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Q4R6G8

- EIF3E_MACFA

UniProt

Q4R6G8 - EIF3E_MACFA

Protein

Eukaryotic translation initiation factor 3 subunit E

Gene

EIF3E

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (19 Jul 2005)
      Previous versions | rss
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    Functioni

    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.UniRule annotation

    GO - Molecular functioni

    1. translation initiation factor activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. formation of translation preinitiation complex Source: UniProtKB-HAMAP
    2. nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB
    3. regulation of translational initiation Source: UniProtKB-HAMAP
    4. translational initiation Source: UniProtKB

    Keywords - Molecular functioni

    Initiation factor

    Keywords - Biological processi

    Protein biosynthesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Eukaryotic translation initiation factor 3 subunit EUniRule annotation
    Short name:
    eIF3eUniRule annotation
    Alternative name(s):
    Eukaryotic translation initiation factor 3 subunit 6UniRule annotation
    eIF-3 p48UniRule annotation
    Gene namesi
    Name:EIF3EUniRule annotation
    Synonyms:EIF3S6UniRule annotation, INT6UniRule annotation
    ORF Names:QtsA-10282, QtsA-18056
    OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
    Taxonomic identifieri9541 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

    Subcellular locationi

    Cytoplasm UniRule annotation. NucleusPML body UniRule annotation

    GO - Cellular componenti

    1. eukaryotic 43S preinitiation complex Source: UniProtKB-HAMAP
    2. eukaryotic 48S preinitiation complex Source: UniProtKB-HAMAP
    3. eukaryotic translation initiation factor 3 complex Source: UniProtKB
    4. PML body Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedUniRule annotation
    Chaini2 – 445444Eukaryotic translation initiation factor 3 subunit EPRO_0000291872Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineUniRule annotation
    Modified residuei399 – 3991PhosphoserineBy similarity
    Modified residuei439 – 4391PhosphothreonineBy similarity
    Modified residuei445 – 4451PhosphotyrosineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiQ4R6G8.

    Interactioni

    Subunit structurei

    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with COPS3, COPS6, COPS7 (COPS7A or COPS7B), EIF4G1, EPAS1, MCM7, NCBP1, PSMC6, TRIM27 and UPF2 By similarity. Interacts with IFIT1 and IFIT2 By similarity.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ4R6G8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini290 – 395106PCIUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni4 – 128125Sufficient for interaction with EPAS1UniRule annotationAdd
    BLAST
    Regioni9 – 195187Sufficient for interaction with TRIM27UniRule annotationAdd
    BLAST
    Regioni351 – 44595Sufficient for interaction with MCM7UniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the eIF-3 subunit E family.UniRule annotation
    Contains 1 PCI domain.UniRule annotation

    Phylogenomic databases

    HOVERGENiHBG001097.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    HAMAPiMF_03004. eIF3e.
    InterProiIPR016650. eIF3e.
    IPR019010. eIF3e_N.
    IPR000717. PCI_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PANTHERiPTHR10317. PTHR10317. 1 hit.
    PfamiPF09440. eIF3_N. 1 hit.
    PF01399. PCI. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016255. eIF3e_su6. 1 hit.
    SMARTiSM00088. PINT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q4R6G8-1 [UniParc]FASTAAdd to Basket

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    MAEYDLTTRI AHFLDRHLVF PLLEFLSVKE IYNEKELLQG KLDLLSDTNM    50
    VDFAMDVYKN LYSDDIPHAL REKRTTVVAQ LKQLQAETEP IVKMFEDPET 100
    TRQMQSTRDG RMLFDYLADK HGFRQEYLDT LYRYAKFQYE CGNYSGAAEY 150
    LYFFRVLVPA TDRNALSSLW GKLASEILMQ NWDAAMEDLT RLKETIDNNS 200
    VSSPLQSLQQ RTWLIHWSLF VFFNHPKGRD NIIDLFLYQP QYLNAIQTMC 250
    PHILRYLTTA VITNKDVRKR RQVLKDLVKV IQQESYTYKD PITEFVECLY 300
    VNFDFDGAQK KLRECESVLV NDFFLVACLE DFIENARLFI FETFCRIHQC 350
    ISINMLADKL NMTPEEAERW IVNLIRNARL DAKIDSKLGH VVMGNNAVSP 400
    YQQVIEKTKS LSFRSQMLAM NIEKKLNQNS RSEAPNWATQ DSGFY 445
    Length:445
    Mass (Da):52,221
    Last modified:July 19, 2005 - v1
    Checksum:iA5368651DD0DDD0C
    GO

    Sequence cautioni

    The sequence BAE00293.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB168168 mRNA. Translation: BAE00293.1. Different initiation.
    AB169215 mRNA. Translation: BAE01307.1.
    UniGeneiMfa.3647.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB168168 mRNA. Translation: BAE00293.1 . Different initiation.
    AB169215 mRNA. Translation: BAE01307.1 .
    UniGenei Mfa.3647.

    3D structure databases

    ProteinModelPortali Q4R6G8.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q4R6G8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG001097.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    HAMAPi MF_03004. eIF3e.
    InterProi IPR016650. eIF3e.
    IPR019010. eIF3e_N.
    IPR000717. PCI_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    PANTHERi PTHR10317. PTHR10317. 1 hit.
    Pfami PF09440. eIF3_N. 1 hit.
    PF01399. PCI. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016255. eIF3e_su6. 1 hit.
    SMARTi SM00088. PINT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
      International consortium for macaque cDNA sequencing and analysis
      Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiEIF3E_MACFA
    AccessioniPrimary (citable) accession number: Q4R6G8
    Secondary accession number(s): Q4R9C8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 26, 2007
    Last sequence update: July 19, 2005
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. Translation initiation factors
      List of translation initiation factor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3