE01262.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB169170 mRNA. Translation: BAE01262.1 .

    3D structure databases

    ProteinModelPortali Q4R6L3.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG053610.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    2.30.29.30. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001192. PI-PLC_fam.
    IPR028387. PLC-delta4.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF31. PTHR10336:SF31. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view ]
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00054. EFh. 3 hits.
    SM00233. PH. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 2 hits.
    PROSITEi PS50004. C2. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 3 hits.
    PS50003. PH_DOMAIN. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
      International consortium for macaque cDNA sequencing and analysis
      Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiPLCD4_MACFA
    AccessioniPrimary (citable) accession number: Q4R6L3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: July 19, 2005
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3