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Q4R6G8 (EIF3E_MACFA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Eukaryotic translation initiation factor 3 subunit E
Short name=eIF3e
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 6
eIF-3 p48
Gene names
Name:EIF3E
Synonyms:EIF3S6, INT6
ORF Names:QtsA-10282, QtsA-18056
OrganismMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifier9541 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins By similarity. HAMAP-Rule MF_03004

Subunit structure

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with COPS3, COPS6, COPS7 (COPS7A or COPS7B), EIF4G1, EPAS1, MCM7, NCBP1, PSMC6, TRIM27 and UPF2 By similarity. Interacts with IFIT1 and IFIT2 By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus › PML body By similarity.

Post-translational modification

Phosphorylated upon DNA damage, probably by ATM or ATR By similarity. HAMAP-Rule MF_03004

Sequence similarities

Belongs to the eIF-3 subunit E family.

Contains 1 PCI domain.

Sequence caution

The sequence BAE00293.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 445444Eukaryotic translation initiation factor 3 subunit E HAMAP-Rule MF_03004
PRO_0000291872

Regions

Domain290 – 395106PCI
Region4 – 128125Sufficient for interaction with EPAS1 By similarity
Region9 – 195187Sufficient for interaction with TRIM27 By similarity
Region351 – 44595Sufficient for interaction with MCM7 By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue2751N6-acetyllysine By similarity
Modified residue3991Phosphoserine By similarity
Modified residue4391Phosphothreonine By similarity
Modified residue4451Phosphotyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4R6G8 [UniParc].

Last modified July 19, 2005. Version 1.
Checksum: A5368651DD0DDD0C

FASTA44552,221
        10         20         30         40         50         60 
MAEYDLTTRI AHFLDRHLVF PLLEFLSVKE IYNEKELLQG KLDLLSDTNM VDFAMDVYKN 

        70         80         90        100        110        120 
LYSDDIPHAL REKRTTVVAQ LKQLQAETEP IVKMFEDPET TRQMQSTRDG RMLFDYLADK 

       130        140        150        160        170        180 
HGFRQEYLDT LYRYAKFQYE CGNYSGAAEY LYFFRVLVPA TDRNALSSLW GKLASEILMQ 

       190        200        210        220        230        240 
NWDAAMEDLT RLKETIDNNS VSSPLQSLQQ RTWLIHWSLF VFFNHPKGRD NIIDLFLYQP 

       250        260        270        280        290        300 
QYLNAIQTMC PHILRYLTTA VITNKDVRKR RQVLKDLVKV IQQESYTYKD PITEFVECLY 

       310        320        330        340        350        360 
VNFDFDGAQK KLRECESVLV NDFFLVACLE DFIENARLFI FETFCRIHQC ISINMLADKL 

       370        380        390        400        410        420 
NMTPEEAERW IVNLIRNARL DAKIDSKLGH VVMGNNAVSP YQQVIEKTKS LSFRSQMLAM 

       430        440 
NIEKKLNQNS RSEAPNWATQ DSGFY

« Hide

References

[1]"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
International consortium for macaque cDNA sequencing and analysis
Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB168168 mRNA. Translation: BAE00293.1. Different initiation.
AB169215 mRNA. Translation: BAE01307.1.

3D structure databases

ProteinModelPortalQ4R6G8.
SMRQ4R6G8. Positions 326-399.
ModBaseSearch...

Proteomic databases

PRIDEQ4R6G8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG001097.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
HAMAPMF_03004. eIF3e.
InterProIPR019010. eIF3_su6_N.
IPR000717. PCI_dom.
IPR016650. Transl_init_fac_3_su6_euk.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF09440. eIF3_N. 1 hit.
PF01399. PCI. 1 hit.
[Graphical view]
PIRSFPIRSF016255. eIF3e_su6. 1 hit.
SMARTSM00088. PINT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameEIF3E_MACFA
AccessionPrimary (citable) accession number: Q4R6G8
Secondary accession number(s): Q4R9C8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: July 19, 2005
Last modified: May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Translation initiation factors

List of translation initiation factor entries

SIMILARITY comments

Index of protein domains and families

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