Q4R6D3 (UBP50_MACFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative ubiquitin carboxyl-terminal hydrolase 50
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 50
Ubiquitin thioesterase 50
Ubiquitin-specific-processing protease 50

Gene names

Name:	USP50
ORF Names:	QtsA-18237

Organism

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Cercopithecoidea › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Miscellaneous	The human ortholog is inactive.
Sequence similarities	Belongs to the peptidase C19 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 373	373	Putative ubiquitin carboxyl-terminal hydrolase 50		PRO_0000249528
Sites
	Active site	53	1	Nucleophile By similarity
	Active site	322	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4R6D3 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 09C80C792C8E4FE4	FASTA	373	42,752
	10 20 30 40 50 60 MTSQRSLPAD DFGIYYVLAE CTDYYDTLPV KEADGSQPCS QGVTGLRNLG NTCYMNAILQ 70 80 90 100 110 120 CLCSISPLVE YFLSGKYITA LQNDCSEVAT AFAYLMTDMW LGDSDCVSPE IFRSALGNLY 130 140 150 160 170 180 PAFTKKTQQD AQEFLIYVLN ELHEALKKYH YPRRRSHEKG SAQRCCRKWI TTETSVITQL 190 200 210 220 230 240 FEGQLNYSIV CLKCEKCTYK NEVFTVLSLP IPSEYECSLQ DCLQCFFQQD TLTWNNQIHC 250 260 270 280 290 300 SFCETKQETA VRAGISKAPK IIIFHLKRFD IQGTTKRKLR TDIHYPLTNL DLTPYICPIF 310 320 330 340 350 360 RKYPKYNLCA VVNHFGDLDG GHYTAFCKNS FTQAWYSFDD TRVSEIPDTS VQNATAYLLF 370 YSCQPFSIPI QKH

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB169252 mRNA. Translation: BAE01342.1.
3D structure databases
HSSP	HSSP built from PDB template 2AYN based on UniProtKB P54578.
ProteinModelPortal	Q4R6D3.
ModBase	Search...
Protein family/group databases
MEROPS	C19.058.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG098592.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP50_MACFA

Accession

Primary (citable) accession number: Q4R6D3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	July 19, 2005
Last modified:	February 22, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program