Q4R6G8 (EIF3E_MACFA) Reviewed, UniProtKB/Swiss-Prot
Last modified November 13, 2013. Version 48. History...
Names and origin
|Protein names||Recommended name:|
Eukaryotic translation initiation factor 3 subunit E
Eukaryotic translation initiation factor 3 subunit 6
|Organism||Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)|
|Taxonomic identifier||9541 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca|
|Sequence length||445 AA.|
|Sequence processing||The displayed sequence is further processed into a mature form.|
|Protein existence||Evidence at transcript level|
General annotation (Comments)
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins By similarity. HAMAP-Rule MF_03004
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with COPS3, COPS6, COPS7 (COPS7A or COPS7B), EIF4G1, EPAS1, MCM7, NCBP1, PSMC6, TRIM27 and UPF2 By similarity. Interacts with IFIT1 and IFIT2 By similarity.
Belongs to the eIF-3 subunit E family.
Contains 1 PCI domain.
The sequence BAE00293.1 differs from that shown. Reason: Erroneous initiation.
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Initiator methionine||1||1||Removed By similarity|
|Chain||2 – 445||444||Eukaryotic translation initiation factor 3 subunit E HAMAP-Rule MF_03004||PRO_0000291872|
|Domain||290 – 395||106||PCI|
|Region||4 – 128||125||Sufficient for interaction with EPAS1 By similarity|
|Region||9 – 195||187||Sufficient for interaction with TRIM27 By similarity|
|Region||351 – 445||95||Sufficient for interaction with MCM7 By similarity|
Amino acid modifications
|Modified residue||2||1||N-acetylalanine By similarity|
|Modified residue||399||1||Phosphoserine By similarity|
|Modified residue||439||1||Phosphothreonine By similarity|
|Modified residue||445||1||Phosphotyrosine By similarity|
|||"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."|
International consortium for macaque cDNA sequencing and analysis
Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
|AB168168 mRNA. Translation: BAE00293.1. Different initiation.|
AB169215 mRNA. Translation: BAE01307.1.
3D structure databases
Protocols and materials databases
Family and domain databases
|Gene3D||22.214.171.124. 1 hit. |
|HAMAP||MF_03004. eIF3e. |
|InterPro||IPR016650. eIF3e. |
|PANTHER||PTHR10317. PTHR10317. 1 hit. |
|Pfam||PF09440. eIF3_N. 1 hit. |
PF01399. PCI. 1 hit.
|PIRSF||PIRSF016255. eIF3e_su6. 1 hit. |
|SMART||SM00088. PINT. 1 hit. |
|Accession||Primary (citable) accession number: Q4R6G8|
Secondary accession number(s): Q4R9C8
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|